Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes
In order to contribute to the fight against infectious diseases, the in vitro antibacterial activity and the antibiotic-potentiating effects of Tristemma hirtum and five other Cameroonian edible plants have been evaluated against Gram-negative multidrug-resistant (MDR) phenotypes. The microdilution...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/7651482 |
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| author | Gaëlle S. Nguenang Armelle T. Mbaveng Aimé G. Fankam Hermione T. Manekeng Paul Nayim Brice E. N. Wamba Victor Kuete |
| author_facet | Gaëlle S. Nguenang Armelle T. Mbaveng Aimé G. Fankam Hermione T. Manekeng Paul Nayim Brice E. N. Wamba Victor Kuete |
| author_sort | Gaëlle S. Nguenang |
| collection | DOAJ |
| description | In order to contribute to the fight against infectious diseases, the in vitro antibacterial activity and the antibiotic-potentiating effects of Tristemma hirtum and five other Cameroonian edible plants have been evaluated against Gram-negative multidrug-resistant (MDR) phenotypes. The microdilution method was used to evaluate the bacterial susceptibility of the extracts and their combination to common antibiotics. The phytochemical screening of the extracts was carried out according to standard methods. Phytochemical analysis of the extracts revealed the presence of alkaloids, triterpenes, steroids, and polyphenols, including flavonoids in most of the tested extracts. The entire tested extracts showed moderate (512 μg/mL ≤ MIC ≤ 2048 μg/mL) to weak (MIC > 2048 μg/mL) antibacterial activities against the tested bacteria. Furthermore, extracts of leaf of Tristemma hirtum and pericarps of Raphia hookeri (at their MIC/2 and MIC/4) strongly potentiated the activities of all antibiotics used in the study, especially those of chloramphenicol (CHL), ciprofloxacin (CIP), kanamycin (KAN), and tetracycline (TET) against 70% (7/10) to 100% (10/10) of the tested MDR bacteria, with the modulating factors ranging from 2 to 128. The results of this study suggest that extracts from leaves of Tristemma hirtum and pericarps of Raphia hookeri can be sources of plant-derived products with antibiotic modifying activity. |
| format | Article |
| id | doaj-art-d01f67dff10d402890a1d970a11f8b21 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d01f67dff10d402890a1d970a11f8b212025-02-03T06:00:21ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/76514827651482Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant PhenotypesGaëlle S. Nguenang0Armelle T. Mbaveng1Aimé G. Fankam2Hermione T. Manekeng3Paul Nayim4Brice E. N. Wamba5Victor Kuete6Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonDepartment of Biochemistry, Faculty of Science, University of Dschang, Dschang, CameroonIn order to contribute to the fight against infectious diseases, the in vitro antibacterial activity and the antibiotic-potentiating effects of Tristemma hirtum and five other Cameroonian edible plants have been evaluated against Gram-negative multidrug-resistant (MDR) phenotypes. The microdilution method was used to evaluate the bacterial susceptibility of the extracts and their combination to common antibiotics. The phytochemical screening of the extracts was carried out according to standard methods. Phytochemical analysis of the extracts revealed the presence of alkaloids, triterpenes, steroids, and polyphenols, including flavonoids in most of the tested extracts. The entire tested extracts showed moderate (512 μg/mL ≤ MIC ≤ 2048 μg/mL) to weak (MIC > 2048 μg/mL) antibacterial activities against the tested bacteria. Furthermore, extracts of leaf of Tristemma hirtum and pericarps of Raphia hookeri (at their MIC/2 and MIC/4) strongly potentiated the activities of all antibiotics used in the study, especially those of chloramphenicol (CHL), ciprofloxacin (CIP), kanamycin (KAN), and tetracycline (TET) against 70% (7/10) to 100% (10/10) of the tested MDR bacteria, with the modulating factors ranging from 2 to 128. The results of this study suggest that extracts from leaves of Tristemma hirtum and pericarps of Raphia hookeri can be sources of plant-derived products with antibiotic modifying activity.http://dx.doi.org/10.1155/2018/7651482 |
| spellingShingle | Gaëlle S. Nguenang Armelle T. Mbaveng Aimé G. Fankam Hermione T. Manekeng Paul Nayim Brice E. N. Wamba Victor Kuete Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes The Scientific World Journal |
| title | Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes |
| title_full | Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes |
| title_fullStr | Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes |
| title_full_unstemmed | Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes |
| title_short | Tristemma hirtum and Five Other Cameroonian Edible Plants with Weak or No Antibacterial Effects Modulate the Activities of Antibiotics against Gram-Negative Multidrug-Resistant Phenotypes |
| title_sort | tristemma hirtum and five other cameroonian edible plants with weak or no antibacterial effects modulate the activities of antibiotics against gram negative multidrug resistant phenotypes |
| url | http://dx.doi.org/10.1155/2018/7651482 |
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