Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response
Objectives. Genital herpes simplex virus (HSV) infection is controlled by HSV-specific T cells in the genital tract, and the role of systemic T cell responses is not fully understood. Thus, we analysed T cell responses in patients with recurrent genital herpes (GH). Methods. T cell responses to HSV-...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/5640960 |
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| author | Michal Holub Alžběta Stráníková Pavel Chalupa Simona Arientová Kateřina Roubalová Ondřej Beran |
| author_facet | Michal Holub Alžběta Stráníková Pavel Chalupa Simona Arientová Kateřina Roubalová Ondřej Beran |
| author_sort | Michal Holub |
| collection | DOAJ |
| description | Objectives. Genital herpes simplex virus (HSV) infection is controlled by HSV-specific T cells in the genital tract, and the role of systemic T cell responses is not fully understood. Thus, we analysed T cell responses in patients with recurrent genital herpes (GH). Methods. T cell responses to HSV-1 and HSV-2 native antigens and the expression of HLA-DR and CD38 molecules on circulating CD8+ T cells were analysed in adults with high frequency of GH recurrences (19 patients) and low frequency of GH recurrences (7 patients) and 12 HSV-2 seronegative healthy controls. The study utilized the interferon-γ Elispot assay for measurement of spot-forming cells (SFC) after ex vivo stimulation with HSV antigens and flow cytometry for analysis of the expression of activation markers in unstimulated T cells. Results. The patients with high frequency of GH recurrences (mean number of recurrences of 13.3 per year) had significantly enhanced HSV-specific T cell responses than the HSV-2 seronegative healthy controls. Moreover, a trend of higher numbers of SFC was observed in these patients when compared with those with low frequency of GH recurrences (mean number of recurrences of 3.3 per year). Additionally, no differences in CD38 and HLA-DR expression on circulating CD8+ T cells were found among the study groups. Conclusions. Frequency of GH recurrences positively correlates with high numbers of systemic HSV-specific T cells. |
| format | Article |
| id | doaj-art-cf117c995eaf4c9fa4bf79bfbb232ddd |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-cf117c995eaf4c9fa4bf79bfbb232ddd2025-02-03T01:27:05ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932020-01-01202010.1155/2020/56409605640960Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell ResponseMichal Holub0Alžběta Stráníková1Pavel Chalupa2Simona Arientová3Kateřina Roubalová4Ondřej Beran5Department of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, U Vojenské nemocnice 1200, 169 02 Prague 6, Czech RepublicDepartment of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, U Vojenské nemocnice 1200, 169 02 Prague 6, Czech RepublicDepartment of Infectious and Tropical Diseases, First Faculty of Medicine, Charles University and Na Bulovce Hospital, Budínova 2, 180 81 Prague 8, Czech RepublicDepartment of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, U Vojenské nemocnice 1200, 169 02 Prague 6, Czech RepublicInstitute of Haematology and Blood Transfusion, Department of Immunology, U Nemocnice 2094/1, 128 20 Prague, Czech RepublicDepartment of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, U Vojenské nemocnice 1200, 169 02 Prague 6, Czech RepublicObjectives. Genital herpes simplex virus (HSV) infection is controlled by HSV-specific T cells in the genital tract, and the role of systemic T cell responses is not fully understood. Thus, we analysed T cell responses in patients with recurrent genital herpes (GH). Methods. T cell responses to HSV-1 and HSV-2 native antigens and the expression of HLA-DR and CD38 molecules on circulating CD8+ T cells were analysed in adults with high frequency of GH recurrences (19 patients) and low frequency of GH recurrences (7 patients) and 12 HSV-2 seronegative healthy controls. The study utilized the interferon-γ Elispot assay for measurement of spot-forming cells (SFC) after ex vivo stimulation with HSV antigens and flow cytometry for analysis of the expression of activation markers in unstimulated T cells. Results. The patients with high frequency of GH recurrences (mean number of recurrences of 13.3 per year) had significantly enhanced HSV-specific T cell responses than the HSV-2 seronegative healthy controls. Moreover, a trend of higher numbers of SFC was observed in these patients when compared with those with low frequency of GH recurrences (mean number of recurrences of 3.3 per year). Additionally, no differences in CD38 and HLA-DR expression on circulating CD8+ T cells were found among the study groups. Conclusions. Frequency of GH recurrences positively correlates with high numbers of systemic HSV-specific T cells.http://dx.doi.org/10.1155/2020/5640960 |
| spellingShingle | Michal Holub Alžběta Stráníková Pavel Chalupa Simona Arientová Kateřina Roubalová Ondřej Beran Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response |
| title_full | Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response |
| title_fullStr | Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response |
| title_full_unstemmed | Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response |
| title_short | Frequent Recurrences of Genital Herpes Are Associated with Enhanced Systemic HSV-Specific T Cell Response |
| title_sort | frequent recurrences of genital herpes are associated with enhanced systemic hsv specific t cell response |
| url | http://dx.doi.org/10.1155/2020/5640960 |
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