Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
The role of different receptors in natural-killer- (NK-) cell-mediated cytotoxicity against multiple myeloma (MM) cells is unknown. We investigated if an enhancement of NK-cell-mediated cytotoxicity against MM could be reached by blocking of the inhibitory leukocyte immunoglobulin-like receptor 1 (L...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/652130 |
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| author | Silke Heidenreich Christine zu Eulenburg York Hildebrandt Thomas Stübig Heidi Sierich Anita Badbaran Thomas H. Eiermann Thomas M. C. Binder Nicolaus Kröger |
| author_facet | Silke Heidenreich Christine zu Eulenburg York Hildebrandt Thomas Stübig Heidi Sierich Anita Badbaran Thomas H. Eiermann Thomas M. C. Binder Nicolaus Kröger |
| author_sort | Silke Heidenreich |
| collection | DOAJ |
| description | The role of different receptors in natural-killer- (NK-) cell-mediated cytotoxicity against multiple myeloma (MM) cells is unknown. We investigated if an enhancement of NK-cell-mediated cytotoxicity against MM could be reached by blocking of the inhibitory leukocyte immunoglobulin-like receptor 1 (LIR-1). Our investigations revealed high levels of LIR-1 expression not only on the NK cell line NK-92, but also on myeloma cells (MOLP-8, RPMI8226) as well as on a lymphoblastoid cell line (LBCL; IM-9). Subsequent cytotoxicity assays were designed to show the isolated effects of LIR-1 blocking on either the effector or the tumor side to rule out receptor-receptor interactions. Although NK-92 was shown to be capable of myeloma cell lysis, inhibition of LIR-1 on NK-92 did not enhance cytotoxicity. Targeting the receptor on MM and LBCL did not also alter NK-92-mediated lysis. We come to the conclusion that LIR-1 alone does not directly influence NK-cell-mediated cytotoxicity against myeloma. To our knowledge, this work provides the first investigation of the inhibitory capability of LIR-1 in NK-92-mediated cytotoxicity against MM and the first functional evaluation of LIR-1 on MM and LBCL. |
| format | Article |
| id | doaj-art-cf4a52680c1e4abfaecacfaadee406d5 |
| institution | DOAJ |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-cf4a52680c1e4abfaecacfaadee406d52025-08-20T03:23:42ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/652130652130Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple MyelomaSilke Heidenreich0Christine zu Eulenburg1York Hildebrandt2Thomas Stübig3Heidi Sierich4Anita Badbaran5Thomas H. Eiermann6Thomas M. C. Binder7Nicolaus Kröger8Clinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, GermanyDepartment of Medical Biometry and Epidemiology, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyClinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, GermanyClinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, GermanyDepartment of Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyClinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, GermanyDepartment of Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyDepartment of Transfusion Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, GermanyClinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, GermanyThe role of different receptors in natural-killer- (NK-) cell-mediated cytotoxicity against multiple myeloma (MM) cells is unknown. We investigated if an enhancement of NK-cell-mediated cytotoxicity against MM could be reached by blocking of the inhibitory leukocyte immunoglobulin-like receptor 1 (LIR-1). Our investigations revealed high levels of LIR-1 expression not only on the NK cell line NK-92, but also on myeloma cells (MOLP-8, RPMI8226) as well as on a lymphoblastoid cell line (LBCL; IM-9). Subsequent cytotoxicity assays were designed to show the isolated effects of LIR-1 blocking on either the effector or the tumor side to rule out receptor-receptor interactions. Although NK-92 was shown to be capable of myeloma cell lysis, inhibition of LIR-1 on NK-92 did not enhance cytotoxicity. Targeting the receptor on MM and LBCL did not also alter NK-92-mediated lysis. We come to the conclusion that LIR-1 alone does not directly influence NK-cell-mediated cytotoxicity against myeloma. To our knowledge, this work provides the first investigation of the inhibitory capability of LIR-1 in NK-92-mediated cytotoxicity against MM and the first functional evaluation of LIR-1 on MM and LBCL.http://dx.doi.org/10.1155/2012/652130 |
| spellingShingle | Silke Heidenreich Christine zu Eulenburg York Hildebrandt Thomas Stübig Heidi Sierich Anita Badbaran Thomas H. Eiermann Thomas M. C. Binder Nicolaus Kröger Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma Clinical and Developmental Immunology |
| title | Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma |
| title_full | Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma |
| title_fullStr | Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma |
| title_full_unstemmed | Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma |
| title_short | Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma |
| title_sort | impact of the nk cell receptor lir 1 ilt 2 cd85j lilrb1 on cytotoxicity against multiple myeloma |
| url | http://dx.doi.org/10.1155/2012/652130 |
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