Optimization of Aflatoxin B1 Aptasensing
Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. De...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2017/2461354 |
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| author | Marzieh Jafari Mohsen Rezaei Heibatullah Kalantari Maryam Tabarzad Bahram Daraei |
| author_facet | Marzieh Jafari Mohsen Rezaei Heibatullah Kalantari Maryam Tabarzad Bahram Daraei |
| author_sort | Marzieh Jafari |
| collection | DOAJ |
| description | Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps. |
| format | Article |
| id | doaj-art-d092a664448d4e5fa8d7d6c3d2f6e42f |
| institution | DOAJ |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-d092a664448d4e5fa8d7d6c3d2f6e42f2025-08-20T03:19:37ZengWileyJournal of Toxicology1687-81911687-82052017-01-01201710.1155/2017/24613542461354Optimization of Aflatoxin B1 AptasensingMarzieh Jafari0Mohsen Rezaei1Heibatullah Kalantari2Maryam Tabarzad3Bahram Daraei4Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranProtein Technology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranCombination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps.http://dx.doi.org/10.1155/2017/2461354 |
| spellingShingle | Marzieh Jafari Mohsen Rezaei Heibatullah Kalantari Maryam Tabarzad Bahram Daraei Optimization of Aflatoxin B1 Aptasensing Journal of Toxicology |
| title | Optimization of Aflatoxin B1 Aptasensing |
| title_full | Optimization of Aflatoxin B1 Aptasensing |
| title_fullStr | Optimization of Aflatoxin B1 Aptasensing |
| title_full_unstemmed | Optimization of Aflatoxin B1 Aptasensing |
| title_short | Optimization of Aflatoxin B1 Aptasensing |
| title_sort | optimization of aflatoxin b1 aptasensing |
| url | http://dx.doi.org/10.1155/2017/2461354 |
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