Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway
Objective. To study the effect of Codonopsis pilosula polysaccharide (CPP) on the growth and motility of HepG2 cells and its possible mechanism. Methods. Cells were randomly divided into Control group, CPP (5 μM) group, CPP (10 μM) group, and CPP (20 μM) group. The proliferation, invasion, migration...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2019/7068437 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554180425809920 |
|---|---|
| author | Yuan-yuan Zhang Ying-mei Zhang Hai-yan Xu |
| author_facet | Yuan-yuan Zhang Ying-mei Zhang Hai-yan Xu |
| author_sort | Yuan-yuan Zhang |
| collection | DOAJ |
| description | Objective. To study the effect of Codonopsis pilosula polysaccharide (CPP) on the growth and motility of HepG2 cells and its possible mechanism. Methods. Cells were randomly divided into Control group, CPP (5 μM) group, CPP (10 μM) group, and CPP (20 μM) group. The proliferation, invasion, migration ability, and expression of proteins involved in the epithelial-mesenchymal transition (EMT) and signaling pathway of HepG2 cells were detected by CCK8 assay, BrdU staining, Transwell, Scratch test, and Western blot, respectively. Results. Codonopsis pilosula polysaccharide inhibited the proliferation of HepG2 cells cultured in vitro along with the expression level of Ki67 and PCNA protein (P<0.05), decreased the number of invasive cells (P<0.05), and reduced the scratch closure rate (P<0.05). It also adjusted the expression of vascular endothelial growth factor (VEGF), E-cadherin, and N-cadherin (P<0.05). Other than that, downregulation of β-catenin, TCF4, and c-Myc protein expression (P<0.05) was observed as well. Conclusion. Codonopsis pilosula polysaccharide can inhibit the proliferation and motility of HepG2 cells cultured in vitro, and the underlying mechanism is proposed to be related to the inhibition of the β-catenin/TCF4 pathway. |
| format | Article |
| id | doaj-art-cefe1007110b4604832293f38864c5f3 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-cefe1007110b4604832293f38864c5f32025-02-03T05:52:12ZengWileyInternational Journal of Polymer Science1687-94221687-94302019-01-01201910.1155/2019/70684377068437Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 PathwayYuan-yuan Zhang0Ying-mei Zhang1Hai-yan Xu2Department of Laboratory Medicine, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, ChinaDepartment of Laboratory Medicine, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, ChinaDepartment of Cardiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, ChinaObjective. To study the effect of Codonopsis pilosula polysaccharide (CPP) on the growth and motility of HepG2 cells and its possible mechanism. Methods. Cells were randomly divided into Control group, CPP (5 μM) group, CPP (10 μM) group, and CPP (20 μM) group. The proliferation, invasion, migration ability, and expression of proteins involved in the epithelial-mesenchymal transition (EMT) and signaling pathway of HepG2 cells were detected by CCK8 assay, BrdU staining, Transwell, Scratch test, and Western blot, respectively. Results. Codonopsis pilosula polysaccharide inhibited the proliferation of HepG2 cells cultured in vitro along with the expression level of Ki67 and PCNA protein (P<0.05), decreased the number of invasive cells (P<0.05), and reduced the scratch closure rate (P<0.05). It also adjusted the expression of vascular endothelial growth factor (VEGF), E-cadherin, and N-cadherin (P<0.05). Other than that, downregulation of β-catenin, TCF4, and c-Myc protein expression (P<0.05) was observed as well. Conclusion. Codonopsis pilosula polysaccharide can inhibit the proliferation and motility of HepG2 cells cultured in vitro, and the underlying mechanism is proposed to be related to the inhibition of the β-catenin/TCF4 pathway.http://dx.doi.org/10.1155/2019/7068437 |
| spellingShingle | Yuan-yuan Zhang Ying-mei Zhang Hai-yan Xu Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway International Journal of Polymer Science |
| title | Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway |
| title_full | Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway |
| title_fullStr | Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway |
| title_full_unstemmed | Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway |
| title_short | Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway |
| title_sort | effect of codonopsis pilosula polysaccharides on the growth and motility of hepatocellular carcinoma hepg2 cells by regulating β catenin tcf4 pathway |
| url | http://dx.doi.org/10.1155/2019/7068437 |
| work_keys_str_mv | AT yuanyuanzhang effectofcodonopsispilosulapolysaccharidesonthegrowthandmotilityofhepatocellularcarcinomahepg2cellsbyregulatingbcatenintcf4pathway AT yingmeizhang effectofcodonopsispilosulapolysaccharidesonthegrowthandmotilityofhepatocellularcarcinomahepg2cellsbyregulatingbcatenintcf4pathway AT haiyanxu effectofcodonopsispilosulapolysaccharidesonthegrowthandmotilityofhepatocellularcarcinomahepg2cellsbyregulatingbcatenintcf4pathway |