Hepatitis E virus infection among blood donors in Ningbo
[Objective] To investigate the infection status and characteristics of HEV among voluntary blood donors in Ningbo, and to provide a basis for improving the blood screening strategy. [Methods] A total of 12 227 blood samples from voluntary blood donors in Ningbo from June 2022 to May 2023 were tested...
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| Format: | Article |
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-01-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.002&lang=en |
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| _version_ | 1832585155026354176 |
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| author | PENG Mingxi LIU Yiyu MAO Huyan LIN Dan XIN Lu SHU Ning HAN Jianfeng DING Feng |
| author_facet | PENG Mingxi LIU Yiyu MAO Huyan LIN Dan XIN Lu SHU Ning HAN Jianfeng DING Feng |
| author_sort | PENG Mingxi |
| collection | DOAJ |
| description | [Objective] To investigate the infection status and characteristics of HEV among voluntary blood donors in Ningbo, and to provide a basis for improving the blood screening strategy. [Methods] A total of 12 227 blood samples from voluntary blood donors in Ningbo from June 2022 to May 2023 were tested for HEV serology, enzymology, and nucleic acid testing. Furthermore, HEV gene sequencing was performed for genotyping analysis, and donors with reactive nucleic acid testing results were followed up to confirm their infection status. [Results] The reactivity rate of HEV Ag, anti-HEV IgM and anti-HEV IgG was 0.098%, 0.899% and 29.198%, respectively. There was no difference in the reactivity of anti-HEV IgM and anti-HEV IgG between genders, donation frequencies and donation types (P>0.05). The reactivity rate increased significantly with age (P<0.05). The rate of ALT disqualification (ALT>50U/L) was significantly higher than that in non-reactive samples (P<0.05). The HEV Ag reactivity rate (0.098%) was not correlated with gender, donation frequency, donation type or age. One HEV RNA positive case was found, with a positive rate of 0.008%(1/12 227). It was confirmed to be hepatitis E virus genotype 3 by sequencing analysis. Apart from HEV Ag reactivity, all other blood safety screening items were non-reactive, suggesting this case might be in the acute infection phase. The follow-up results showed that all indicators of the donor's previous blood donation were non-reactive. [Conclusion] Pre-donation ALT detection can reduce the risk of transfusion-transmitted HEV (TT-HEV) to a certain extent, and the effective way to prevent TT-HEV is to detect HEV RNA and serology of donor blood. |
| format | Article |
| id | doaj-art-d0774df3667e4f8493977a14041df5b3 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-d0774df3667e4f8493977a14041df5b32025-01-27T01:46:11ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-01-0138171210.13303/j.cjbt.issn.1004-549x.2025.01.0021004-549X(2025)1-0007-06Hepatitis E virus infection among blood donors in NingboPENG Mingxi0LIU Yiyu1MAO Huyan2LIN Dan3XIN Lu4SHU Ning5HAN Jianfeng6DING Feng7Ningbo Central Blood Station, Ningbo 315040, ChinaNingbo Central Blood Station, Ningbo 315040, ChinaNingbo Central Blood Station, Ningbo 315040, ChinaNingbo Central Blood Station, Ningbo 315040, ChinaNingbo Central Blood Station, Ningbo 315040, ChinaNingbo Central Blood Station, Ningbo 315040, ChinaMedical Affairs Department, Sansure Biotech Inc, Changsha 410205, ChinaInstitute of Life Sciences, Sansure Biotech Inc, National and local Joint Engineering Research Center for Infectious Diseases and Tumor Gene Diagnosis Technology, Changsha 410205, China[Objective] To investigate the infection status and characteristics of HEV among voluntary blood donors in Ningbo, and to provide a basis for improving the blood screening strategy. [Methods] A total of 12 227 blood samples from voluntary blood donors in Ningbo from June 2022 to May 2023 were tested for HEV serology, enzymology, and nucleic acid testing. Furthermore, HEV gene sequencing was performed for genotyping analysis, and donors with reactive nucleic acid testing results were followed up to confirm their infection status. [Results] The reactivity rate of HEV Ag, anti-HEV IgM and anti-HEV IgG was 0.098%, 0.899% and 29.198%, respectively. There was no difference in the reactivity of anti-HEV IgM and anti-HEV IgG between genders, donation frequencies and donation types (P>0.05). The reactivity rate increased significantly with age (P<0.05). The rate of ALT disqualification (ALT>50U/L) was significantly higher than that in non-reactive samples (P<0.05). The HEV Ag reactivity rate (0.098%) was not correlated with gender, donation frequency, donation type or age. One HEV RNA positive case was found, with a positive rate of 0.008%(1/12 227). It was confirmed to be hepatitis E virus genotype 3 by sequencing analysis. Apart from HEV Ag reactivity, all other blood safety screening items were non-reactive, suggesting this case might be in the acute infection phase. The follow-up results showed that all indicators of the donor's previous blood donation were non-reactive. [Conclusion] Pre-donation ALT detection can reduce the risk of transfusion-transmitted HEV (TT-HEV) to a certain extent, and the effective way to prevent TT-HEV is to detect HEV RNA and serology of donor blood.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.002&lang=enblood donorshepatitis e virusblood screeningnucleic acid testinggenotyping |
| spellingShingle | PENG Mingxi LIU Yiyu MAO Huyan LIN Dan XIN Lu SHU Ning HAN Jianfeng DING Feng Hepatitis E virus infection among blood donors in Ningbo Zhongguo shuxue zazhi blood donors hepatitis e virus blood screening nucleic acid testing genotyping |
| title | Hepatitis E virus infection among blood donors in Ningbo |
| title_full | Hepatitis E virus infection among blood donors in Ningbo |
| title_fullStr | Hepatitis E virus infection among blood donors in Ningbo |
| title_full_unstemmed | Hepatitis E virus infection among blood donors in Ningbo |
| title_short | Hepatitis E virus infection among blood donors in Ningbo |
| title_sort | hepatitis e virus infection among blood donors in ningbo |
| topic | blood donors hepatitis e virus blood screening nucleic acid testing genotyping |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.002&lang=en |
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