Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure
Summary: The microenvironmental changes in peritoneal dialysis effluent (PDE) after long-term vintage (LV) of PD in patients with ultrafiltration failure (LV_UF) are unclear. Single-cell sequencing revealed that peritoneal neutrophils were elevated in LV_UF patients, while MRC1-macrophage subcluster...
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Elsevier
2024-12-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224026087 |
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| author | Xiangwen Diao Cuixia Zhan Hongjian Ye Haishan Wu Chunyan Yi Jianxiong Lin Haiping Mao Wei Chen Xiao Yang |
| author_facet | Xiangwen Diao Cuixia Zhan Hongjian Ye Haishan Wu Chunyan Yi Jianxiong Lin Haiping Mao Wei Chen Xiao Yang |
| author_sort | Xiangwen Diao |
| collection | DOAJ |
| description | Summary: The microenvironmental changes in peritoneal dialysis effluent (PDE) after long-term vintage (LV) of PD in patients with ultrafiltration failure (LV_UF) are unclear. Single-cell sequencing revealed that peritoneal neutrophils were elevated in LV_UF patients, while MRC1-macrophage subcluster decreased compared with PD patients with short vintage (SV) and LV without ultrafiltration failure (LV_NOT_UF). Compared with the LV_NOT_UF group, the upregulated differentially expressed genes (DEGs) of monocytes/macrophages in the LV_UF group were involved in inflammatory response and EMT progress. LV_UF patients had a higher proportion of epithelial-like mesothelial cells (E-MCs), which were characterized by autophagy activation, inflammation, and upregulation of neutrophil- and autophagy-related DEGs compared to the LV_NOT_UF group. Additionally, mesenchymal-like MCs and AQP1 expression were reduced in the LV_UF group compared with the other groups. Both neutrophils and monocytes/macrophages interacted with MCs. Our study provides insights into the roles of peritoneal mesothelial cells and inflammatory cells in PD patients with UF. |
| format | Article |
| id | doaj-art-d0cd8c04b843483bab8fd24f9cf8cf75 |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-d0cd8c04b843483bab8fd24f9cf8cf752025-08-20T02:35:03ZengElsevieriScience2589-00422024-12-01271211138310.1016/j.isci.2024.111383Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failureXiangwen Diao0Cuixia Zhan1Hongjian Ye2Haishan Wu3Chunyan Yi4Jianxiong Lin5Haiping Mao6Wei Chen7Xiao Yang8Department of Emergency, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, ChinaDepartment of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China; NHC Key Laboratory of Clinical Nephrology (Sun Yat-Sen University) and Guangdong Provincial Key Laboratory of Nephrology, Guangzhou 510080, Guangdong, China; Corresponding authorSummary: The microenvironmental changes in peritoneal dialysis effluent (PDE) after long-term vintage (LV) of PD in patients with ultrafiltration failure (LV_UF) are unclear. Single-cell sequencing revealed that peritoneal neutrophils were elevated in LV_UF patients, while MRC1-macrophage subcluster decreased compared with PD patients with short vintage (SV) and LV without ultrafiltration failure (LV_NOT_UF). Compared with the LV_NOT_UF group, the upregulated differentially expressed genes (DEGs) of monocytes/macrophages in the LV_UF group were involved in inflammatory response and EMT progress. LV_UF patients had a higher proportion of epithelial-like mesothelial cells (E-MCs), which were characterized by autophagy activation, inflammation, and upregulation of neutrophil- and autophagy-related DEGs compared to the LV_NOT_UF group. Additionally, mesenchymal-like MCs and AQP1 expression were reduced in the LV_UF group compared with the other groups. Both neutrophils and monocytes/macrophages interacted with MCs. Our study provides insights into the roles of peritoneal mesothelial cells and inflammatory cells in PD patients with UF.http://www.sciencedirect.com/science/article/pii/S2589004224026087Body substance sampleIntegrative aspects of cell biologyMicroenvironmentTranscriptomics |
| spellingShingle | Xiangwen Diao Cuixia Zhan Hongjian Ye Haishan Wu Chunyan Yi Jianxiong Lin Haiping Mao Wei Chen Xiao Yang Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure iScience Body substance sample Integrative aspects of cell biology Microenvironment Transcriptomics |
| title | Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure |
| title_full | Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure |
| title_fullStr | Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure |
| title_full_unstemmed | Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure |
| title_short | Single-cell transcriptomic reveals the peritoneal microenvironmental change in long-term peritoneal dialysis patients with ultrafiltration failure |
| title_sort | single cell transcriptomic reveals the peritoneal microenvironmental change in long term peritoneal dialysis patients with ultrafiltration failure |
| topic | Body substance sample Integrative aspects of cell biology Microenvironment Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224026087 |
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