An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia
Trigeminal neuralgia pain remains a challenge to treat. Natural compounds may be promising options for relieving pain. This study was aimed at investigating the effects of aconitine in a rat model of trigeminal neuralgia pain. Infraorbital nerve chronic constriction injury was performed in adult Wis...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6676063 |
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| author | Dilek Çankal Esra Küpeli Akkol Yeliz Kılınç Mert İlhan Raffaele Capasso |
| author_facet | Dilek Çankal Esra Küpeli Akkol Yeliz Kılınç Mert İlhan Raffaele Capasso |
| author_sort | Dilek Çankal |
| collection | DOAJ |
| description | Trigeminal neuralgia pain remains a challenge to treat. Natural compounds may be promising options for relieving pain. This study was aimed at investigating the effects of aconitine in a rat model of trigeminal neuralgia pain. Infraorbital nerve chronic constriction injury was performed in adult Wistar Albino rats. After the neuropathic pain developed, the rats were assigned to one of the treatment groups: carbamazepine 40 or 80 mg/kg; aconitine 0.25, 0.50, or 0.75 mg/kg; or saline injection (control group). Behavioral testing with von Frey filaments and the rotarod test were carried out before the surgical procedure and on the 24th to 29th postoperative days. Following the completion of tests, ipsilateral and contralateral spinal cords were harvested for Western blot analyses to assess NR-1 protein expression. ANOVA followed by Mann-Whitney U test was performed for the statistical analyses. P values of <0.05 were considered significant. Aconitine significantly reduced mechanical sensitivity in a dose-dependent manner. A significant reduction in motor coordination was noted for the higher doses of aconitine which was similar with the 40 and 80 mg/kg doses of carbamazepine. NR-1 expression was reduced in the ipsilateral spinal cord, whereas no significant difference was noted between the groups in the expression of NR-1 in the contralateral spinal cord. Aconitine had a significant pain relieving effect, which was similar to carbamazepine, in a dose-dependent manner. Aconitine may be an alternative pharmacological agent for the control of trigeminal neuralgia pain. |
| format | Article |
| id | doaj-art-ce20e096c95d4dd889394fdb1dc05db4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ce20e096c95d4dd889394fdb1dc05db42025-02-03T01:00:41ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66760636676063An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal NeuralgiaDilek Çankal0Esra Küpeli Akkol1Yeliz Kılınç2Mert İlhan3Raffaele Capasso4Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06490 Ankara, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, TurkeyDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06490 Ankara, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Van Yüzüncü Yıl University, 65080 Van, TurkeyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Portici Naples, ItalyTrigeminal neuralgia pain remains a challenge to treat. Natural compounds may be promising options for relieving pain. This study was aimed at investigating the effects of aconitine in a rat model of trigeminal neuralgia pain. Infraorbital nerve chronic constriction injury was performed in adult Wistar Albino rats. After the neuropathic pain developed, the rats were assigned to one of the treatment groups: carbamazepine 40 or 80 mg/kg; aconitine 0.25, 0.50, or 0.75 mg/kg; or saline injection (control group). Behavioral testing with von Frey filaments and the rotarod test were carried out before the surgical procedure and on the 24th to 29th postoperative days. Following the completion of tests, ipsilateral and contralateral spinal cords were harvested for Western blot analyses to assess NR-1 protein expression. ANOVA followed by Mann-Whitney U test was performed for the statistical analyses. P values of <0.05 were considered significant. Aconitine significantly reduced mechanical sensitivity in a dose-dependent manner. A significant reduction in motor coordination was noted for the higher doses of aconitine which was similar with the 40 and 80 mg/kg doses of carbamazepine. NR-1 expression was reduced in the ipsilateral spinal cord, whereas no significant difference was noted between the groups in the expression of NR-1 in the contralateral spinal cord. Aconitine had a significant pain relieving effect, which was similar to carbamazepine, in a dose-dependent manner. Aconitine may be an alternative pharmacological agent for the control of trigeminal neuralgia pain.http://dx.doi.org/10.1155/2021/6676063 |
| spellingShingle | Dilek Çankal Esra Küpeli Akkol Yeliz Kılınç Mert İlhan Raffaele Capasso An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia Mediators of Inflammation |
| title | An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia |
| title_full | An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia |
| title_fullStr | An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia |
| title_full_unstemmed | An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia |
| title_short | An Effective Phytoconstituent Aconitine: A Realistic Approach for the Treatment of Trigeminal Neuralgia |
| title_sort | effective phytoconstituent aconitine a realistic approach for the treatment of trigeminal neuralgia |
| url | http://dx.doi.org/10.1155/2021/6676063 |
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