Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry
This paper was conducted to develop a method containing ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for simultaneous quantification of six bioactive components in the decoction of Ziziphi spinosae Semen. Analysis was performed on an Agilent ZOR...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8397818 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556637109354496 |
|---|---|
| author | Xiao Liu Xiaochai Zhu Hui Zhu Li Xie Jia Ma Yinghui Xu Qigang Zhou Zejun Wu Baochang Cai |
| author_facet | Xiao Liu Xiaochai Zhu Hui Zhu Li Xie Jia Ma Yinghui Xu Qigang Zhou Zejun Wu Baochang Cai |
| author_sort | Xiao Liu |
| collection | DOAJ |
| description | This paper was conducted to develop a method containing ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for simultaneous quantification of six bioactive components in the decoction of Ziziphi spinosae Semen. Analysis was performed on an Agilent ZORBAX Extend-C18 column (2.1 × 100 mm, 1.8 μm) and eluted with a mobile phase system consisting of acetonitrile and water under a gradient program with a flow rate of 0.3 ml/min. The injection volume was 2 μl. Multiple-reaction monitoring scanning detection was employed for quantification with an electrospray ion source in the negative ion mode. All the six compounds showed good linearities (r≥0.9996). The LODs of the six bioactive compounds were 0.039 ng/ml, 0.092 ng/ml, 3.112 ng/ml, 2.131 ng/ml, 0.099 ng/ml, and 0.071 ng/ml for spinosin, 6‴-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. The LOQs were 0.118 ng/ml, 0.276 ng/ml, 9.336 ng/ml, 6.393 ng/ml, 0.299 ng/ml, and 0.213 ng/ml for spinosin, 6‴-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. According to our knowledge, it was the first time to establish a method with high efficiency and accuracy for the quantification of six bioactive components in the decoction of Ziziphi spinosae Semen, which would provide references for quality control and evaluation of Ziziphi spinosae Semen. |
| format | Article |
| id | doaj-art-cf56b3cf663d4172b20e404203502cc2 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-cf56b3cf663d4172b20e404203502cc22025-02-03T05:44:50ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/83978188397818Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass SpectrometryXiao Liu0Xiaochai Zhu1Hui Zhu2Li Xie3Jia Ma4Yinghui Xu5Qigang Zhou6Zejun Wu7Baochang Cai8School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaFushun Central Hospital, Fushun, ChinaFushun Central Hospital, Fushun, ChinaDepartment of Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaThis paper was conducted to develop a method containing ultrahigh performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for simultaneous quantification of six bioactive components in the decoction of Ziziphi spinosae Semen. Analysis was performed on an Agilent ZORBAX Extend-C18 column (2.1 × 100 mm, 1.8 μm) and eluted with a mobile phase system consisting of acetonitrile and water under a gradient program with a flow rate of 0.3 ml/min. The injection volume was 2 μl. Multiple-reaction monitoring scanning detection was employed for quantification with an electrospray ion source in the negative ion mode. All the six compounds showed good linearities (r≥0.9996). The LODs of the six bioactive compounds were 0.039 ng/ml, 0.092 ng/ml, 3.112 ng/ml, 2.131 ng/ml, 0.099 ng/ml, and 0.071 ng/ml for spinosin, 6‴-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. The LOQs were 0.118 ng/ml, 0.276 ng/ml, 9.336 ng/ml, 6.393 ng/ml, 0.299 ng/ml, and 0.213 ng/ml for spinosin, 6‴-feruloylspinosin, jujuboside A, jujuboside B, camelliaside B, and betulinic acid, respectively. According to our knowledge, it was the first time to establish a method with high efficiency and accuracy for the quantification of six bioactive components in the decoction of Ziziphi spinosae Semen, which would provide references for quality control and evaluation of Ziziphi spinosae Semen.http://dx.doi.org/10.1155/2018/8397818 |
| spellingShingle | Xiao Liu Xiaochai Zhu Hui Zhu Li Xie Jia Ma Yinghui Xu Qigang Zhou Zejun Wu Baochang Cai Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
| title_full | Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
| title_fullStr | Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
| title_full_unstemmed | Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
| title_short | Simultaneous Quantification of Six Bioactive Components in Decoction of Ziziphi spinosae Semen Using Ultrahigh Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry |
| title_sort | simultaneous quantification of six bioactive components in decoction of ziziphi spinosae semen using ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry |
| url | http://dx.doi.org/10.1155/2018/8397818 |
| work_keys_str_mv | AT xiaoliu simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT xiaochaizhu simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT huizhu simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT lixie simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT jiama simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT yinghuixu simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT qigangzhou simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT zejunwu simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry AT baochangcai simultaneousquantificationofsixbioactivecomponentsindecoctionofziziphispinosaesemenusingultrahighperformanceliquidchromatographycoupledwithtriplequadrupolemassspectrometry |