Separation of Monocytes from Whole Human Blood
Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The m...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.842 |
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| _version_ | 1832567197287841792 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats. |
| format | Article |
| id | doaj-art-cf78269e11f44c45b2477882ad894703 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cf78269e11f44c45b2477882ad8947032025-02-03T01:02:15ZengWileyThe Scientific World Journal1537-744X2002-01-0121540154310.1100/tsw.2002.842Separation of Monocytes from Whole Human BloodJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKHuman peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats.http://dx.doi.org/10.1100/tsw.2002.842 |
| spellingShingle | John Graham Separation of Monocytes from Whole Human Blood The Scientific World Journal |
| title | Separation of Monocytes from Whole Human Blood |
| title_full | Separation of Monocytes from Whole Human Blood |
| title_fullStr | Separation of Monocytes from Whole Human Blood |
| title_full_unstemmed | Separation of Monocytes from Whole Human Blood |
| title_short | Separation of Monocytes from Whole Human Blood |
| title_sort | separation of monocytes from whole human blood |
| url | http://dx.doi.org/10.1100/tsw.2002.842 |
| work_keys_str_mv | AT johngraham separationofmonocytesfromwholehumanblood |