Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells
Triptolide, the major component of Chinese herbal medicine Tripterygium wilfordii Hook F, possesses potent anticancer and anti-inflammatory effects. IL-8, a proinflammatory cytokine, is associated with cancer cell proliferation and angiogenesis. Here, we found that Triptolide has an inhibitory effec...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1498213/full |
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| author | Shinan Li Shinan Li Dhiraj Kumar Sah Archana Arjunan Mohamed Yazeer Ameer Bora Lee Young-Do Jung |
| author_facet | Shinan Li Shinan Li Dhiraj Kumar Sah Archana Arjunan Mohamed Yazeer Ameer Bora Lee Young-Do Jung |
| author_sort | Shinan Li |
| collection | DOAJ |
| description | Triptolide, the major component of Chinese herbal medicine Tripterygium wilfordii Hook F, possesses potent anticancer and anti-inflammatory effects. IL-8, a proinflammatory cytokine, is associated with cancer cell proliferation and angiogenesis. Here, we found that Triptolide has an inhibitory effect on IL-1β-induced IL-8 expression in human gastric cancer cells, via the suppression of reactive oxygen species (ROS) production, AP-1, and NF-κB activation, which in turn affects human endothelial cell angiogenetic activity in tumor microenvironments. Human gastric AGS cells were treated with IL-1β (10 ng/mL) and Triptolide (0–20 nM), and the ROS generation, ERK, AP-1, and NF-κB signaling were all investigated. These results demonstrate that Triptolide inhibits the IL-1β-induced IL-8 expression in gastric cancer cells by inhibiting ROS production and angiogenesis, via the dose-dependent attenuation of ERK, AP-1, and NF-κB activation. In this study, we showed that Triptolid inhibits ROS/ERK-mediated AP-1 and ROS-mediated NF-κB axes potentially leading to an improved treatment outcome for gastric cancer and its associated tumor microenvironment. |
| format | Article |
| id | doaj-art-cf6ebcb125d7485cb81830d911c74f27 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Oncology |
| spelling | doaj-art-cf6ebcb125d7485cb81830d911c74f272025-01-30T05:10:18ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-01-011410.3389/fonc.2024.14982131498213Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cellsShinan Li0Shinan Li1Dhiraj Kumar Sah2Archana Arjunan3Mohamed Yazeer Ameer4Bora Lee5Young-Do Jung6Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Medical University, Taiyuan, ChinaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Hwasun, Republic of KoreaTriptolide, the major component of Chinese herbal medicine Tripterygium wilfordii Hook F, possesses potent anticancer and anti-inflammatory effects. IL-8, a proinflammatory cytokine, is associated with cancer cell proliferation and angiogenesis. Here, we found that Triptolide has an inhibitory effect on IL-1β-induced IL-8 expression in human gastric cancer cells, via the suppression of reactive oxygen species (ROS) production, AP-1, and NF-κB activation, which in turn affects human endothelial cell angiogenetic activity in tumor microenvironments. Human gastric AGS cells were treated with IL-1β (10 ng/mL) and Triptolide (0–20 nM), and the ROS generation, ERK, AP-1, and NF-κB signaling were all investigated. These results demonstrate that Triptolide inhibits the IL-1β-induced IL-8 expression in gastric cancer cells by inhibiting ROS production and angiogenesis, via the dose-dependent attenuation of ERK, AP-1, and NF-κB activation. In this study, we showed that Triptolid inhibits ROS/ERK-mediated AP-1 and ROS-mediated NF-κB axes potentially leading to an improved treatment outcome for gastric cancer and its associated tumor microenvironment.https://www.frontiersin.org/articles/10.3389/fonc.2024.1498213/fullangiogenesisERK1/2gastric cancerInterleukin-1ßreactive oxygen speciestriptolide |
| spellingShingle | Shinan Li Shinan Li Dhiraj Kumar Sah Archana Arjunan Mohamed Yazeer Ameer Bora Lee Young-Do Jung Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells Frontiers in Oncology angiogenesis ERK1/2 gastric cancer Interleukin-1ß reactive oxygen species triptolide |
| title | Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells |
| title_full | Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells |
| title_fullStr | Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells |
| title_full_unstemmed | Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells |
| title_short | Triptolide suppresses IL-1β-induced expression of interleukin-8 by inhibiting ROS-Mediated ERK, AP-1, and NF-κB molecules in human gastric cancer AGS cells |
| title_sort | triptolide suppresses il 1β induced expression of interleukin 8 by inhibiting ros mediated erk ap 1 and nf κb molecules in human gastric cancer ags cells |
| topic | angiogenesis ERK1/2 gastric cancer Interleukin-1ß reactive oxygen species triptolide |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1498213/full |
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