Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activit...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/692392 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559242983243776 |
|---|---|
| author | Richa Upadhyay Jitendra Kumar Chaurasia Kavindra Nath Tiwari Karuna Singh |
| author_facet | Richa Upadhyay Jitendra Kumar Chaurasia Kavindra Nath Tiwari Karuna Singh |
| author_sort | Richa Upadhyay |
| collection | DOAJ |
| description | In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50 of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe3+-Fe2+ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant. |
| format | Article |
| id | doaj-art-cf9ff9b2521f4fab9f2038de51d91514 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cf9ff9b2521f4fab9f2038de51d915142025-02-03T01:30:33ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/692392692392Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal PlantRicha Upadhyay0Jitendra Kumar Chaurasia1Kavindra Nath Tiwari2Karuna Singh3Department of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDepartment of Applied Science, Kashi Institute of Technology, Varanasi, Uttar Pradesh 221307, IndiaDepartment of Botany, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaDepartment of Zoology, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, IndiaIn present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with EC50 of aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe3+-Fe2+ transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant.http://dx.doi.org/10.1155/2014/692392 |
| spellingShingle | Richa Upadhyay Jitendra Kumar Chaurasia Kavindra Nath Tiwari Karuna Singh Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant The Scientific World Journal |
| title | Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant |
| title_full | Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant |
| title_fullStr | Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant |
| title_full_unstemmed | Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant |
| title_short | Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant |
| title_sort | antioxidant property of aerial parts and root of phyllanthus fraternus webster an important medicinal plant |
| url | http://dx.doi.org/10.1155/2014/692392 |
| work_keys_str_mv | AT richaupadhyay antioxidantpropertyofaerialpartsandrootofphyllanthusfraternuswebsteranimportantmedicinalplant AT jitendrakumarchaurasia antioxidantpropertyofaerialpartsandrootofphyllanthusfraternuswebsteranimportantmedicinalplant AT kavindranathtiwari antioxidantpropertyofaerialpartsandrootofphyllanthusfraternuswebsteranimportantmedicinalplant AT karunasingh antioxidantpropertyofaerialpartsandrootofphyllanthusfraternuswebsteranimportantmedicinalplant |