FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds
Recent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. Diacylglycerol acyltransferase 2 (DGAT2), which catalyzes the final step in triacylglycerol (TAG) assembly, might potentially regulate the biosynthesis of...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/390672 |
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| author | Yi-Cun Chen Yang-Dong Wang Qin-Qin Cui Zhi-Yong Zhan |
| author_facet | Yi-Cun Chen Yang-Dong Wang Qin-Qin Cui Zhi-Yong Zhan |
| author_sort | Yi-Cun Chen |
| collection | DOAJ |
| description | Recent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. Diacylglycerol acyltransferase 2 (DGAT2), which catalyzes the final step in triacylglycerol (TAG) assembly, might potentially regulate the biosynthesis of desired fatty acids in TAGs. To study the effects of tung tree (Vernicia fordii) vfDGAT2 in channeling the desired fatty acids into TAG, vfDGAT2 combined with the tung tree fatty acid desaturase-2 (vfFAD2) gene was co-introduced into Aspergillus fumigatus, an endophytic fungus isolated from healthy tung oilseed. Two transformants coexpressing vfFAD2 and vfDGAT2 showed a more than 6-fold increase in linoleic acid production compared to the original A. fumigatus strain, while a nearly 2-fold increase was found in the transformant expressing only vfFAD2. Our data suggest that vfDGAT2 plays a pivotal role in promoting linoleic acid accumulation in TAGs. This holds great promise for further genetic engineering aimed at producing valuable fatty acids. |
| format | Article |
| id | doaj-art-ce76bd7a214a4cc29265ee16a620a2e8 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ce76bd7a214a4cc29265ee16a620a2e82025-02-03T07:25:40ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/390672390672FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung OilseedsYi-Cun Chen0Yang-Dong Wang1Qin-Qin Cui2Zhi-Yong Zhan3State key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaState key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaState key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaState key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, Beijing 100091, ChinaRecent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. Diacylglycerol acyltransferase 2 (DGAT2), which catalyzes the final step in triacylglycerol (TAG) assembly, might potentially regulate the biosynthesis of desired fatty acids in TAGs. To study the effects of tung tree (Vernicia fordii) vfDGAT2 in channeling the desired fatty acids into TAG, vfDGAT2 combined with the tung tree fatty acid desaturase-2 (vfFAD2) gene was co-introduced into Aspergillus fumigatus, an endophytic fungus isolated from healthy tung oilseed. Two transformants coexpressing vfFAD2 and vfDGAT2 showed a more than 6-fold increase in linoleic acid production compared to the original A. fumigatus strain, while a nearly 2-fold increase was found in the transformant expressing only vfFAD2. Our data suggest that vfDGAT2 plays a pivotal role in promoting linoleic acid accumulation in TAGs. This holds great promise for further genetic engineering aimed at producing valuable fatty acids.http://dx.doi.org/10.1100/2012/390672 |
| spellingShingle | Yi-Cun Chen Yang-Dong Wang Qin-Qin Cui Zhi-Yong Zhan FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds The Scientific World Journal |
| title | FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds |
| title_full | FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds |
| title_fullStr | FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds |
| title_full_unstemmed | FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds |
| title_short | FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds |
| title_sort | fad2 dgat2 genes coexpressed in endophytic aspergillus fumigatus derived from tung oilseeds |
| url | http://dx.doi.org/10.1100/2012/390672 |
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