Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia
Fibromyalgia (FM), characterized by chronic widespread pain, fatigue, and cognitive/mood disturbances, leads to reduced workplace productivity and increased healthcare expenses. To determine if acquired epigenetic/genetic changes are associated with FM, we compared the frequency of spontaneously occ...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Nursing Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/795784 |
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| author | Victoria Menzies Debra E. Lyon Kellie J. Archer Qing Zhou Jenni Brumelle Kimberly H. Jones G. Gao Timothy P. York Colleen Jackson-Cook |
| author_facet | Victoria Menzies Debra E. Lyon Kellie J. Archer Qing Zhou Jenni Brumelle Kimberly H. Jones G. Gao Timothy P. York Colleen Jackson-Cook |
| author_sort | Victoria Menzies |
| collection | DOAJ |
| description | Fibromyalgia (FM), characterized by chronic widespread pain, fatigue, and cognitive/mood disturbances, leads to reduced workplace productivity and increased healthcare expenses. To determine if acquired epigenetic/genetic changes are associated with FM, we compared the frequency of spontaneously occurring micronuclei (MN) and genome-wide methylation patterns in women with FM (n=10) to those seen in comparably aged healthy controls (n=42 (MN); n=8 (methylation)). The mean (sd) MN frequency of women with FM (51.4 (21.9)) was significantly higher than that of controls (15.8 (8.5)) (χ2=45.552; df = 1; P=1.49×10-11). Significant differences (n=69 sites) in methylation patterns were observed between cases and controls considering a 5% false discovery rate. The majority of differentially methylated (DM) sites (91%) were attributable to increased values in the women with FM. The DM sites included significant biological clusters involved in neuron differentiation/nervous system development, skeletal/organ system development, and chromatin compaction. Genes associated with DM sites whose function has particular relevance to FM included BDNF, NAT15, HDAC4, PRKCA, RTN1, and PRKG1. Results support the need for future research to further examine the potential role of epigenetic and acquired chromosomal alterations as a possible biological mechanism underlying FM. |
| format | Article |
| id | doaj-art-d10a68fb724c4745ab6e6f4f1c8a31bb |
| institution | Kabale University |
| issn | 2090-1429 2090-1437 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Nursing Research and Practice |
| spelling | doaj-art-d10a68fb724c4745ab6e6f4f1c8a31bb2025-02-03T06:07:27ZengWileyNursing Research and Practice2090-14292090-14372013-01-01201310.1155/2013/795784795784Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with FibromyalgiaVictoria Menzies0Debra E. Lyon1Kellie J. Archer2Qing Zhou3Jenni Brumelle4Kimberly H. Jones5G. Gao6Timothy P. York7Colleen Jackson-Cook8Virginia Commonwealth University School of Nursing, 1100 East Leigh Street, Richmond, VA 23298-0567, USAVirginia Commonwealth University School of Nursing, 1100 East Leigh Street, Richmond, VA 23298-0567, USADepartment of Biostatistics, Virginia Commonwealth University, 830 East Main Street, Richmond, VA 23298, USADepartment of Biostatistics, Virginia Commonwealth University, 830 East Main Street, Richmond, VA 23298, USADepartment of Pathology, Virginia Commonwealth University, P.O. Box 980662, Richmond, VA 23298-0662, USADepartment of Pathology, Virginia Commonwealth University, P.O. Box 980662, Richmond, VA 23298-0662, USADepartment of Biostatistics, Virginia Commonwealth University, 830 East Main Street, Richmond, VA 23298, USADepartment of Human and Molecular Genetics, Virginia Commonwealth University, P.O. Box 980033, Richmond, VA 23298-0003, USAMassey Cancer Center, Virginia Commonwealth University, VA 23298-0037, USAFibromyalgia (FM), characterized by chronic widespread pain, fatigue, and cognitive/mood disturbances, leads to reduced workplace productivity and increased healthcare expenses. To determine if acquired epigenetic/genetic changes are associated with FM, we compared the frequency of spontaneously occurring micronuclei (MN) and genome-wide methylation patterns in women with FM (n=10) to those seen in comparably aged healthy controls (n=42 (MN); n=8 (methylation)). The mean (sd) MN frequency of women with FM (51.4 (21.9)) was significantly higher than that of controls (15.8 (8.5)) (χ2=45.552; df = 1; P=1.49×10-11). Significant differences (n=69 sites) in methylation patterns were observed between cases and controls considering a 5% false discovery rate. The majority of differentially methylated (DM) sites (91%) were attributable to increased values in the women with FM. The DM sites included significant biological clusters involved in neuron differentiation/nervous system development, skeletal/organ system development, and chromatin compaction. Genes associated with DM sites whose function has particular relevance to FM included BDNF, NAT15, HDAC4, PRKCA, RTN1, and PRKG1. Results support the need for future research to further examine the potential role of epigenetic and acquired chromosomal alterations as a possible biological mechanism underlying FM.http://dx.doi.org/10.1155/2013/795784 |
| spellingShingle | Victoria Menzies Debra E. Lyon Kellie J. Archer Qing Zhou Jenni Brumelle Kimberly H. Jones G. Gao Timothy P. York Colleen Jackson-Cook Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia Nursing Research and Practice |
| title | Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia |
| title_full | Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia |
| title_fullStr | Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia |
| title_full_unstemmed | Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia |
| title_short | Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia |
| title_sort | epigenetic alterations and an increased frequency of micronuclei in women with fibromyalgia |
| url | http://dx.doi.org/10.1155/2013/795784 |
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