Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats
Aplastic anemia (AA) is a common malignant blood disease, and chronic intermittent hypobaric hypoxia (CIHH) has a beneficial effect against different diseases. The aim of the present study was to investigate the protective effect of CIHH against AA and underlying mechanisms. 5-Fluorouracil and busul...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/5706193 |
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| author | Jing Yang Li Zhang Handong Wang Zan Guo Yixian Liu Yi Zhang Chuan Wang Quanhai Li |
| author_facet | Jing Yang Li Zhang Handong Wang Zan Guo Yixian Liu Yi Zhang Chuan Wang Quanhai Li |
| author_sort | Jing Yang |
| collection | DOAJ |
| description | Aplastic anemia (AA) is a common malignant blood disease, and chronic intermittent hypobaric hypoxia (CIHH) has a beneficial effect against different diseases. The aim of the present study was to investigate the protective effect of CIHH against AA and underlying mechanisms. 5-Fluorouracil and busulfan treatment induced AA model in rats with reduction of hematological parameters and bone marrow tissue injury and decrease of the colony numbers of progenitor cells. CIHH pretreatment significantly reduced the incidence rate of AA and alleviated above symptoms in AA model. The adhesive molecules of bone marrow mesenchymal stem cells (BMMSCs) in AA model, VLA-4, VCAM-1, and ICAM-1 were upregulated, and those of CD162 and CD164 were downregulated by CIHH pretreatment. The expressions of HIF-1α and NF-κB in BMMSCs were also decreased through CIHH pretreatment. Overall, the results demonstrated for the first time that CIHH has an anti-AA effect through improving the adhesiveness and stress of mesenchymal stem cells in rats. CIHH could be a promising and effective therapy for AA. |
| format | Article |
| id | doaj-art-cf4fe571b4514e58ba93ad14f2179eae |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-cf4fe571b4514e58ba93ad14f2179eae2025-02-03T01:22:20ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/57061935706193Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in RatsJing Yang0Li Zhang1Handong Wang2Zan Guo3Yixian Liu4Yi Zhang5Chuan Wang6Quanhai Li7Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaDepartment of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaUndergraduate of College of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaDepartment of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaDepartment of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaDepartment of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaDepartment of Pharmacology, Hebei Medical University, Shijiazhuang, Hebei 050017, ChinaCell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, ChinaAplastic anemia (AA) is a common malignant blood disease, and chronic intermittent hypobaric hypoxia (CIHH) has a beneficial effect against different diseases. The aim of the present study was to investigate the protective effect of CIHH against AA and underlying mechanisms. 5-Fluorouracil and busulfan treatment induced AA model in rats with reduction of hematological parameters and bone marrow tissue injury and decrease of the colony numbers of progenitor cells. CIHH pretreatment significantly reduced the incidence rate of AA and alleviated above symptoms in AA model. The adhesive molecules of bone marrow mesenchymal stem cells (BMMSCs) in AA model, VLA-4, VCAM-1, and ICAM-1 were upregulated, and those of CD162 and CD164 were downregulated by CIHH pretreatment. The expressions of HIF-1α and NF-κB in BMMSCs were also decreased through CIHH pretreatment. Overall, the results demonstrated for the first time that CIHH has an anti-AA effect through improving the adhesiveness and stress of mesenchymal stem cells in rats. CIHH could be a promising and effective therapy for AA.http://dx.doi.org/10.1155/2017/5706193 |
| spellingShingle | Jing Yang Li Zhang Handong Wang Zan Guo Yixian Liu Yi Zhang Chuan Wang Quanhai Li Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats Stem Cells International |
| title | Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats |
| title_full | Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats |
| title_fullStr | Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats |
| title_full_unstemmed | Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats |
| title_short | Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats |
| title_sort | protective effects of chronic intermittent hypobaric hypoxia pretreatment against aplastic anemia through improving the adhesiveness and stress of mesenchymal stem cells in rats |
| url | http://dx.doi.org/10.1155/2017/5706193 |
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