Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles
Field application of collagen obtained from marine organisms is in wide practice. Moreover, its use could be conditioned depending on the desired purpose and structural characteristics. Therefore, further studies on collagen crosslinking are needed to determine how this may affect the properties of...
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Elsevier
2025-06-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225001428 |
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| author | Angel Edgardo Tapia-Vasquez Wilfrido Torres-Arreola Josafat Marina Ezquerra-Brauer Enrique Márquez-Ríos Hisila Santacruz-Ortega Juan Carlos Ramírez-Suárez Guillermina García-Sánchez Guadalupe Miroslava Suárez-Jiménez |
| author_facet | Angel Edgardo Tapia-Vasquez Wilfrido Torres-Arreola Josafat Marina Ezquerra-Brauer Enrique Márquez-Ríos Hisila Santacruz-Ortega Juan Carlos Ramírez-Suárez Guillermina García-Sánchez Guadalupe Miroslava Suárez-Jiménez |
| author_sort | Angel Edgardo Tapia-Vasquez |
| collection | DOAJ |
| description | Field application of collagen obtained from marine organisms is in wide practice. Moreover, its use could be conditioned depending on the desired purpose and structural characteristics. Therefore, further studies on collagen crosslinking are needed to determine how this may affect the properties of collagen. A spectrophotometric characterization of the collagen found in Octopus vulgaris and Dosidicus gigas arms was carried out. The 1H NMR and fluorometric spectra confirmed the presence of pyridinoline as the main molecule responsible for collagen crosslinking. Using the amino acid profile and 1H NMR spectra, it was determined that the jumbo squid has a relatively high pyridinoline content. The viscosity properties of this collagen were found to exhibit the behaviour of a pseudoplastic fluid. Finally, under the conditions proposed in this study, the degree of crosslinking of the collagen of the octopus and the jumbo squid did not affect the viscosity properties of the protein. |
| format | Article |
| id | doaj-art-ce2fb5ec309b4a57abfe587b9f3dc9d2 |
| institution | DOAJ |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-ce2fb5ec309b4a57abfe587b9f3dc9d22025-08-20T02:39:58ZengElsevierApplied Food Research2772-50222025-06-015110083210.1016/j.afres.2025.100832Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm musclesAngel Edgardo Tapia-Vasquez0Wilfrido Torres-Arreola1Josafat Marina Ezquerra-Brauer2Enrique Márquez-Ríos3Hisila Santacruz-Ortega4Juan Carlos Ramírez-Suárez5Guillermina García-Sánchez6Guadalupe Miroslava Suárez-Jiménez7CONAHCYT - Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora. MéxicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora. México; Corresponding author.Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora. MéxicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora. MéxicoDepartamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n, 83000, Col. Centro. Hermosillo, Sonora, MéxicoCentro de Investigación en Alimentación y Desarrollo A.C., carretera Gustavo Enrique Astiazarán No. 46, Colonia La Victoria, Hermosillo 83304, Sonora, MéxicoCentro de Investigación en Alimentación y Desarrollo A.C., carretera Gustavo Enrique Astiazarán No. 46, Colonia La Victoria, Hermosillo 83304, Sonora, MéxicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora. MéxicoField application of collagen obtained from marine organisms is in wide practice. Moreover, its use could be conditioned depending on the desired purpose and structural characteristics. Therefore, further studies on collagen crosslinking are needed to determine how this may affect the properties of collagen. A spectrophotometric characterization of the collagen found in Octopus vulgaris and Dosidicus gigas arms was carried out. The 1H NMR and fluorometric spectra confirmed the presence of pyridinoline as the main molecule responsible for collagen crosslinking. Using the amino acid profile and 1H NMR spectra, it was determined that the jumbo squid has a relatively high pyridinoline content. The viscosity properties of this collagen were found to exhibit the behaviour of a pseudoplastic fluid. Finally, under the conditions proposed in this study, the degree of crosslinking of the collagen of the octopus and the jumbo squid did not affect the viscosity properties of the protein.http://www.sciencedirect.com/science/article/pii/S2772502225001428PyridinolineViscosityPseudoplastic fluidFluorometric spectrometryCephalopods |
| spellingShingle | Angel Edgardo Tapia-Vasquez Wilfrido Torres-Arreola Josafat Marina Ezquerra-Brauer Enrique Márquez-Ríos Hisila Santacruz-Ortega Juan Carlos Ramírez-Suárez Guillermina García-Sánchez Guadalupe Miroslava Suárez-Jiménez Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles Applied Food Research Pyridinoline Viscosity Pseudoplastic fluid Fluorometric spectrometry Cephalopods |
| title | Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles |
| title_full | Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles |
| title_fullStr | Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles |
| title_full_unstemmed | Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles |
| title_short | Spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of Octopus vulgaris and Dosidicus gigas arm muscles |
| title_sort | spectrometric determination of the collagen crosslinking degree through pyridinoline identification and evaluation of the viscosity properties of octopus vulgaris and dosidicus gigas arm muscles |
| topic | Pyridinoline Viscosity Pseudoplastic fluid Fluorometric spectrometry Cephalopods |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225001428 |
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