Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences
This paper deals with development of signaling pathways models and using plasmid-based experiments to support parameter estimation. We show that if cells transfected with plasmids are used in experiments, the models should include additional components that describe explicitly effects induced by pla...
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| Format: | Article |
| Language: | English |
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AIMS Press
2016-07-01
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| Series: | Mathematical Biosciences and Engineering |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/mbe.2016039 |
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| _version_ | 1832590081104281600 |
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| author | Jaroslaw Smieja Marzena Dolbniak |
| author_facet | Jaroslaw Smieja Marzena Dolbniak |
| author_sort | Jaroslaw Smieja |
| collection | DOAJ |
| description | This paper deals with development of signaling pathways models and using plasmid-based experiments to support parameter estimation. We show that if cells transfected with plasmids are used in experiments, the models should include additional components that describe explicitly effects induced by plasmids. Otherwise, when the model is used to analyze responses of wild type, i.e. non-transfected cells, it may not capture their dynamics properly or even lead to false conclusions. In order to illustrate this, an original mathematical model of miRNA-mediated control of gene expression in the NF$\kappa$B pathway is presented. The paper shows what artifacts might appear due to experimental procedures and how to develop the models in order to avoid pursuing these artifacts instead of real kinetics. |
| format | Article |
| id | doaj-art-d0aa61896f4c4104bd1cf2d097465ffd |
| institution | Kabale University |
| issn | 1551-0018 |
| language | English |
| publishDate | 2016-07-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | Mathematical Biosciences and Engineering |
| spelling | doaj-art-d0aa61896f4c4104bd1cf2d097465ffd2025-01-24T02:37:49ZengAIMS PressMathematical Biosciences and Engineering1551-00182016-07-011361207122210.3934/mbe.2016039Sensitivity of signaling pathway dynamics to plasmid transfection and its consequencesJaroslaw Smieja0Marzena Dolbniak1Institute of Automatic Control, Silesian University of Technology, Akademicka 16, 44-101 GliwiceInstitute of Automatic Control, Silesian University of Technology, Akademicka 16, 44-100 GliwiceThis paper deals with development of signaling pathways models and using plasmid-based experiments to support parameter estimation. We show that if cells transfected with plasmids are used in experiments, the models should include additional components that describe explicitly effects induced by plasmids. Otherwise, when the model is used to analyze responses of wild type, i.e. non-transfected cells, it may not capture their dynamics properly or even lead to false conclusions. In order to illustrate this, an original mathematical model of miRNA-mediated control of gene expression in the NF$\kappa$B pathway is presented. The paper shows what artifacts might appear due to experimental procedures and how to develop the models in order to avoid pursuing these artifacts instead of real kinetics.https://www.aimspress.com/article/doi/10.3934/mbe.2016039sensitivity.plasmid transfectionsignaling pathways modeling |
| spellingShingle | Jaroslaw Smieja Marzena Dolbniak Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences Mathematical Biosciences and Engineering sensitivity. plasmid transfection signaling pathways modeling |
| title | Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| title_full | Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| title_fullStr | Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| title_full_unstemmed | Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| title_short | Sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| title_sort | sensitivity of signaling pathway dynamics to plasmid transfection and its consequences |
| topic | sensitivity. plasmid transfection signaling pathways modeling |
| url | https://www.aimspress.com/article/doi/10.3934/mbe.2016039 |
| work_keys_str_mv | AT jaroslawsmieja sensitivityofsignalingpathwaydynamicstoplasmidtransfectionanditsconsequences AT marzenadolbniak sensitivityofsignalingpathwaydynamicstoplasmidtransfectionanditsconsequences |