Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation
Chicken is an important economic animal that encounter various stressors including high temperature, high stocking density, bacterial infections and transportation, etc. affecting the poultry production with serious economic loss. To be in response to the varied stimulus, the hypothalamic-pituitary-...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010782 |
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| author | Yiping Wan Jiannan Zhang Xiao Zhang Jiliang He Ningkun Shi Yuanyou Li Juan Li Yajun Wang |
| author_facet | Yiping Wan Jiannan Zhang Xiao Zhang Jiliang He Ningkun Shi Yuanyou Li Juan Li Yajun Wang |
| author_sort | Yiping Wan |
| collection | DOAJ |
| description | Chicken is an important economic animal that encounter various stressors including high temperature, high stocking density, bacterial infections and transportation, etc. affecting the poultry production with serious economic loss. To be in response to the varied stimulus, the hypothalamic-pituitary-adrenal (HPA) axis is activated through the controlling of the synthesis and secretion of glucocorticoids (GCs). Present study characterized a novel gene C5H11ORF96, that demonstrated significant upregulation after the DEX injection in chicken, which simulates the stress stimulus. Our results showed that: (1) cC5H11ORF96 cDNA encodes a 120 amino acids protein, which shares high sequence identity with that of birds, mammals, reptiles, frogs and fish; (2) cC5H11ORF96 has a fully conserved RFKTQP motif and high proportion of serine, indicating its multiple potential phosphorylation sites; (3) cC5H11ORF96 is widely expressed in various chicken tissues, with high expression levels in the parathyroid gland, adrenal gland, and pituitary; (4) glucocorticoids (GCs) and stress significantly upregulate C5H11ORF96 mRNA and protein expression in the chicken pituitary and hypothalamus, suggesting its involvement in regulating stress response by influencing the negative feedback of GCs on the HPA axis in chickens. The characterization of the C5H11ORF96 gene in the chicken stress response provides potential targets for stress adaptability and poultry production. Meanwhile, our finding provides essential insights into the physiological functions of C11ORF96 gene in vertebrates. |
| format | Article |
| id | doaj-art-d1191f83fe8d40358100054746f5026f |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-d1191f83fe8d40358100054746f5026f2025-01-22T05:40:16ZengElsevierPoultry Science0032-57912025-01-011041104500Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulationYiping Wan0Jiannan Zhang1Xiao Zhang2Jiliang He3Ningkun Shi4Yuanyou Li5Juan Li6Yajun Wang7Key laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR ChinaKey laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, PR China; Correspondence author at: Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China.Chicken is an important economic animal that encounter various stressors including high temperature, high stocking density, bacterial infections and transportation, etc. affecting the poultry production with serious economic loss. To be in response to the varied stimulus, the hypothalamic-pituitary-adrenal (HPA) axis is activated through the controlling of the synthesis and secretion of glucocorticoids (GCs). Present study characterized a novel gene C5H11ORF96, that demonstrated significant upregulation after the DEX injection in chicken, which simulates the stress stimulus. Our results showed that: (1) cC5H11ORF96 cDNA encodes a 120 amino acids protein, which shares high sequence identity with that of birds, mammals, reptiles, frogs and fish; (2) cC5H11ORF96 has a fully conserved RFKTQP motif and high proportion of serine, indicating its multiple potential phosphorylation sites; (3) cC5H11ORF96 is widely expressed in various chicken tissues, with high expression levels in the parathyroid gland, adrenal gland, and pituitary; (4) glucocorticoids (GCs) and stress significantly upregulate C5H11ORF96 mRNA and protein expression in the chicken pituitary and hypothalamus, suggesting its involvement in regulating stress response by influencing the negative feedback of GCs on the HPA axis in chickens. The characterization of the C5H11ORF96 gene in the chicken stress response provides potential targets for stress adaptability and poultry production. Meanwhile, our finding provides essential insights into the physiological functions of C11ORF96 gene in vertebrates.http://www.sciencedirect.com/science/article/pii/S0032579124010782chickenC5H11ORF96glucocorticoidsstress |
| spellingShingle | Yiping Wan Jiannan Zhang Xiao Zhang Jiliang He Ningkun Shi Yuanyou Li Juan Li Yajun Wang Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation Poultry Science chicken C5H11ORF96 glucocorticoids stress |
| title | Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation |
| title_full | Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation |
| title_fullStr | Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation |
| title_full_unstemmed | Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation |
| title_short | Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation |
| title_sort | characterization of the c5h11orf96 gene in chickens cloning tissue distribution and investigation of its potential function in stress response regulation |
| topic | chicken C5H11ORF96 glucocorticoids stress |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010782 |
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