Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells
Our previous research demonstrated the hypoglycemic and antioxidant properties of crude polysaccharides from Berberis dasystachya M. (BDP), but the structural characteristics and underlying mechanisms were unclear. In this study, a homogeneous polysaccharide, BDP-I, was isolated and structurally cha...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-08-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1643051/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849397106906759168 |
|---|---|
| author | Xiaohang Lu Lijuan Han Yongrong Deng |
| author_facet | Xiaohang Lu Lijuan Han Yongrong Deng |
| author_sort | Xiaohang Lu |
| collection | DOAJ |
| description | Our previous research demonstrated the hypoglycemic and antioxidant properties of crude polysaccharides from Berberis dasystachya M. (BDP), but the structural characteristics and underlying mechanisms were unclear. In this study, a homogeneous polysaccharide, BDP-I, was isolated and structurally characterized. BDP-I had a molecular weight of 3.947 kDa and consisted of arabinose, galactose, glucose, and galacturonic acid, mainly linked through →6)-β-D-GalAp-(1→ glycosidic bonds.An oxidative stress model was established by treating RIN-m5F pancreatic islet cells with 250 μmol/L H2O2 for 3 hours. BDP-I intervention at concentrations ranging from 0.0625 to 0.5 mg/mL significantly improved cell viability, reduced reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS), and enhanced superoxide dismutase (SOD) and catalase (CAT) activities in a dose-dependent manner.Untargeted metabolomics revealed that BDP-I regulated 46 potential biomarkers, effectively reversing metabolic disturbances caused by oxidative stress. In addition, BDP-I modulated the expression of genes associated with apoptosis and mitochondrial damage, including iNOS and NF-κB.These findings suggest that BDP-I exerts protective effects against oxidative damage in pancreatic islet cells through multiple mechanisms, highlighting its potential as a targeted antioxidant agent for functional food or therapeutic applications. |
| format | Article |
| id | doaj-art-cf156932c92e4032b9fb6e549a9b18c5 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-cf156932c92e4032b9fb6e549a9b18c52025-08-20T03:39:09ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-08-011210.3389/fnut.2025.16430511643051Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cellsXiaohang Lu0Lijuan Han1Yongrong Deng2State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaCollege of Agriculture and Animal Husbandry, Qinghai University, Xining, ChinaOur previous research demonstrated the hypoglycemic and antioxidant properties of crude polysaccharides from Berberis dasystachya M. (BDP), but the structural characteristics and underlying mechanisms were unclear. In this study, a homogeneous polysaccharide, BDP-I, was isolated and structurally characterized. BDP-I had a molecular weight of 3.947 kDa and consisted of arabinose, galactose, glucose, and galacturonic acid, mainly linked through →6)-β-D-GalAp-(1→ glycosidic bonds.An oxidative stress model was established by treating RIN-m5F pancreatic islet cells with 250 μmol/L H2O2 for 3 hours. BDP-I intervention at concentrations ranging from 0.0625 to 0.5 mg/mL significantly improved cell viability, reduced reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS), and enhanced superoxide dismutase (SOD) and catalase (CAT) activities in a dose-dependent manner.Untargeted metabolomics revealed that BDP-I regulated 46 potential biomarkers, effectively reversing metabolic disturbances caused by oxidative stress. In addition, BDP-I modulated the expression of genes associated with apoptosis and mitochondrial damage, including iNOS and NF-κB.These findings suggest that BDP-I exerts protective effects against oxidative damage in pancreatic islet cells through multiple mechanisms, highlighting its potential as a targeted antioxidant agent for functional food or therapeutic applications.https://www.frontiersin.org/articles/10.3389/fnut.2025.1643051/fullBerberis dasystachya M.metabolomicsoxidative stresspancreatic β-cellspolysaccharidesstructural characterization |
| spellingShingle | Xiaohang Lu Lijuan Han Yongrong Deng Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells Frontiers in Nutrition Berberis dasystachya M. metabolomics oxidative stress pancreatic β-cells polysaccharides structural characterization |
| title | Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells |
| title_full | Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells |
| title_fullStr | Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells |
| title_full_unstemmed | Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells |
| title_short | Structural characterization and protective effects of polysaccharides isolated from Berberis dasystachya M. fruits on H2O2-induced oxidative damage in RINm5F pancreatic β-cells |
| title_sort | structural characterization and protective effects of polysaccharides isolated from berberis dasystachya m fruits on h2o2 induced oxidative damage in rinm5f pancreatic β cells |
| topic | Berberis dasystachya M. metabolomics oxidative stress pancreatic β-cells polysaccharides structural characterization |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1643051/full |
| work_keys_str_mv | AT xiaohanglu structuralcharacterizationandprotectiveeffectsofpolysaccharidesisolatedfromberberisdasystachyamfruitsonh2o2inducedoxidativedamageinrinm5fpancreaticbcells AT lijuanhan structuralcharacterizationandprotectiveeffectsofpolysaccharidesisolatedfromberberisdasystachyamfruitsonh2o2inducedoxidativedamageinrinm5fpancreaticbcells AT yongrongdeng structuralcharacterizationandprotectiveeffectsofpolysaccharidesisolatedfromberberisdasystachyamfruitsonh2o2inducedoxidativedamageinrinm5fpancreaticbcells |