EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT

Considering both the health and energy areas where anthocyanins have been revealing their real and potential applications, research on their extraction optimization methods is a priority. Conditions to achieve the highest mass of crude extract from Tradescantia spathacea leaves with the highest abso...

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Main Authors: ANILÚ MIRANDA-MEDINA, PAOLA LIBERTAD GARCÍA-MEDEL, KAREN RODRÍGUEZ-MARTÍNEZ, PATRICIA MARGARET HAYWARD-JONES, DULCE MARÍA BARRADAS-DERMITZ, GEORGINA LUNA-CARRILLO
Format: Article
Language:English
Published: Alma Mater Publishing House "Vasile Alecsandri" University of Bacau 2023-03-01
Series:Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
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Online Access:https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202301&vol=1&aid=5528
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author ANILÚ MIRANDA-MEDINA
PAOLA LIBERTAD GARCÍA-MEDEL
KAREN RODRÍGUEZ-MARTÍNEZ
PATRICIA MARGARET HAYWARD-JONES
DULCE MARÍA BARRADAS-DERMITZ
GEORGINA LUNA-CARRILLO
author_facet ANILÚ MIRANDA-MEDINA
PAOLA LIBERTAD GARCÍA-MEDEL
KAREN RODRÍGUEZ-MARTÍNEZ
PATRICIA MARGARET HAYWARD-JONES
DULCE MARÍA BARRADAS-DERMITZ
GEORGINA LUNA-CARRILLO
author_sort ANILÚ MIRANDA-MEDINA
collection DOAJ
description Considering both the health and energy areas where anthocyanins have been revealing their real and potential applications, research on their extraction optimization methods is a priority. Conditions to achieve the highest mass of crude extract from Tradescantia spathacea leaves with the highest absorbance (A) in the region of 500 - 540 nm linked with anthocyanins, were investigated through a Box-Behnken design and response surface methodology. Independent variables involved in the Box-Behnken design were solvent (ethanol) concentration (30, 50, 70 % v/v), solvent/fresh leaf ratio (1.67:1, 2.34:1; 3:1 v/w) and extraction temperature (30, 50, 70 °C). Dependent (response) variables were crude extract amount and the extract with the maximum A in the region of 500-540 nm. Experimental values of A 500 - 540 nm were 0.3562 and 0.7558; crude extract mass ranged between 0.285 g and 0.7157 g, corresponding to a yield between 144 to 347 mg dried extract per g of dried leaf. Using a solvent/fresh leaf ratio of 3:1 v/w, 70 °C, 65 % ethanol, the highest values of both crude extract mass and A 500 - 540 nm were obtained.
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publisher Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
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spelling doaj-art-cfa525c39dee4256ae3d56f3ad10d6b12025-08-20T02:20:07ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X2023-03-01241001015EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENTANILÚ MIRANDA-MEDINA 0 PAOLA LIBERTAD GARCÍA-MEDEL 1KAREN RODRÍGUEZ-MARTÍNEZ 2PATRICIA MARGARET HAYWARD-JONES 3DULCE MARÍA BARRADAS-DERMITZ 4GEORGINA LUNA-CARRILLO 5National Institute of Technology of Mexico-Veracruz Institute of Technology campus: 1. Chemical and Biochemical Engineering DepartmentNational Institute of Technology of Mexico-Veracruz Institute of Technology campus: 1. Chemical and Biochemical Engineering DepartmentNational Institute of Technology of Mexico-Veracruz Institute of Technology campus: 1. Chemical and Biochemical Engineering DepartmentNational Institute of Technology of Mexico-Veracruz Institute of Technology campus: 2. Biological-Chemistry AreaNational Institute of Technology of Mexico-Veracruz Institute of Technology campus: 2. Biological-Chemistry AreaNational Institute of Technology of Mexico-Veracruz Institute of Technology campus: 1. Chemical and Biochemical Engineering DepartmentConsidering both the health and energy areas where anthocyanins have been revealing their real and potential applications, research on their extraction optimization methods is a priority. Conditions to achieve the highest mass of crude extract from Tradescantia spathacea leaves with the highest absorbance (A) in the region of 500 - 540 nm linked with anthocyanins, were investigated through a Box-Behnken design and response surface methodology. Independent variables involved in the Box-Behnken design were solvent (ethanol) concentration (30, 50, 70 % v/v), solvent/fresh leaf ratio (1.67:1, 2.34:1; 3:1 v/w) and extraction temperature (30, 50, 70 °C). Dependent (response) variables were crude extract amount and the extract with the maximum A in the region of 500-540 nm. Experimental values of A 500 - 540 nm were 0.3562 and 0.7558; crude extract mass ranged between 0.285 g and 0.7157 g, corresponding to a yield between 144 to 347 mg dried extract per g of dried leaf. Using a solvent/fresh leaf ratio of 3:1 v/w, 70 °C, 65 % ethanol, the highest values of both crude extract mass and A 500 - 540 nm were obtained.https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202301&vol=1&aid=5528extractionhealth and energy applicationsnatural red pigments
spellingShingle ANILÚ MIRANDA-MEDINA
PAOLA LIBERTAD GARCÍA-MEDEL
KAREN RODRÍGUEZ-MARTÍNEZ
PATRICIA MARGARET HAYWARD-JONES
DULCE MARÍA BARRADAS-DERMITZ
GEORGINA LUNA-CARRILLO
EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
extraction
health and energy applications
natural red pigments
title EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
title_full EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
title_fullStr EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
title_full_unstemmed EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
title_short EXTRACTION OPTIMIZATION OF TRADESCANTIA SPATHACEA SW. LEAF CRUDE EXTRACT AND ANTHOCYANIN CONTENT
title_sort extraction optimization of tradescantia spathacea sw leaf crude extract and anthocyanin content
topic extraction
health and energy applications
natural red pigments
url https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202301&vol=1&aid=5528
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