Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function.
T-cell response plays an important role in SARS-CoV-2 immunogenicity. For people living with HIV (PWH) and solid organ transplant (SOT) recipients there is limited evidence on the reliability of commercially available T-cell tests. We assessed 173 blood samples from 81 participants (62 samples from...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0317965 |
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| author | Annette Audigé Alain Amstutz Macé M Schuurmans Patrizia Amico Dominique L Braun Marcel P Stoeckle Barbara Hasse René Hage Dominik Damm Michael Tamm Nicolas J Mueller Huldrych F Günthard Michael T Koller Christof M Schönenberger Alexandra Griessbach Niklaus D Labhardt Roger D Kouyos Alexandra Trkola Michael Huber Katharina Kusejko Heiner C Bucher Irene A Abela Matthias Briel Frédérique Chammartin Benjamin Speich Swiss HIV Cohort Study, and the Swiss Transplant Cohort Study |
| author_facet | Annette Audigé Alain Amstutz Macé M Schuurmans Patrizia Amico Dominique L Braun Marcel P Stoeckle Barbara Hasse René Hage Dominik Damm Michael Tamm Nicolas J Mueller Huldrych F Günthard Michael T Koller Christof M Schönenberger Alexandra Griessbach Niklaus D Labhardt Roger D Kouyos Alexandra Trkola Michael Huber Katharina Kusejko Heiner C Bucher Irene A Abela Matthias Briel Frédérique Chammartin Benjamin Speich Swiss HIV Cohort Study, and the Swiss Transplant Cohort Study |
| author_sort | Annette Audigé |
| collection | DOAJ |
| description | T-cell response plays an important role in SARS-CoV-2 immunogenicity. For people living with HIV (PWH) and solid organ transplant (SOT) recipients there is limited evidence on the reliability of commercially available T-cell tests. We assessed 173 blood samples from 81 participants (62 samples from 35 PWH; 111 samples from 46 SOT recipients [lung and kidney]) with two commercial SARS-CoV-2 Interferon-γ (IFN-γ) release assays (IGRA; SARS-CoV-2 IGRA by Euroimmun, and IGRA SARS-CoV-2 by Roche). The reliability between the tests was judged as low (Cohen's kappa [κ] = 0.20; overall percent agreement [OPA] = 66%). A high proportion of tests were invalid (22% Euroimmun; 8% Roche). When excluding these invalid tests, the agreement was higher (κ = 0.43; OPA = 90%). The low reliability between the two T-cell tests indicates that results should be interpreted with caution in SOT recipients and PWH and that SARS-CoV-2 T-cell tests need to be optimized and further validated for use in vulnerable patient populations. |
| format | Article |
| id | doaj-art-cf332baac2ef4ecabefd3ce669c11759 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-cf332baac2ef4ecabefd3ce669c117592025-02-05T05:32:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031796510.1371/journal.pone.0317965Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function.Annette AudigéAlain AmstutzMacé M SchuurmansPatrizia AmicoDominique L BraunMarcel P StoeckleBarbara HasseRené HageDominik DammMichael TammNicolas J MuellerHuldrych F GünthardMichael T KollerChristof M SchönenbergerAlexandra GriessbachNiklaus D LabhardtRoger D KouyosAlexandra TrkolaMichael HuberKatharina KusejkoHeiner C BucherIrene A AbelaMatthias BrielFrédérique ChammartinBenjamin SpeichSwiss HIV Cohort Study, and the Swiss Transplant Cohort StudyT-cell response plays an important role in SARS-CoV-2 immunogenicity. For people living with HIV (PWH) and solid organ transplant (SOT) recipients there is limited evidence on the reliability of commercially available T-cell tests. We assessed 173 blood samples from 81 participants (62 samples from 35 PWH; 111 samples from 46 SOT recipients [lung and kidney]) with two commercial SARS-CoV-2 Interferon-γ (IFN-γ) release assays (IGRA; SARS-CoV-2 IGRA by Euroimmun, and IGRA SARS-CoV-2 by Roche). The reliability between the tests was judged as low (Cohen's kappa [κ] = 0.20; overall percent agreement [OPA] = 66%). A high proportion of tests were invalid (22% Euroimmun; 8% Roche). When excluding these invalid tests, the agreement was higher (κ = 0.43; OPA = 90%). The low reliability between the two T-cell tests indicates that results should be interpreted with caution in SOT recipients and PWH and that SARS-CoV-2 T-cell tests need to be optimized and further validated for use in vulnerable patient populations.https://doi.org/10.1371/journal.pone.0317965 |
| spellingShingle | Annette Audigé Alain Amstutz Macé M Schuurmans Patrizia Amico Dominique L Braun Marcel P Stoeckle Barbara Hasse René Hage Dominik Damm Michael Tamm Nicolas J Mueller Huldrych F Günthard Michael T Koller Christof M Schönenberger Alexandra Griessbach Niklaus D Labhardt Roger D Kouyos Alexandra Trkola Michael Huber Katharina Kusejko Heiner C Bucher Irene A Abela Matthias Briel Frédérique Chammartin Benjamin Speich Swiss HIV Cohort Study, and the Swiss Transplant Cohort Study Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. PLoS ONE |
| title | Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. |
| title_full | Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. |
| title_fullStr | Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. |
| title_full_unstemmed | Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. |
| title_short | Low agreement and frequent invalid controls in two SARS-CoV-2 T-cell assays in people with compromised immune function. |
| title_sort | low agreement and frequent invalid controls in two sars cov 2 t cell assays in people with compromised immune function |
| url | https://doi.org/10.1371/journal.pone.0317965 |
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