Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging
It has been reported that dysregulation of microRNA-155 expression and function is associated with tumorigenesis, growth, tumor subtypes, invasion, and poor survival rates. Peptide nucleic acid (PNA), an artificially synthesized nucleic acid mimic, has been applied for molecular diagnosis. In this s...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-06-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012120916124 |
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| author | Yaqun Jiang MD Yongkang Gai PhD Yu Long MD Qingyao Liu PhD Chunbao Liu MD, PhD Yongxue Zhang MD Xiaoli Lan MD, PhD |
| author_facet | Yaqun Jiang MD Yongkang Gai PhD Yu Long MD Qingyao Liu PhD Chunbao Liu MD, PhD Yongxue Zhang MD Xiaoli Lan MD, PhD |
| author_sort | Yaqun Jiang MD |
| collection | DOAJ |
| description | It has been reported that dysregulation of microRNA-155 expression and function is associated with tumorigenesis, growth, tumor subtypes, invasion, and poor survival rates. Peptide nucleic acid (PNA), an artificially synthesized nucleic acid mimic, has been applied for molecular diagnosis. In this study, a PNA sequence that undergoes complementary binding to miR-155 was labeled with 99m Tc to evaluate whether the tracer could visualize the expression of miR-155 in breast cancer. Both antisense PNA (anti-PNA, fully complementary bound to human mature miR-155 , referred to as “anti-PNA-155”) and mismatched PNA (referred to as “mis-PNA”) single strands containing 23-mer were synthesized. The relative expression of miR-155 in MCF-7 cells and tumors was higher than that in MDA-MB-231 cells and tumors. Single-photon emission computed tomography (SPECT) scan showed that radioactivity mainly accumulated in kidney. MCF-7 tumors, but not MDA-MB-231 tumors, were clearly visualized after [ 99m Tc]anti-PNA-155 injection. MCF-7 tumors were less visible when coinjected with 100-fold excess of anti-PNA-155 or injected with [ 99m Tc]mis-PNA, which suggested specific binding. Biodistribution study results were consistent with SPECT imaging. We successfully demonstrated that [ 99m Tc]anti-PNA-155 could visualize miR-155 expression in vivo, suggesting it may be a promising probe applied in breast cancer. |
| format | Article |
| id | doaj-art-d00ef43246ae43dbb9b808f01465e970 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-d00ef43246ae43dbb9b808f01465e9702025-02-03T10:07:51ZengSAGE PublishingMolecular Imaging1536-01212020-06-011910.1177/1536012120916124Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer ImagingYaqun Jiang MD0Yongkang Gai PhD1Yu Long MD2Qingyao Liu PhD3Chunbao Liu MD, PhD4Yongxue Zhang MD5Xiaoli Lan MD, PhD6 Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China Department of Nuclear Medicine, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Molecular Imaging, Wuhan, China Hubei Province Key Laboratory of Molecular Imaging, Wuhan, ChinaIt has been reported that dysregulation of microRNA-155 expression and function is associated with tumorigenesis, growth, tumor subtypes, invasion, and poor survival rates. Peptide nucleic acid (PNA), an artificially synthesized nucleic acid mimic, has been applied for molecular diagnosis. In this study, a PNA sequence that undergoes complementary binding to miR-155 was labeled with 99m Tc to evaluate whether the tracer could visualize the expression of miR-155 in breast cancer. Both antisense PNA (anti-PNA, fully complementary bound to human mature miR-155 , referred to as “anti-PNA-155”) and mismatched PNA (referred to as “mis-PNA”) single strands containing 23-mer were synthesized. The relative expression of miR-155 in MCF-7 cells and tumors was higher than that in MDA-MB-231 cells and tumors. Single-photon emission computed tomography (SPECT) scan showed that radioactivity mainly accumulated in kidney. MCF-7 tumors, but not MDA-MB-231 tumors, were clearly visualized after [ 99m Tc]anti-PNA-155 injection. MCF-7 tumors were less visible when coinjected with 100-fold excess of anti-PNA-155 or injected with [ 99m Tc]mis-PNA, which suggested specific binding. Biodistribution study results were consistent with SPECT imaging. We successfully demonstrated that [ 99m Tc]anti-PNA-155 could visualize miR-155 expression in vivo, suggesting it may be a promising probe applied in breast cancer.https://doi.org/10.1177/1536012120916124 |
| spellingShingle | Yaqun Jiang MD Yongkang Gai PhD Yu Long MD Qingyao Liu PhD Chunbao Liu MD, PhD Yongxue Zhang MD Xiaoli Lan MD, PhD Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging Molecular Imaging |
| title | Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging |
| title_full | Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging |
| title_fullStr | Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging |
| title_full_unstemmed | Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging |
| title_short | Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging |
| title_sort | application and evaluation of tc labeled peptide nucleic acid targeting in breast cancer imaging |
| url | https://doi.org/10.1177/1536012120916124 |
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