RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate
Objective: Neodymium nitrate (Nd(NO<sub>3</sub>)<sub>3</sub>) is widely used globally, raising concerns about its occupational and environmental safety. It enters the human body via the digestive system, accumulates in organs, and causes toxicity, including potential hepatoto...
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MDPI AG
2025-07-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/13/7/573 |
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| author | Ning Wang Jing Leng Yaxin Han Gonghua Tao Jingqiu Sun Cheng Dong Kelei Qian Xiuli Chang Ping Xiao Xinyu Hong |
| author_facet | Ning Wang Jing Leng Yaxin Han Gonghua Tao Jingqiu Sun Cheng Dong Kelei Qian Xiuli Chang Ping Xiao Xinyu Hong |
| author_sort | Ning Wang |
| collection | DOAJ |
| description | Objective: Neodymium nitrate (Nd(NO<sub>3</sub>)<sub>3</sub>) is widely used globally, raising concerns about its occupational and environmental safety. It enters the human body via the digestive system, accumulates in organs, and causes toxicity, including potential hepatotoxicity. However, the role of non-coding RNAs (ncRNAs) in Nd(NO<sub>3</sub>)<sub>3</sub>-induced liver injury remains unclear. This study aimed to identify key genes and regulatory pathways involved in Nd(NO<sub>3</sub>)<sub>3</sub>-induced hepatic injury using RNA sequencing (RNA-seq) and differential gene expression analysis. Methods: Mouse hepatocytes (AML12 cells) were exposed to Nd(NO<sub>3</sub>)<sub>3</sub>, and RNA-seq was performed to analyze the expression profiles of miRNA, lncRNA, circRNA, and mRNA. qPCR was used to validate the RNA-seq results. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted to explore the functions and pathways associated with differentially expressed genes (DEGs). Results: Nd(NO<sub>3</sub>)<sub>3</sub> exposure altered the expression of ferroptosis-related genes and induced significant changes in mRNA, miRNA, circRNA, and lncRNA expression levels. GO and KEGG analyses revealed that DEGs were closely related to cellular ferroptosis pathways. Specific miRNAs, lncRNAs, and circRNAs were significantly upregulated, suggesting their potential as biomarkers for Nd(NO<sub>3</sub>)<sub>3</sub>-induced ferroptosis and liver injury. Conclusion: This study provides the first comprehensive transcriptome database for Nd(NO<sub>3</sub>)<sub>3</sub>-induced liver injury, highlighting the involvement of ncRNAs in hepatotoxicity. These findings offer valuable insights for developing biomarkers and understanding the mechanisms underlying Nd(NO<sub>3</sub>)<sub>3</sub>-induced hepatic injury. |
| format | Article |
| id | doaj-art-d0b56ed9ea214ebda4e80f004e6d3bcf |
| institution | DOAJ |
| issn | 2305-6304 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Toxics |
| spelling | doaj-art-d0b56ed9ea214ebda4e80f004e6d3bcf2025-08-20T02:47:07ZengMDPI AGToxics2305-63042025-07-0113757310.3390/toxics13070573RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium NitrateNing Wang0Jing Leng1Yaxin Han2Gonghua Tao3Jingqiu Sun4Cheng Dong5Kelei Qian6Xiuli Chang7Ping Xiao8Xinyu Hong9Institute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaSchool of Public Health, Fudan University, Shanghai 200032, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaSchool of Public Health, Fudan University, Shanghai 200032, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaInstitute of Chemical Toxicity Testing, NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 201701, ChinaObjective: Neodymium nitrate (Nd(NO<sub>3</sub>)<sub>3</sub>) is widely used globally, raising concerns about its occupational and environmental safety. It enters the human body via the digestive system, accumulates in organs, and causes toxicity, including potential hepatotoxicity. However, the role of non-coding RNAs (ncRNAs) in Nd(NO<sub>3</sub>)<sub>3</sub>-induced liver injury remains unclear. This study aimed to identify key genes and regulatory pathways involved in Nd(NO<sub>3</sub>)<sub>3</sub>-induced hepatic injury using RNA sequencing (RNA-seq) and differential gene expression analysis. Methods: Mouse hepatocytes (AML12 cells) were exposed to Nd(NO<sub>3</sub>)<sub>3</sub>, and RNA-seq was performed to analyze the expression profiles of miRNA, lncRNA, circRNA, and mRNA. qPCR was used to validate the RNA-seq results. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted to explore the functions and pathways associated with differentially expressed genes (DEGs). Results: Nd(NO<sub>3</sub>)<sub>3</sub> exposure altered the expression of ferroptosis-related genes and induced significant changes in mRNA, miRNA, circRNA, and lncRNA expression levels. GO and KEGG analyses revealed that DEGs were closely related to cellular ferroptosis pathways. Specific miRNAs, lncRNAs, and circRNAs were significantly upregulated, suggesting their potential as biomarkers for Nd(NO<sub>3</sub>)<sub>3</sub>-induced ferroptosis and liver injury. Conclusion: This study provides the first comprehensive transcriptome database for Nd(NO<sub>3</sub>)<sub>3</sub>-induced liver injury, highlighting the involvement of ncRNAs in hepatotoxicity. These findings offer valuable insights for developing biomarkers and understanding the mechanisms underlying Nd(NO<sub>3</sub>)<sub>3</sub>-induced hepatic injury.https://www.mdpi.com/2305-6304/13/7/573neodymium nitrateRNA sequencingferroptosishepatic injurybiomarkers |
| spellingShingle | Ning Wang Jing Leng Yaxin Han Gonghua Tao Jingqiu Sun Cheng Dong Kelei Qian Xiuli Chang Ping Xiao Xinyu Hong RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate Toxics neodymium nitrate RNA sequencing ferroptosis hepatic injury biomarkers |
| title | RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate |
| title_full | RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate |
| title_fullStr | RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate |
| title_full_unstemmed | RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate |
| title_short | RNA-Seq Analysis of Mouse Hepatocytes AML12 Exposed to Neodymium Nitrate |
| title_sort | rna seq analysis of mouse hepatocytes aml12 exposed to neodymium nitrate |
| topic | neodymium nitrate RNA sequencing ferroptosis hepatic injury biomarkers |
| url | https://www.mdpi.com/2305-6304/13/7/573 |
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