Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus.
The budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. In the cytoplasm, apart from catalyzing 5'→3' decay onto non translated mRNAs, Xrn1 can follow the last translating ribosom...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0308195 |
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| author | Antonio Jordán-Pla Yujie Zhang José García-Martínez Shiladitya Chattopadhyay Anabel Forte Mordechai Choder Vicent Pelechano José E Pérez-Ortín |
| author_facet | Antonio Jordán-Pla Yujie Zhang José García-Martínez Shiladitya Chattopadhyay Anabel Forte Mordechai Choder Vicent Pelechano José E Pérez-Ortín |
| author_sort | Antonio Jordán-Pla |
| collection | DOAJ |
| description | The budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. In the cytoplasm, apart from catalyzing 5'→3' decay onto non translated mRNAs, Xrn1 can follow the last translating ribosome to degrade the decapped mRNA template, a process known as "cotranslational mRNA decay". We have previously observed that the import of Xrn1 to the nucleus is required for efficient cytoplasmic mRNA decay. Here by using an Xrn1 mutant that cannot enter the nucleus, but is otherwise functional in ribonuclease activity, we show that nuclear import is necessary for proper global cotranslational decay of mRNAs along coding regions and also affects degradation in the of 5' region of a large group of mRNAs, which comprise about 20% of the transcriptome. Furthermore, a principal component analysis of the genomic datasets of this mutant and other Xrn1 mutants also shows that lack of a cytoplasmic 5'→3' exoribonuclease is the primary cause of the physiological defects seen in a xrn1Δ mutant, but also suggests that Xrn1 import into the nucleus is necessary for its full in vivo functions. |
| format | Article |
| id | doaj-art-cee0a1d0c8114291b0374adee5623907 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-cee0a1d0c8114291b0374adee56239072025-02-05T05:31:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e030819510.1371/journal.pone.0308195Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus.Antonio Jordán-PlaYujie ZhangJosé García-MartínezShiladitya ChattopadhyayAnabel ForteMordechai ChoderVicent PelechanoJosé E Pérez-OrtínThe budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. In the cytoplasm, apart from catalyzing 5'→3' decay onto non translated mRNAs, Xrn1 can follow the last translating ribosome to degrade the decapped mRNA template, a process known as "cotranslational mRNA decay". We have previously observed that the import of Xrn1 to the nucleus is required for efficient cytoplasmic mRNA decay. Here by using an Xrn1 mutant that cannot enter the nucleus, but is otherwise functional in ribonuclease activity, we show that nuclear import is necessary for proper global cotranslational decay of mRNAs along coding regions and also affects degradation in the of 5' region of a large group of mRNAs, which comprise about 20% of the transcriptome. Furthermore, a principal component analysis of the genomic datasets of this mutant and other Xrn1 mutants also shows that lack of a cytoplasmic 5'→3' exoribonuclease is the primary cause of the physiological defects seen in a xrn1Δ mutant, but also suggests that Xrn1 import into the nucleus is necessary for its full in vivo functions.https://doi.org/10.1371/journal.pone.0308195 |
| spellingShingle | Antonio Jordán-Pla Yujie Zhang José García-Martínez Shiladitya Chattopadhyay Anabel Forte Mordechai Choder Vicent Pelechano José E Pérez-Ortín Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. PLoS ONE |
| title | Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. |
| title_full | Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. |
| title_fullStr | Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. |
| title_full_unstemmed | Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. |
| title_short | Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. |
| title_sort | proper 5 3 cotranslational mrna decay in yeast requires import of xrn1 to the nucleus |
| url | https://doi.org/10.1371/journal.pone.0308195 |
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