Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China

Simplicillium and Leptobacillium, sister genera in the family Cordycipitaceae, exhibit a broad range of hosts or substrates. The identification of two novel species, from Simplicillium and Leptobacillium, was achieved by analysing morphological characteristics and phylogenetic data obtained from six...

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Main Authors: Yingling Lu, Songyu Li, Zuoheng Liu, Jing Zhao, Zhiyong Yu, Zongli Liang, Hailong He, Jianhong Li, Yun Huang, Xinming Li, Hong Yu
Format: Article
Language:English
Published: Pensoft Publishers 2025-03-01
Series:MycoKeys
Online Access:https://mycokeys.pensoft.net/article/140683/download/pdf/
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author Yingling Lu
Songyu Li
Zuoheng Liu
Jing Zhao
Zhiyong Yu
Zongli Liang
Hailong He
Jianhong Li
Yun Huang
Xinming Li
Hong Yu
author_facet Yingling Lu
Songyu Li
Zuoheng Liu
Jing Zhao
Zhiyong Yu
Zongli Liang
Hailong He
Jianhong Li
Yun Huang
Xinming Li
Hong Yu
author_sort Yingling Lu
collection DOAJ
description Simplicillium and Leptobacillium, sister genera in the family Cordycipitaceae, exhibit a broad range of hosts or substrates. The identification of two novel species, from Simplicillium and Leptobacillium, was achieved by analysing morphological characteristics and phylogenetic data obtained from six molecular markers (ITS, nrSSU, nrLSU, tef-1α, rpb1 and rpb2). The two recently documented species are S. puwenense and L. longiphialidum. Morphologically, S. puwenense possessed slender solitary rod-shaped or columnar phialides with elliptical oval or cylindrical conidia forming small spherical heads at the apex of phialides. On the other hand, L. longiphialidum had solitary columnar phialides with elliptic or subspherical apical conidia while other conidia were narrow columnar or fusiform in shape. Phylogenetic analysis revealed that S. puwenense formed an independent branch as a sister species to S. formicae, whereas L. longiphialidum clustered with L. marksiae exhibiting stable topological structure. The Bayesian inference posterior probability and the maximum likelihood bootstrap-ratio provided robust statistical evidence, indicating the presence of two novel species within the genera of Simplicillium and Leptobacillium. The present study contributes to the discovery of species diversity in Simplicillium and Leptobacillium, while also providing a taxonomic foundation for their rational development and sustainable utilisation.
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id doaj-art-ce02ae5a8fb44a59b09cedf3d5f4e6eb
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publishDate 2025-03-01
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spelling doaj-art-ce02ae5a8fb44a59b09cedf3d5f4e6eb2025-08-20T02:50:30ZengPensoft PublishersMycoKeys1314-40492025-03-0111518720810.3897/mycokeys.115.140683140683Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from ChinaYingling Lu0Songyu Li1Zuoheng Liu2Jing Zhao3Zhiyong Yu4Zongli Liang5Hailong He6Jianhong Li7Yun Huang8Xinming Li9Hong Yu10Yunnan UniversityYunnan UniversityYunnan UniversityYunnan UniversityYunnan Jinping Fenshuiling National Nature ReserveYunnan Jinping Fenshuiling National Nature ReserveYunnan Jinping Fenshuiling National Nature ReserveYunnan Jinping Fenshuiling National Nature ReserveYunnan Jinping Fenshuiling National Nature ReserveYunnan Jinping Fenshuiling National Nature ReserveYunnan UniversitySimplicillium and Leptobacillium, sister genera in the family Cordycipitaceae, exhibit a broad range of hosts or substrates. The identification of two novel species, from Simplicillium and Leptobacillium, was achieved by analysing morphological characteristics and phylogenetic data obtained from six molecular markers (ITS, nrSSU, nrLSU, tef-1α, rpb1 and rpb2). The two recently documented species are S. puwenense and L. longiphialidum. Morphologically, S. puwenense possessed slender solitary rod-shaped or columnar phialides with elliptical oval or cylindrical conidia forming small spherical heads at the apex of phialides. On the other hand, L. longiphialidum had solitary columnar phialides with elliptic or subspherical apical conidia while other conidia were narrow columnar or fusiform in shape. Phylogenetic analysis revealed that S. puwenense formed an independent branch as a sister species to S. formicae, whereas L. longiphialidum clustered with L. marksiae exhibiting stable topological structure. The Bayesian inference posterior probability and the maximum likelihood bootstrap-ratio provided robust statistical evidence, indicating the presence of two novel species within the genera of Simplicillium and Leptobacillium. The present study contributes to the discovery of species diversity in Simplicillium and Leptobacillium, while also providing a taxonomic foundation for their rational development and sustainable utilisation.https://mycokeys.pensoft.net/article/140683/download/pdf/
spellingShingle Yingling Lu
Songyu Li
Zuoheng Liu
Jing Zhao
Zhiyong Yu
Zongli Liang
Hailong He
Jianhong Li
Yun Huang
Xinming Li
Hong Yu
Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
MycoKeys
title Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
title_full Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
title_fullStr Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
title_full_unstemmed Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
title_short Morphology and phylogeny of two new species within Cordycipitaceae (Hypocreales) from China
title_sort morphology and phylogeny of two new species within cordycipitaceae hypocreales from china
url https://mycokeys.pensoft.net/article/140683/download/pdf/
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