Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells
Introduction. Gastroesophageal reflux has been associated with chronic inflammatory diseases and may be a cause of airway remodelling. Aspiration of gastric fluids may cause damage to airway epithelial cells, not only because acidity is toxic to bronchial epithelial cells, but also since it contains...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.4061/2011/569416 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556126262001664 |
|---|---|
| author | Erik Bathoorn Paul Daly Birgit Gaiser Karl Sternad Craig Poland William MacNee Ellen M. Drost |
| author_facet | Erik Bathoorn Paul Daly Birgit Gaiser Karl Sternad Craig Poland William MacNee Ellen M. Drost |
| author_sort | Erik Bathoorn |
| collection | DOAJ |
| description | Introduction. Gastroesophageal reflux has been associated with chronic inflammatory diseases and may be a cause of airway remodelling. Aspiration of gastric fluids may cause damage to airway epithelial cells, not only because acidity is toxic to bronchial epithelial cells, but also since it contains digestive enzymes, such as pepsin. Aim. To study whether pepsin enhances cytotoxicity and inflammation in airway epithelial cells, and whether this is pH-dependent. Methods. Human bronchial epithelial cells were exposed to increasing pepsin concentrations in varying acidic milieus, and cell proliferation and cytokine release were assessed. Results. Cell survival was decreased by pepsin exposure depending on its concentration (F=17.4) and pH level of the medium (F=6.5) (both P<0.01). Pepsin-induced interleukin-8 release was greater at lower pH (F=5.1; P<0.01). Interleukin-6 induction by pepsin was greater at pH 1.5 compared to pH 2.5 (mean difference 434%; P=0.03). Conclusion. Pepsin is cytotoxic to bronchial epithelial cells and induces inflammation in addition to acid alone, dependent on the level of acidity. Future studies should assess whether chronic aspiration causes airway remodelling in chronic inflammatory lung diseases. |
| format | Article |
| id | doaj-art-cf1a99dbe0d840eebf37477dcf9286da |
| institution | Kabale University |
| issn | 2042-0099 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-cf1a99dbe0d840eebf37477dcf9286da2025-02-03T05:46:19ZengWileyInternational Journal of Inflammation2042-00992011-01-01201110.4061/2011/569416569416Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial CellsErik Bathoorn0Paul Daly1Birgit Gaiser2Karl Sternad3Craig Poland4William MacNee5Ellen M. Drost6ELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKELEGI, Colt Research Laboratories, MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH 16 4TJ, UKIntroduction. Gastroesophageal reflux has been associated with chronic inflammatory diseases and may be a cause of airway remodelling. Aspiration of gastric fluids may cause damage to airway epithelial cells, not only because acidity is toxic to bronchial epithelial cells, but also since it contains digestive enzymes, such as pepsin. Aim. To study whether pepsin enhances cytotoxicity and inflammation in airway epithelial cells, and whether this is pH-dependent. Methods. Human bronchial epithelial cells were exposed to increasing pepsin concentrations in varying acidic milieus, and cell proliferation and cytokine release were assessed. Results. Cell survival was decreased by pepsin exposure depending on its concentration (F=17.4) and pH level of the medium (F=6.5) (both P<0.01). Pepsin-induced interleukin-8 release was greater at lower pH (F=5.1; P<0.01). Interleukin-6 induction by pepsin was greater at pH 1.5 compared to pH 2.5 (mean difference 434%; P=0.03). Conclusion. Pepsin is cytotoxic to bronchial epithelial cells and induces inflammation in addition to acid alone, dependent on the level of acidity. Future studies should assess whether chronic aspiration causes airway remodelling in chronic inflammatory lung diseases.http://dx.doi.org/10.4061/2011/569416 |
| spellingShingle | Erik Bathoorn Paul Daly Birgit Gaiser Karl Sternad Craig Poland William MacNee Ellen M. Drost Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells International Journal of Inflammation |
| title | Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells |
| title_full | Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells |
| title_fullStr | Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells |
| title_full_unstemmed | Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells |
| title_short | Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells |
| title_sort | cytotoxicity and induction of inflammation by pepsin in acid in bronchial epithelial cells |
| url | http://dx.doi.org/10.4061/2011/569416 |
| work_keys_str_mv | AT erikbathoorn cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT pauldaly cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT birgitgaiser cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT karlsternad cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT craigpoland cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT williammacnee cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells AT ellenmdrost cytotoxicityandinductionofinflammationbypepsininacidinbronchialepithelialcells |