Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation
Epidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question addressed here is to determine whether the culture process may induce cellular, molecular, or genetic alterations that might increase the risk of cellular transformati...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Skin Cancer |
| Online Access: | http://dx.doi.org/10.1155/2011/936546 |
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| _version_ | 1832553637438554112 |
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| author | A. Thépot A. Desanlis E. Venet L. Thivillier V. Justin A. P. Morel F. DeFraipont M. Till V. Krutovskikh M. Tommasino O. Damour P. Hainaut |
| author_facet | A. Thépot A. Desanlis E. Venet L. Thivillier V. Justin A. P. Morel F. DeFraipont M. Till V. Krutovskikh M. Tommasino O. Damour P. Hainaut |
| author_sort | A. Thépot |
| collection | DOAJ |
| description | Epidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question addressed here is to determine whether the culture process may induce cellular, molecular, or genetic alterations that might increase the risk of cellular transformation. Keratinocytes from four different human donors were investigated for molecular and cellular parameters indicative of transformation status, including (i) karyotype, (ii) telomere length, (iii) proliferation rate, (iv) epithelial-mesenchymal transition, (v) anchorage-independent growth potential, and (vi) tumorigenicity in nude mice. Results show that, despite increased cell survival in one keratinocyte strain, none of the cultures displayed characteristics of cell transformations, implying that the culture protocol does not generate artefacts leading to the selection of transformed cells. We conclude that the current protocol does not result in an increased risk of tumorigenicity of transplanted cells. |
| format | Article |
| id | doaj-art-cf0b63a3a60f4a1aa255b87811a60c67 |
| institution | Kabale University |
| issn | 2090-2905 2090-2913 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Skin Cancer |
| spelling | doaj-art-cf0b63a3a60f4a1aa255b87811a60c672025-02-03T05:53:37ZengWileyJournal of Skin Cancer2090-29052090-29132011-01-01201110.1155/2011/936546936546Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet TransplantationA. Thépot0A. Desanlis1E. Venet2L. Thivillier3V. Justin4A. P. Morel5F. DeFraipont6M. Till7V. Krutovskikh8M. Tommasino9O. Damour10P. Hainaut11Banque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceBanque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceBanque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceBanque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceBanque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceCentre Léon Bérard, 69373 Lyon cedex 03, FranceCentre Hospitalier Universitaire, 38043 Grenoble cedex 09, FranceLaboratoire de Cytogénétique HFME, 69677 Bron, FranceCentre International de Recherche sur le Cancer, 69372 Lyon cedex 08, FranceCentre International de Recherche sur le Cancer, 69372 Lyon cedex 08, FranceBanque de Tissus et Cellules, Hôpital E. Herriot, 69437 Lyon cedex 03, FranceCentre International de Recherche sur le Cancer, 69372 Lyon cedex 08, FranceEpidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question addressed here is to determine whether the culture process may induce cellular, molecular, or genetic alterations that might increase the risk of cellular transformation. Keratinocytes from four different human donors were investigated for molecular and cellular parameters indicative of transformation status, including (i) karyotype, (ii) telomere length, (iii) proliferation rate, (iv) epithelial-mesenchymal transition, (v) anchorage-independent growth potential, and (vi) tumorigenicity in nude mice. Results show that, despite increased cell survival in one keratinocyte strain, none of the cultures displayed characteristics of cell transformations, implying that the culture protocol does not generate artefacts leading to the selection of transformed cells. We conclude that the current protocol does not result in an increased risk of tumorigenicity of transplanted cells.http://dx.doi.org/10.1155/2011/936546 |
| spellingShingle | A. Thépot A. Desanlis E. Venet L. Thivillier V. Justin A. P. Morel F. DeFraipont M. Till V. Krutovskikh M. Tommasino O. Damour P. Hainaut Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation Journal of Skin Cancer |
| title | Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation |
| title_full | Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation |
| title_fullStr | Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation |
| title_full_unstemmed | Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation |
| title_short | Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation |
| title_sort | assessment of transformed properties in vitro and of tumorigenicity in vivo in primary keratinocytes cultured for epidermal sheet transplantation |
| url | http://dx.doi.org/10.1155/2011/936546 |
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