MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway
Background The high invasion and heterogeneity of head and neck squamous cell carcinoma (HNSCC) commonly leads to poor clinical outcomes. Identification of reliable biomarkers for HNSCC is imperative.Methods The targeted gene with the highest mutation was screened out in cBioPortal database, and the...
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| Language: | English |
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Taylor & Francis Group
2024-01-01
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| Series: | Journal of Investigative Surgery |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/08941939.2024.2434096 |
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| author | Jing Liu Chaoyang Lv Sis Aghayants Yingying Wang |
| author_facet | Jing Liu Chaoyang Lv Sis Aghayants Yingying Wang |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Background The high invasion and heterogeneity of head and neck squamous cell carcinoma (HNSCC) commonly leads to poor clinical outcomes. Identification of reliable biomarkers for HNSCC is imperative.Methods The targeted gene with the highest mutation was screened out in cBioPortal database, and the interactive microRNAs (miRNAs) were identified by miRNA-mRNA co-expression analysis. CCK-8 and transwell assays were used to explore the proliferative, migrative, and invasive behaviors of HNSCC cells. The dual-luciferase reporter assay and cell transfection experiment were conducted. The role of miR-15a-5p was investigated in the in vivo xenograft mouse model.Results ATP binding cassette transporter 1 (ABCB1) had the highest mutation frequency and multiple mutation types in HNSCC, and the decreased ABCB1 was significantly related to better prognosis of HNSCC patients. MiR-15a-5p was a regulator for ABCB1, which was up-regulated after miR-15a-5p inhibition in vitro. Furthermore, the miR-15a-5p knockdown significantly suppressed HNSCC cell proliferation, migration, and invasion in vitro, and reduced the HNSCC tumor growth and migration capabilities in vivo, possibly through NF-κB signaling pathway.Conclusion Collectively, miR-15a-5p knockdown increased the ABCB1 level and abated the HNSCC progression via the NF-κB signaling pathway. ABCB1 and miR-15a-5p were underlying predictors for HNSCC therapeutic biomarkers. |
| format | Article |
| id | doaj-art-d01547b51fbd48e2b03133dd7741fc1d |
| institution | OA Journals |
| issn | 0894-1939 1521-0553 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Investigative Surgery |
| spelling | doaj-art-d01547b51fbd48e2b03133dd7741fc1d2025-08-20T01:57:39ZengTaylor & Francis GroupJournal of Investigative Surgery0894-19391521-05532024-01-0137110.1080/08941939.2024.2434096MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling PathwayJing Liu0Chaoyang Lv1Sis Aghayants2Yingying Wang3Outpatient Department, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Burn Plastic Wound Repair Surgery, Suizhou Hospital, Hubei University of Medicine, Suizhou, ChinaDepartment of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, ChinaBackground The high invasion and heterogeneity of head and neck squamous cell carcinoma (HNSCC) commonly leads to poor clinical outcomes. Identification of reliable biomarkers for HNSCC is imperative.Methods The targeted gene with the highest mutation was screened out in cBioPortal database, and the interactive microRNAs (miRNAs) were identified by miRNA-mRNA co-expression analysis. CCK-8 and transwell assays were used to explore the proliferative, migrative, and invasive behaviors of HNSCC cells. The dual-luciferase reporter assay and cell transfection experiment were conducted. The role of miR-15a-5p was investigated in the in vivo xenograft mouse model.Results ATP binding cassette transporter 1 (ABCB1) had the highest mutation frequency and multiple mutation types in HNSCC, and the decreased ABCB1 was significantly related to better prognosis of HNSCC patients. MiR-15a-5p was a regulator for ABCB1, which was up-regulated after miR-15a-5p inhibition in vitro. Furthermore, the miR-15a-5p knockdown significantly suppressed HNSCC cell proliferation, migration, and invasion in vitro, and reduced the HNSCC tumor growth and migration capabilities in vivo, possibly through NF-κB signaling pathway.Conclusion Collectively, miR-15a-5p knockdown increased the ABCB1 level and abated the HNSCC progression via the NF-κB signaling pathway. ABCB1 and miR-15a-5p were underlying predictors for HNSCC therapeutic biomarkers.https://www.tandfonline.com/doi/10.1080/08941939.2024.2434096HNSCCABCB1miR-15a-5pprognosisprogression |
| spellingShingle | Jing Liu Chaoyang Lv Sis Aghayants Yingying Wang MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway Journal of Investigative Surgery HNSCC ABCB1 miR-15a-5p prognosis progression |
| title | MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway |
| title_full | MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway |
| title_fullStr | MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway |
| title_full_unstemmed | MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway |
| title_short | MiR-15a-5p Knockdown up-Regulated ABCB1 Expression and Abated HNSCC Progression via the NF-κB Signaling Pathway |
| title_sort | mir 15a 5p knockdown up regulated abcb1 expression and abated hnscc progression via the nf κb signaling pathway |
| topic | HNSCC ABCB1 miR-15a-5p prognosis progression |
| url | https://www.tandfonline.com/doi/10.1080/08941939.2024.2434096 |
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