Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome
Background/Objective: Metabolic syndrome (MetS) is characterized by abdominal obesity, increased blood pressure (BP), fasting blood glucose (FBG) and triglyceride levels, and reduced high-density lipoprotein (HDL) levels. This study aims to investigate the efficacy of the Wharton’s jelly mesenchymal...
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2025-01-01
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| author | Illayaraja Krishnan Magdalene Tan Mei Ling Min Hwei Ng Jia Xian Law Mohd Rafizul Mohd Yusof Thavachelvi Thangarajah Zalina Mahmood Nurul Izzati Uda Zahli Shathiya Rajamanickam Baskar Subramani Yogeswaran Lokanathan |
| author_facet | Illayaraja Krishnan Magdalene Tan Mei Ling Min Hwei Ng Jia Xian Law Mohd Rafizul Mohd Yusof Thavachelvi Thangarajah Zalina Mahmood Nurul Izzati Uda Zahli Shathiya Rajamanickam Baskar Subramani Yogeswaran Lokanathan |
| author_sort | Illayaraja Krishnan |
| collection | DOAJ |
| description | Background/Objective: Metabolic syndrome (MetS) is characterized by abdominal obesity, increased blood pressure (BP), fasting blood glucose (FBG) and triglyceride levels, and reduced high-density lipoprotein (HDL) levels. This study aims to investigate the efficacy of the Wharton’s jelly mesenchymal stem cells (WJMSCs)-derived small extracellular vesicles’ (sEVs) preparations in managing MetS. Method: Twenty-four rats were fed with a high-fat and high-fructose diet to induce MetS for 16 weeks and randomized into three groups (<i>n</i> = 8/group): a MetS Control group treated with normal saline, MetS Low Dose (LD) group treated with a LD of sEVs preparations (3 × 10<sup>9</sup> particle/rat), and MetS High Dose (HD) group treated with a HD of sEVs preparations (9 × 10<sup>9</sup> particles/rat). The Control Non-Disease (ND) group was given a standard rat diet and autoclaved tap water with normal saline as treatment. Treatments were given via intravenous injection every three weeks for twelve weeks. Rats were assessed every six weeks for physical measurements, FBG, lipid profiles, CRP, leptin, adiponectin, and BP. Necropsy evaluation was performed on the lungs, liver, spleen, and kidney. Results: Significant reductions in FBG, triglycerides, BP, and increased HDL levels were observed in the treated groups compared to the control group. However, significant abdominal circumference (AC) improvement was not observed in the treated groups. Non-significant associations were found between fasting CRP, leptin, and adiponectin levels with MetS rats after treatment. In addition, sEVs preparations improved inflammation and hemorrhage in the lung and mineralisation in the renal of the treated group. Conclusions: Human fetal WJMSCs-derived sEVs preparations improve all the clusters of MetS in rats except AC and could be further explored as a treatment for MetS. |
| format | Article |
| id | doaj-art-ce563c61b2454ac3ae964a5a4511bb71 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-ce563c61b2454ac3ae964a5a4511bb712025-01-24T13:24:58ZengMDPI AGBiomolecules2218-273X2025-01-011514410.3390/biom15010044Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic SyndromeIllayaraja Krishnan0Magdalene Tan Mei Ling1Min Hwei Ng2Jia Xian Law3Mohd Rafizul Mohd Yusof4Thavachelvi Thangarajah5Zalina Mahmood6Nurul Izzati Uda Zahli7Shathiya Rajamanickam8Baskar Subramani9Yogeswaran Lokanathan10Department of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaDepartment of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaDepartment of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaDepartment of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaDepartment of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaDepartment of Obstetrics and Gynaecology, Hospital Angkatan Tentera (HAT) Tuanku Mizan, Wangsa Maju, Kuala Lumpur 53300, MalaysiaProduction and Blood Supply Management Division, National Blood Centre, Jalan Tun Razak, Kuala Lumpur 50400, MalaysiaDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), Serdang 43400, MalaysiaMedixcell Sdn. Bhd., Level 5, Equatorial Plaza, Lot 5-5 & 5-6, Jalan Sultan Ismail, Kuala Lumpur 50250, MalaysiaMedixcell Sdn. Bhd., Level 5, Equatorial Plaza, Lot 5-5 & 5-6, Jalan Sultan Ismail, Kuala Lumpur 50250, MalaysiaDepartment of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur 56000, MalaysiaBackground/Objective: Metabolic syndrome (MetS) is characterized by abdominal obesity, increased blood pressure (BP), fasting blood glucose (FBG) and triglyceride levels, and reduced high-density lipoprotein (HDL) levels. This study aims to investigate the efficacy of the Wharton’s jelly mesenchymal stem cells (WJMSCs)-derived small extracellular vesicles’ (sEVs) preparations in managing MetS. Method: Twenty-four rats were fed with a high-fat and high-fructose diet to induce MetS for 16 weeks and randomized into three groups (<i>n</i> = 8/group): a MetS Control group treated with normal saline, MetS Low Dose (LD) group treated with a LD of sEVs preparations (3 × 10<sup>9</sup> particle/rat), and MetS High Dose (HD) group treated with a HD of sEVs preparations (9 × 10<sup>9</sup> particles/rat). The Control Non-Disease (ND) group was given a standard rat diet and autoclaved tap water with normal saline as treatment. Treatments were given via intravenous injection every three weeks for twelve weeks. Rats were assessed every six weeks for physical measurements, FBG, lipid profiles, CRP, leptin, adiponectin, and BP. Necropsy evaluation was performed on the lungs, liver, spleen, and kidney. Results: Significant reductions in FBG, triglycerides, BP, and increased HDL levels were observed in the treated groups compared to the control group. However, significant abdominal circumference (AC) improvement was not observed in the treated groups. Non-significant associations were found between fasting CRP, leptin, and adiponectin levels with MetS rats after treatment. In addition, sEVs preparations improved inflammation and hemorrhage in the lung and mineralisation in the renal of the treated group. Conclusions: Human fetal WJMSCs-derived sEVs preparations improve all the clusters of MetS in rats except AC and could be further explored as a treatment for MetS.https://www.mdpi.com/2218-273X/15/1/44umbilical cordexosomesmetabolic diseasessyndrome Xbiotherapeutics |
| spellingShingle | Illayaraja Krishnan Magdalene Tan Mei Ling Min Hwei Ng Jia Xian Law Mohd Rafizul Mohd Yusof Thavachelvi Thangarajah Zalina Mahmood Nurul Izzati Uda Zahli Shathiya Rajamanickam Baskar Subramani Yogeswaran Lokanathan Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome Biomolecules umbilical cord exosomes metabolic diseases syndrome X biotherapeutics |
| title | Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome |
| title_full | Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome |
| title_fullStr | Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome |
| title_full_unstemmed | Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome |
| title_short | Efficacy of Fetal Wharton’s Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome |
| title_sort | efficacy of fetal wharton s jelly mesenchymal stem cells derived small extracellular vesicles in metabolic syndrome |
| topic | umbilical cord exosomes metabolic diseases syndrome X biotherapeutics |
| url | https://www.mdpi.com/2218-273X/15/1/44 |
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