Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos
Chlorpyrifos (CPF) is an organophosphate insecticide that is extensively utilized globally due to its effectiveness against over 200 pest species. CPF exhibits its toxicity primarily through the inhibition of the acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have als...
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2025-01-01
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| author | Yuxin Chen Ziwen Yang Zhiqiang Guo Lemei Zhan Kenneth B. Storey Danna Yu Jiayong Zhang |
| author_facet | Yuxin Chen Ziwen Yang Zhiqiang Guo Lemei Zhan Kenneth B. Storey Danna Yu Jiayong Zhang |
| author_sort | Yuxin Chen |
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| description | Chlorpyrifos (CPF) is an organophosphate insecticide that is extensively utilized globally due to its effectiveness against over 200 pest species. CPF exhibits its toxicity primarily through the inhibition of the acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have also been observed. The present study quantified the transcript levels of mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction (<i>RT-qPCR</i>) in samples of larvae of three dragonfly species (<i>A. parthenope</i>, <i>E. elegans</i>, and <i>G. confluens</i>) under different levels of CPF stress. By exposing larvae from uncontaminated populations to 0.05 μg/L CPF for 24 h, the transcript levels of seven mtPCGs in <i>A. parthenope</i> were significantly increased (<i>p</i> < 0.05) by 1.89 ± 0.42-fold for <i>COI</i>, 4.30 ± 0.24-fold for <i>COIII</i>, 5.94 ± 0.17-fold for <i>ND1</i>, 4.69 ± 0.56-fold for <i>ND2</i>, 3.44 ± 0.48-fold for <i>ND4</i>, 2.19 ± 0.53-fold for <i>ND4L</i>, and 5.05 ± 0.36-fold for <i>Cytb</i>, respectively. In <i>E. elegans</i>, the transcript levels of <i>ND1</i>, <i>ND2</i>, and <i>ND4</i> increased by 1.23 ± 0.15, 1.48 ± 0.31, and 1.98 ± 0.25-fold, respectively (<i>p</i> < 0.05). In <i>G. confluens</i>, the transcript levels of <i>COI</i>, <i>COIII</i>, and <i>ND4</i> increased by 1.56 ± 0.13, 1.50 ± 0.26, and 3.74 ± 0.40-fold, respectively (<i>p</i> < 0.01). It was demonstrated that the transcript levels of different mtPCGs showed significant up-regulation in the three different dragonfly larvae under CPF stress in the absence of mortality. <i>ND4</i> was significantly increased in all three species, indicating that it is an important target gene. The present study underscores the response of mitochondrial gene expression in larvae of three different species in response to CPF pollutants, indicating that pesticide influences can potentially alter mitochondrial gene expression and potentially act as a method for assessing aquatic ecosystem health. |
| format | Article |
| id | doaj-art-d0810894501e4a6bb6243e65ace1cc62 |
| institution | Kabale University |
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| language | English |
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| spelling | doaj-art-d0810894501e4a6bb6243e65ace1cc622025-01-24T13:35:50ZengMDPI AGInsects2075-44502025-01-011618510.3390/insects16010085Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of ChlorpyrifosYuxin Chen0Ziwen Yang1Zhiqiang Guo2Lemei Zhan3Kenneth B. Storey4Danna Yu5Jiayong Zhang6College of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaDepartment of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaChlorpyrifos (CPF) is an organophosphate insecticide that is extensively utilized globally due to its effectiveness against over 200 pest species. CPF exhibits its toxicity primarily through the inhibition of the acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have also been observed. The present study quantified the transcript levels of mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction (<i>RT-qPCR</i>) in samples of larvae of three dragonfly species (<i>A. parthenope</i>, <i>E. elegans</i>, and <i>G. confluens</i>) under different levels of CPF stress. By exposing larvae from uncontaminated populations to 0.05 μg/L CPF for 24 h, the transcript levels of seven mtPCGs in <i>A. parthenope</i> were significantly increased (<i>p</i> < 0.05) by 1.89 ± 0.42-fold for <i>COI</i>, 4.30 ± 0.24-fold for <i>COIII</i>, 5.94 ± 0.17-fold for <i>ND1</i>, 4.69 ± 0.56-fold for <i>ND2</i>, 3.44 ± 0.48-fold for <i>ND4</i>, 2.19 ± 0.53-fold for <i>ND4L</i>, and 5.05 ± 0.36-fold for <i>Cytb</i>, respectively. In <i>E. elegans</i>, the transcript levels of <i>ND1</i>, <i>ND2</i>, and <i>ND4</i> increased by 1.23 ± 0.15, 1.48 ± 0.31, and 1.98 ± 0.25-fold, respectively (<i>p</i> < 0.05). In <i>G. confluens</i>, the transcript levels of <i>COI</i>, <i>COIII</i>, and <i>ND4</i> increased by 1.56 ± 0.13, 1.50 ± 0.26, and 3.74 ± 0.40-fold, respectively (<i>p</i> < 0.01). It was demonstrated that the transcript levels of different mtPCGs showed significant up-regulation in the three different dragonfly larvae under CPF stress in the absence of mortality. <i>ND4</i> was significantly increased in all three species, indicating that it is an important target gene. The present study underscores the response of mitochondrial gene expression in larvae of three different species in response to CPF pollutants, indicating that pesticide influences can potentially alter mitochondrial gene expression and potentially act as a method for assessing aquatic ecosystem health.https://www.mdpi.com/2075-4450/16/1/85Chlorpyrifosmitochondrial gene transcript leveldragonfly |
| spellingShingle | Yuxin Chen Ziwen Yang Zhiqiang Guo Lemei Zhan Kenneth B. Storey Danna Yu Jiayong Zhang Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos Insects Chlorpyrifos mitochondrial gene transcript level dragonfly |
| title | Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos |
| title_full | Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos |
| title_fullStr | Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos |
| title_full_unstemmed | Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos |
| title_short | Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos |
| title_sort | mitochondrial gene expression of three different dragonflies under the stress of chlorpyrifos |
| topic | Chlorpyrifos mitochondrial gene transcript level dragonfly |
| url | https://www.mdpi.com/2075-4450/16/1/85 |
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