A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue
Tissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of gener...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Applied Bionics and Biomechanics |
| Online Access: | http://dx.doi.org/10.1155/2016/7465095 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556708599169024 |
|---|---|
| author | Emanuele Rizzuto Silvia Carosio Martina Faraldi Simona Pisu Antonio Musarò Zaccaria Del Prete |
| author_facet | Emanuele Rizzuto Silvia Carosio Martina Faraldi Simona Pisu Antonio Musarò Zaccaria Del Prete |
| author_sort | Emanuele Rizzuto |
| collection | DOAJ |
| description | Tissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of generating contractile forces is dependent on the internal fiber organization and is reflected by some macroscopic parameters, such as the spontaneous contraction. Here we propose the application of digital image correlation (DIC) as an independent tool for an accurate and noninvasive measurement of engineered muscle tissue spontaneous contraction. To validate the proposed technique we referred to the X-MET, a promising 3-dimensional model of skeletal muscle. The images acquired through a high speed camera were correlated with a custom-made algorithm and the longitudinal strain predictions were employed for measuring the spontaneous contraction. The spontaneous contraction reference values were obtained by studying the force response. The relative error between the spontaneous contraction frequencies computed in both ways was always lower than 0.15%. In conclusion, the use of a DIC based system allows for an accurate and noninvasive measurement of biological tissues’ spontaneous contraction, in addition to the measurement of tissue strain field on any desired region of interest during electrical stimulation. |
| format | Article |
| id | doaj-art-cff304d8b0b74562b2b4dee323f09a68 |
| institution | Kabale University |
| issn | 1176-2322 1754-2103 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied Bionics and Biomechanics |
| spelling | doaj-art-cff304d8b0b74562b2b4dee323f09a682025-02-03T05:44:36ZengWileyApplied Bionics and Biomechanics1176-23221754-21032016-01-01201610.1155/2016/74650957465095A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered TissueEmanuele Rizzuto0Silvia Carosio1Martina Faraldi2Simona Pisu3Antonio Musarò4Zaccaria Del Prete5Department of Mechanical and Aerospace Engineering, University of Rome La Sapienza, Via Eudossiana 18, 00184 Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, ItalyInstitute Pasteur Cenci-Bolognetti, Interuniversity Institute of Myology, Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences (DAHFMO), Unit of Histology and Medical Embryology, University of Rome La Sapienza, Via A. Scarpa 16, 00161 Rome, ItalyInstitute Pasteur Cenci-Bolognetti, Interuniversity Institute of Myology, Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences (DAHFMO), Unit of Histology and Medical Embryology, University of Rome La Sapienza, Via A. Scarpa 16, 00161 Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, ItalyDepartment of Mechanical and Aerospace Engineering, University of Rome La Sapienza, Via Eudossiana 18, 00184 Rome, ItalyTissue engineering is a multidisciplinary science based on the application of engineering approaches to biologic tissue formation. Engineered tissue internal organization represents a key aspect to increase biofunctionality before transplant and, as regarding skeletal muscles, the potential of generating contractile forces is dependent on the internal fiber organization and is reflected by some macroscopic parameters, such as the spontaneous contraction. Here we propose the application of digital image correlation (DIC) as an independent tool for an accurate and noninvasive measurement of engineered muscle tissue spontaneous contraction. To validate the proposed technique we referred to the X-MET, a promising 3-dimensional model of skeletal muscle. The images acquired through a high speed camera were correlated with a custom-made algorithm and the longitudinal strain predictions were employed for measuring the spontaneous contraction. The spontaneous contraction reference values were obtained by studying the force response. The relative error between the spontaneous contraction frequencies computed in both ways was always lower than 0.15%. In conclusion, the use of a DIC based system allows for an accurate and noninvasive measurement of biological tissues’ spontaneous contraction, in addition to the measurement of tissue strain field on any desired region of interest during electrical stimulation.http://dx.doi.org/10.1155/2016/7465095 |
| spellingShingle | Emanuele Rizzuto Silvia Carosio Martina Faraldi Simona Pisu Antonio Musarò Zaccaria Del Prete A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue Applied Bionics and Biomechanics |
| title | A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue |
| title_full | A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue |
| title_fullStr | A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue |
| title_full_unstemmed | A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue |
| title_short | A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue |
| title_sort | dic based technique to measure the contraction of a skeletal muscle engineered tissue |
| url | http://dx.doi.org/10.1155/2016/7465095 |
| work_keys_str_mv | AT emanuelerizzuto adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT silviacarosio adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT martinafaraldi adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT simonapisu adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT antoniomusaro adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT zaccariadelprete adicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT emanuelerizzuto dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT silviacarosio dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT martinafaraldi dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT simonapisu dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT antoniomusaro dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue AT zaccariadelprete dicbasedtechniquetomeasurethecontractionofaskeletalmuscleengineeredtissue |