P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors
Background. Besides its major role in cell proliferation, DNA repair, and apoptosis, functional p53 protein is involved in the induction of antitumor cytotoxic-T-cell activity against carcinoma cells. We aimed to investigate p53 and immune cell markers utility as diagnostic and prognostic markers of...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/846584 |
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| author | Marjory Alana Marcello Elaine Cristina Morari Lucas Leite Cunha Aline Carolina De Nadai Silva Dirce Maria Carraro André Lopes Carvalho Fernando Augusto Soares José Vassallo Laura Sterian Ward |
| author_facet | Marjory Alana Marcello Elaine Cristina Morari Lucas Leite Cunha Aline Carolina De Nadai Silva Dirce Maria Carraro André Lopes Carvalho Fernando Augusto Soares José Vassallo Laura Sterian Ward |
| author_sort | Marjory Alana Marcello |
| collection | DOAJ |
| description | Background. Besides its major role in cell proliferation, DNA repair, and apoptosis, functional p53 protein is involved in the induction of antitumor cytotoxic-T-cell activity against carcinoma cells. We aimed to investigate p53 and immune cell markers utility as diagnostic and prognostic markers of differentiated thyroid cancer (DTC). Methods. ACIS-III system was used to evaluate p53 and immune cell markers including tumor-associated macrophages (TAM); CD68 and tumor-infiltrating lymphocytes (TIL) subsets such as CD3, CD4, CD8, and CD20 in 206 thyroid carcinomas, 105 benign nodules, and 18 normal tissues. Also, TP53 was sequenced in 78 out of 164 patients with papillary thyroid carcinoma. Results. P53 expression was observed more frequently in malignant than in benign lesions (P<0.0001) and helped discriminate follicular patterned lesions. In addition, p53 was more frequent in smaller (P=0.0015), unique tumors (P=0.0286), with thyroiditis (P=0.0486) and without metastasis at diagnosis (P=0.0201). TAM was more frequent in P53 negative tumors (P=0.002). Infiltration of CD8+ TIL was found in 61.7% of P53 positive and 25.6% of P53 negative DTC (P<0.001). Conclusions. We suggest that p53 and CD8+ TIL immune profile analysis might be useful in DTC. |
| format | Article |
| id | doaj-art-cf91b8871cea4fb18ba0a61cfb788e8f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-cf91b8871cea4fb18ba0a61cfb788e8f2025-02-03T05:58:39ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/846584846584P53 and Expression of Immunological Markers May Identify Early Stage Thyroid TumorsMarjory Alana Marcello0Elaine Cristina Morari1Lucas Leite Cunha2Aline Carolina De Nadai Silva3Dirce Maria Carraro4André Lopes Carvalho5Fernando Augusto Soares6José Vassallo7Laura Sterian Ward8Laboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), Rua Tessalia Vieira de Camargo 126, Cidade Universitaria Zeferino Vaz, Campinas 13083-887, SP, BrazilLaboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), Rua Tessalia Vieira de Camargo 126, Cidade Universitaria Zeferino Vaz, Campinas 13083-887, SP, BrazilLaboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), Rua Tessalia Vieira de Camargo 126, Cidade Universitaria Zeferino Vaz, Campinas 13083-887, SP, BrazilLaboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), Rua Tessalia Vieira de Camargo 126, Cidade Universitaria Zeferino Vaz, Campinas 13083-887, SP, BrazilLaboratory of Genomics and Molecular Biology, CIPE, AC Camargo Hospital, Rua Tagua 440, 1º andar, São Paulo, SP, BrazilDepartment of Head and Neck Surgery, Barretos Cancer Hospital, Avenida Antenor Duarte Villela 1331, Barretos, SP, BrazilDepartment of Pathology, AC Camargo Cancer Center, Rua Prof. Antônio Prudente 109, 1º andar, Anatomia Patológica, São Paulo, SP, BrazilDepartment of Pathology, AC Camargo Cancer Center, Rua Prof. Antônio Prudente 109, 1º andar, Anatomia Patológica, São Paulo, SP, BrazilLaboratory of Cancer Molecular Genetics, Faculty of Medical Sciences (FCM), University of Campinas (Unicamp), Rua Tessalia Vieira de Camargo 126, Cidade Universitaria Zeferino Vaz, Campinas 13083-887, SP, BrazilBackground. Besides its major role in cell proliferation, DNA repair, and apoptosis, functional p53 protein is involved in the induction of antitumor cytotoxic-T-cell activity against carcinoma cells. We aimed to investigate p53 and immune cell markers utility as diagnostic and prognostic markers of differentiated thyroid cancer (DTC). Methods. ACIS-III system was used to evaluate p53 and immune cell markers including tumor-associated macrophages (TAM); CD68 and tumor-infiltrating lymphocytes (TIL) subsets such as CD3, CD4, CD8, and CD20 in 206 thyroid carcinomas, 105 benign nodules, and 18 normal tissues. Also, TP53 was sequenced in 78 out of 164 patients with papillary thyroid carcinoma. Results. P53 expression was observed more frequently in malignant than in benign lesions (P<0.0001) and helped discriminate follicular patterned lesions. In addition, p53 was more frequent in smaller (P=0.0015), unique tumors (P=0.0286), with thyroiditis (P=0.0486) and without metastasis at diagnosis (P=0.0201). TAM was more frequent in P53 negative tumors (P=0.002). Infiltration of CD8+ TIL was found in 61.7% of P53 positive and 25.6% of P53 negative DTC (P<0.001). Conclusions. We suggest that p53 and CD8+ TIL immune profile analysis might be useful in DTC.http://dx.doi.org/10.1155/2013/846584 |
| spellingShingle | Marjory Alana Marcello Elaine Cristina Morari Lucas Leite Cunha Aline Carolina De Nadai Silva Dirce Maria Carraro André Lopes Carvalho Fernando Augusto Soares José Vassallo Laura Sterian Ward P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors Clinical and Developmental Immunology |
| title | P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors |
| title_full | P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors |
| title_fullStr | P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors |
| title_full_unstemmed | P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors |
| title_short | P53 and Expression of Immunological Markers May Identify Early Stage Thyroid Tumors |
| title_sort | p53 and expression of immunological markers may identify early stage thyroid tumors |
| url | http://dx.doi.org/10.1155/2013/846584 |
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