Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania
Antibiotic resistance is on the WHO’s top 10 list of global public health threats due to its rapid emergence and spread but also because of the high morbidity and mortality associated with it. Amongst the main species driving this phenomenon is <i>A. baumannii</i>, a member of the ESKAPE...
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2025-01-01
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| author | Adrian-Gabriel Pană Pavel Șchiopu Dan Alexandru Țoc Vlad Sever Neculicioiu Anca Butiuc-Keul Anca Farkas Matei-Ștefan Dobrescu Mirela Flonta Carmen Costache Izabella Éva Szász Lia-Monica Junie |
| author_facet | Adrian-Gabriel Pană Pavel Șchiopu Dan Alexandru Țoc Vlad Sever Neculicioiu Anca Butiuc-Keul Anca Farkas Matei-Ștefan Dobrescu Mirela Flonta Carmen Costache Izabella Éva Szász Lia-Monica Junie |
| author_sort | Adrian-Gabriel Pană |
| collection | DOAJ |
| description | Antibiotic resistance is on the WHO’s top 10 list of global public health threats due to its rapid emergence and spread but also because of the high morbidity and mortality associated with it. Amongst the main species driving this phenomenon is <i>A. baumannii</i>, a member of the ESKAPE group of medical assistance-associated infections causing species famous for its extensively drug-resistant phenotypes. Our findings note a 91.52% frequency of extensively drug-resistant carbapenem-resistant <i>A. baumannii</i> (XDR CRAB) phenotype amongst clinical isolates from multiple hospitals in two major cities from northwestern and central Romania, harboring multiple antibiotic resistance genes such as bla<i><sub>OXA-23-like</sub></i> in 108 (91.5%) isolates, bla<i><sub>OXA-24/40-like</sub></i> in 88 (74.6%) isolates, bla<i><sub>NDM</sub></i> in 29 (25%) isolates, <i>ArmA</i> in 75 (63.6%) isolates, and <i>ant(3″)-I</i> in 69 (58.5%) isolates and <i>sul1</i> in 113 (95.76%) isolates. The isolates, although nearly identical in phenotype, displayed different genotypical profiles, with varying degrees of similarity across hospitals and cities, raising the possibility of both local outbreaks of a single clone and widespread dissemination of resistant isolates. |
| format | Article |
| id | doaj-art-ce67044169ea48418cf16d1a3f18fed2 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-ce67044169ea48418cf16d1a3f18fed22025-01-24T13:42:56ZengMDPI AGMicroorganisms2076-26072025-01-0113117610.3390/microorganisms13010176Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from RomaniaAdrian-Gabriel Pană0Pavel Șchiopu1Dan Alexandru Țoc2Vlad Sever Neculicioiu3Anca Butiuc-Keul4Anca Farkas5Matei-Ștefan Dobrescu6Mirela Flonta7Carmen Costache8Izabella Éva Szász9Lia-Monica Junie10Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaDoctoral School of Integrative Biology, Babeș-Bolyai University, 1 M. Kogalniceanu Street, 400084 Cluj-Napoca, RomaniaDoctoral School of Integrative Biology, Babeș-Bolyai University, 1 M. Kogalniceanu Street, 400084 Cluj-Napoca, RomaniaDoctoral School of Integrative Biology, Babeș-Bolyai University, 1 M. Kogalniceanu Street, 400084 Cluj-Napoca, RomaniaInfectious Disease Clinical Hospital, 23 Iuliu Moldovan Street, 400003 Cluj-Napoca, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaTârgu-Mureș Emergency Clinical County Hospital, 50 Gheorghe Marinescu Street, 540136 Târgu-Mureș, RomaniaDepartment of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 4–6 Louis Pasteur Street, 400012 Cluj-Napoca, RomaniaAntibiotic resistance is on the WHO’s top 10 list of global public health threats due to its rapid emergence and spread but also because of the high morbidity and mortality associated with it. Amongst the main species driving this phenomenon is <i>A. baumannii</i>, a member of the ESKAPE group of medical assistance-associated infections causing species famous for its extensively drug-resistant phenotypes. Our findings note a 91.52% frequency of extensively drug-resistant carbapenem-resistant <i>A. baumannii</i> (XDR CRAB) phenotype amongst clinical isolates from multiple hospitals in two major cities from northwestern and central Romania, harboring multiple antibiotic resistance genes such as bla<i><sub>OXA-23-like</sub></i> in 108 (91.5%) isolates, bla<i><sub>OXA-24/40-like</sub></i> in 88 (74.6%) isolates, bla<i><sub>NDM</sub></i> in 29 (25%) isolates, <i>ArmA</i> in 75 (63.6%) isolates, and <i>ant(3″)-I</i> in 69 (58.5%) isolates and <i>sul1</i> in 113 (95.76%) isolates. The isolates, although nearly identical in phenotype, displayed different genotypical profiles, with varying degrees of similarity across hospitals and cities, raising the possibility of both local outbreaks of a single clone and widespread dissemination of resistant isolates.https://www.mdpi.com/2076-2607/13/1/176antibiotic resistance genescarbapenem-resistant <i>Acinetobacter baumannii</i>clonalityESKAPEEuropeextensively drug resistant |
| spellingShingle | Adrian-Gabriel Pană Pavel Șchiopu Dan Alexandru Țoc Vlad Sever Neculicioiu Anca Butiuc-Keul Anca Farkas Matei-Ștefan Dobrescu Mirela Flonta Carmen Costache Izabella Éva Szász Lia-Monica Junie Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania Microorganisms antibiotic resistance genes carbapenem-resistant <i>Acinetobacter baumannii</i> clonality ESKAPE Europe extensively drug resistant |
| title | Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania |
| title_full | Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania |
| title_fullStr | Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania |
| title_full_unstemmed | Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania |
| title_short | Clonality and the Phenotype–Genotype Correlation of Antimicrobial Resistance in <i>Acinetobacter baumannii</i> Isolates: A Multicenter Study of Clinical Isolates from Romania |
| title_sort | clonality and the phenotype genotype correlation of antimicrobial resistance in i acinetobacter baumannii i isolates a multicenter study of clinical isolates from romania |
| topic | antibiotic resistance genes carbapenem-resistant <i>Acinetobacter baumannii</i> clonality ESKAPE Europe extensively drug resistant |
| url | https://www.mdpi.com/2076-2607/13/1/176 |
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