Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus
ABSTRACT The genus Sebastes in the North Atlantic comprises of long lived deep‐waters species that have been extensively fished upon, and many stocks are severely depleted across the Atlantic. This is particularly evident for the species Sebastes norvegicus. In recent papers, cryptic species have be...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.70767 |
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| author | Torild Johansen Tanja Hanebrekke Francois Besnier Jon Ivar Westgaard Ingrid Marie Bruvold Geir Dahle Laila Unneland Helle Torp Christensen Atal Saha |
| author_facet | Torild Johansen Tanja Hanebrekke Francois Besnier Jon Ivar Westgaard Ingrid Marie Bruvold Geir Dahle Laila Unneland Helle Torp Christensen Atal Saha |
| author_sort | Torild Johansen |
| collection | DOAJ |
| description | ABSTRACT The genus Sebastes in the North Atlantic comprises of long lived deep‐waters species that have been extensively fished upon, and many stocks are severely depleted across the Atlantic. This is particularly evident for the species Sebastes norvegicus. In recent papers, cryptic species have been indicated within this genus and molecular markers are therefore needed to provide identification for the Sebastes species, including the cryptic species as a basis for advice regarding management and rebuilding of the stocks. A suite of 2800 Single Nucleotide Polymorphism (SNP) markers were identified from ddRAD sequencing data, of which 56 SNPs were organized in two multiplex reactions and tested on 191 Sebastes spp. from different sampling locations from Norway and Greenland. Good‐quality amplification products were successfully obtained from 49 SNP markers for Sebastes species ID, and 3 TaqMan probes were designed to successfully assign S. mentella, S. viviparus, and the two cryptic species S. norvegicus types A and B. A total 47 SNPs were biallelic, with averaged HE per locus ranging between 0.053 and 0.50. This SNP‐based method establishes a foundation for genetically identifying the Northeast Atlantic Sebastes species. The findings presented should be followed by an effort to look for morphological characters to recognize the S. norvegicus cryptic species on site. In general, the SNP markers are a proper tool for monitoring the distribution of the species from a management perspective. |
| format | Article |
| id | doaj-art-ce0a005f78d94d3d9aa8cec8ada412c6 |
| institution | Kabale University |
| issn | 2045-7758 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-ce0a005f78d94d3d9aa8cec8ada412c62025-01-29T05:08:41ZengWileyEcology and Evolution2045-77582025-01-01151n/an/a10.1002/ece3.70767Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicusTorild Johansen0Tanja Hanebrekke1Francois Besnier2Jon Ivar Westgaard3Ingrid Marie Bruvold4Geir Dahle5Laila Unneland6Helle Torp Christensen7Atal Saha8Institute of Marine Research (IMR) Tromsø NorwayInstitute of Marine Research (IMR) Tromsø NorwayInstitute of Marine Research (IMR) Bergen NorwayInstitute of Marine Research (IMR) Tromsø NorwayInstitute of Marine Research (IMR) Tromsø NorwayInstitute of Marine Research (IMR) Bergen NorwayInstitute of Marine Research (IMR) Bergen NorwayGreenland Institute of Natural Resources Nuuk GreenlandCentre for Coastal Research, Department of Natural Sciences University of Agder Kristiansand NorwayABSTRACT The genus Sebastes in the North Atlantic comprises of long lived deep‐waters species that have been extensively fished upon, and many stocks are severely depleted across the Atlantic. This is particularly evident for the species Sebastes norvegicus. In recent papers, cryptic species have been indicated within this genus and molecular markers are therefore needed to provide identification for the Sebastes species, including the cryptic species as a basis for advice regarding management and rebuilding of the stocks. A suite of 2800 Single Nucleotide Polymorphism (SNP) markers were identified from ddRAD sequencing data, of which 56 SNPs were organized in two multiplex reactions and tested on 191 Sebastes spp. from different sampling locations from Norway and Greenland. Good‐quality amplification products were successfully obtained from 49 SNP markers for Sebastes species ID, and 3 TaqMan probes were designed to successfully assign S. mentella, S. viviparus, and the two cryptic species S. norvegicus types A and B. A total 47 SNPs were biallelic, with averaged HE per locus ranging between 0.053 and 0.50. This SNP‐based method establishes a foundation for genetically identifying the Northeast Atlantic Sebastes species. The findings presented should be followed by an effort to look for morphological characters to recognize the S. norvegicus cryptic species on site. In general, the SNP markers are a proper tool for monitoring the distribution of the species from a management perspective.https://doi.org/10.1002/ece3.70767discriminatory SNPsgolden redfishmanagement adviceS. marinus |
| spellingShingle | Torild Johansen Tanja Hanebrekke Francois Besnier Jon Ivar Westgaard Ingrid Marie Bruvold Geir Dahle Laila Unneland Helle Torp Christensen Atal Saha Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus Ecology and Evolution discriminatory SNPs golden redfish management advice S. marinus |
| title | Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus |
| title_full | Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus |
| title_fullStr | Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus |
| title_full_unstemmed | Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus |
| title_short | Development of SNP for Sebastes Species Identification With Special Focus on the Cryptic Species Complex of Sebastes norvegicus |
| title_sort | development of snp for sebastes species identification with special focus on the cryptic species complex of sebastes norvegicus |
| topic | discriminatory SNPs golden redfish management advice S. marinus |
| url | https://doi.org/10.1002/ece3.70767 |
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