Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus
It is known that sex hormones regulate IgA and IgG levels in the female reproductive tract. Moreover, antigen presentation by uterine and vaginal epithelial cells is also under strict hormonal control. The effect of the estrous cycle on cytokine secretion by va...
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| Format: | Article |
| Language: | English |
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Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520500141010 |
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| author | Maria C. Lopez Margaret A. Stanley |
| author_facet | Maria C. Lopez Margaret A. Stanley |
| author_sort | Maria C. Lopez |
| collection | DOAJ |
| description | It is known that sex hormones regulate IgA and IgG levels in the female
reproductive tract. Moreover, antigen presentation by uterine and vaginal epithelial
cells is also under strict hormonal control. The effect of the estrous cycle on
cytokine secretion by vaginal and uterine lymphoid cells has been examined in
mice using simultaneous staining for cytoplasmic cytokines and surface markers
after ex vivo culture with PMA/ionomycin in the presence of Brefeldin A, and flow
cytometry analysis. Two different mice strains, BALB/c and C57BL/6 mice, were
used. The most relevant finding was the increase in the proportion of vaginal cells
secreting IFN-γ at diestrus in both strains of mice. Other cytokines (IL-2 and IL-4) as
well as some T cell subsets seemed to be modified in a strain dependent
fashion. Data also suggest that NK cells are at least partially responsible for
IFN-γ secretion. Our data indicate that vaginal and uterus lymphoid cells isolated
at diestrus were in vivo activated to secrete cytokines
after ex vivo
culture. IFN-γ seems to be the key cytokine, since it increases in both strains of mice. |
| format | Article |
| id | doaj-art-d0fca819fafe4ab096a46e4c7820e013 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-d0fca819fafe4ab096a46e4c7820e0132025-02-03T01:28:38ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-0112215916410.1080/17402520500141010Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and DiestrusMaria C. Lopez0Margaret A. Stanley1Department of Pathology, University of Cambridge, CB2 1QP, Cambridge, UKDepartment of Pathology, University of Cambridge, CB2 1QP, Cambridge, UKIt is known that sex hormones regulate IgA and IgG levels in the female reproductive tract. Moreover, antigen presentation by uterine and vaginal epithelial cells is also under strict hormonal control. The effect of the estrous cycle on cytokine secretion by vaginal and uterine lymphoid cells has been examined in mice using simultaneous staining for cytoplasmic cytokines and surface markers after ex vivo culture with PMA/ionomycin in the presence of Brefeldin A, and flow cytometry analysis. Two different mice strains, BALB/c and C57BL/6 mice, were used. The most relevant finding was the increase in the proportion of vaginal cells secreting IFN-γ at diestrus in both strains of mice. Other cytokines (IL-2 and IL-4) as well as some T cell subsets seemed to be modified in a strain dependent fashion. Data also suggest that NK cells are at least partially responsible for IFN-γ secretion. Our data indicate that vaginal and uterus lymphoid cells isolated at diestrus were in vivo activated to secrete cytokines after ex vivo culture. IFN-γ seems to be the key cytokine, since it increases in both strains of mice.http://dx.doi.org/10.1080/17402520500141010 |
| spellingShingle | Maria C. Lopez Margaret A. Stanley Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus Clinical and Developmental Immunology |
| title | Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus |
| title_full | Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus |
| title_fullStr | Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus |
| title_full_unstemmed | Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus |
| title_short | Cytokine Profile of Mouse Vaginal and Uterus Lymphocytes at Estrus and Diestrus |
| title_sort | cytokine profile of mouse vaginal and uterus lymphocytes at estrus and diestrus |
| url | http://dx.doi.org/10.1080/17402520500141010 |
| work_keys_str_mv | AT mariaclopez cytokineprofileofmousevaginalanduteruslymphocytesatestrusanddiestrus AT margaretastanley cytokineprofileofmousevaginalanduteruslymphocytesatestrusanddiestrus |