Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor

Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not bee...

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Main Authors: Eliška Špaleková, Alexander V. Makarevich, Norbert Lukáč
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Veterinary Medicine International
Online Access:http://dx.doi.org/10.4061/2011/642931
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author Eliška Špaleková
Alexander V. Makarevich
Norbert Lukáč
author_facet Eliška Špaleková
Alexander V. Makarevich
Norbert Lukáč
author_sort Eliška Špaleková
collection DOAJ
description Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EGF addition. EGF was added to chilled ram sperm at concentrations of 0, 100, 200, and 400 ng⋅ml−1. Analyses were realized using computer, assisted semen analyzer (CASA)—Hamilton Thorn motility analyzer (version 7). The effect of EGF was already visible after 30 min of incubation. Significant effect on ram sperm total motility and progressive movement was observed at higher EGF concentrations after 48 h of incubation. Our results show that EGF affects sperm motility parameters depending on concentration and time of exposure.
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spelling doaj-art-d0f1dfb2b00d4700a4bcf7e07945f12d2025-08-20T02:19:54ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/642931642931Ram Sperm Motility Parameters under The Influence of Epidermal Growth FactorEliška Špaleková0Alexander V. Makarevich1Norbert Lukáč2Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture Nitra, Trieda A. Hlinku 2, 949 76 Nitra, SlovakiaInstitute for Farm Animal Genetics and Reproduction, Animal Production Research Centre Nitra, Hlohovecká 2, 95141 Nitra, SlovakiaDepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture Nitra, Trieda A. Hlinku 2, 949 76 Nitra, SlovakiaEpidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EGF addition. EGF was added to chilled ram sperm at concentrations of 0, 100, 200, and 400 ng⋅ml−1. Analyses were realized using computer, assisted semen analyzer (CASA)—Hamilton Thorn motility analyzer (version 7). The effect of EGF was already visible after 30 min of incubation. Significant effect on ram sperm total motility and progressive movement was observed at higher EGF concentrations after 48 h of incubation. Our results show that EGF affects sperm motility parameters depending on concentration and time of exposure.http://dx.doi.org/10.4061/2011/642931
spellingShingle Eliška Špaleková
Alexander V. Makarevich
Norbert Lukáč
Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
Veterinary Medicine International
title Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
title_full Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
title_fullStr Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
title_full_unstemmed Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
title_short Ram Sperm Motility Parameters under The Influence of Epidermal Growth Factor
title_sort ram sperm motility parameters under the influence of epidermal growth factor
url http://dx.doi.org/10.4061/2011/642931
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