Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice
Melanoma is one of the most aggressive forms of cancer, usually resistant to standard chemotherapeutics. Despite a huge number of clinical trials, any success to find a chemotherapeutic agent that can effectively destroy melanoma is yet to be achieved. Para-phenylenediamine (p-PD) in the hair dyes i...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/3137010 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559033205129216 |
|---|---|
| author | Debajit Bhowmick Kaushik Bhar Sanjaya K. Mallick Subhadip Das Nabanita Chatterjee Tuhin Subhra Sarkar Rajarshi Chakrabarti Krishna Das Saha Anirban Siddhanta |
| author_facet | Debajit Bhowmick Kaushik Bhar Sanjaya K. Mallick Subhadip Das Nabanita Chatterjee Tuhin Subhra Sarkar Rajarshi Chakrabarti Krishna Das Saha Anirban Siddhanta |
| author_sort | Debajit Bhowmick |
| collection | DOAJ |
| description | Melanoma is one of the most aggressive forms of cancer, usually resistant to standard chemotherapeutics. Despite a huge number of clinical trials, any success to find a chemotherapeutic agent that can effectively destroy melanoma is yet to be achieved. Para-phenylenediamine (p-PD) in the hair dyes is reported to purely serve as an external dyeing agent. Very little is known about whether p-PD has any effect on the melanin producing cells. We have demonstrated p-PD mediated apoptotic death of both human and mouse melanoma cells in vitro. Mouse melanoma tumour growth was also arrested by the apoptotic activity of intraperitoneal administration of p-PD with almost no side effects. This apoptosis is shown to occur primarily via loss of mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS), and caspase 8 activation. p-PD mediated apoptosis was also confirmed by the increase in sub-G0/G1 cell number. Thus, our experimental observation suggests that p-PD can be a potential less expensive candidate to be developed as a chemotherapeutic agent for melanoma. |
| format | Article |
| id | doaj-art-ce518eba67854a8f873adbc5a08b761e |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-ce518eba67854a8f873adbc5a08b761e2025-02-03T01:31:01ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/31370103137010Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in MiceDebajit Bhowmick0Kaushik Bhar1Sanjaya K. Mallick2Subhadip Das3Nabanita Chatterjee4Tuhin Subhra Sarkar5Rajarshi Chakrabarti6Krishna Das Saha7Anirban Siddhanta8Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, IndiaDepartment of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, IndiaCytometry Solutions Pvt Ltd., Tripti Mitra’s Garden, Balia Garia Station Road, Kolkata, West Bengal 700084, IndiaCancer & Cell Biology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, West Bengal 700032, IndiaCancer & Cell Biology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, West Bengal 700032, IndiaDepartment of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, IndiaDepartment of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, IndiaCancer & Cell Biology Division, CSIR-Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata, West Bengal 700032, IndiaDepartment of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, IndiaMelanoma is one of the most aggressive forms of cancer, usually resistant to standard chemotherapeutics. Despite a huge number of clinical trials, any success to find a chemotherapeutic agent that can effectively destroy melanoma is yet to be achieved. Para-phenylenediamine (p-PD) in the hair dyes is reported to purely serve as an external dyeing agent. Very little is known about whether p-PD has any effect on the melanin producing cells. We have demonstrated p-PD mediated apoptotic death of both human and mouse melanoma cells in vitro. Mouse melanoma tumour growth was also arrested by the apoptotic activity of intraperitoneal administration of p-PD with almost no side effects. This apoptosis is shown to occur primarily via loss of mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS), and caspase 8 activation. p-PD mediated apoptosis was also confirmed by the increase in sub-G0/G1 cell number. Thus, our experimental observation suggests that p-PD can be a potential less expensive candidate to be developed as a chemotherapeutic agent for melanoma.http://dx.doi.org/10.1155/2016/3137010 |
| spellingShingle | Debajit Bhowmick Kaushik Bhar Sanjaya K. Mallick Subhadip Das Nabanita Chatterjee Tuhin Subhra Sarkar Rajarshi Chakrabarti Krishna Das Saha Anirban Siddhanta Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice Biochemistry Research International |
| title | Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice |
| title_full | Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice |
| title_fullStr | Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice |
| title_full_unstemmed | Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice |
| title_short | Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice |
| title_sort | para phenylenediamine induces apoptotic death of melanoma cells and reduces melanoma tumour growth in mice |
| url | http://dx.doi.org/10.1155/2016/3137010 |
| work_keys_str_mv | AT debajitbhowmick paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT kaushikbhar paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT sanjayakmallick paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT subhadipdas paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT nabanitachatterjee paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT tuhinsubhrasarkar paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT rajarshichakrabarti paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT krishnadassaha paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice AT anirbansiddhanta paraphenylenediamineinducesapoptoticdeathofmelanomacellsandreducesmelanomatumourgrowthinmice |