Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>

Background: Biofilm formation in <i>Helicobacter pylori</i> (<i>H. pylori</i>) helps bacteria survive antibiotic exposure and supports bacterial colonization and persistence in the stomach. Most of the published articles have focused on one aspect of the biofilm. Therefore, w...

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Main Authors: Kartika Afrida Fauzia, Wiwin Is Effendi, Ricky Indra Alfaray, Hoda M. Malaty, Yoshio Yamaoka, Muhammad Mifthussurur
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Antibiotics
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Online Access:https://www.mdpi.com/2079-6382/13/10/976
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author Kartika Afrida Fauzia
Wiwin Is Effendi
Ricky Indra Alfaray
Hoda M. Malaty
Yoshio Yamaoka
Muhammad Mifthussurur
author_facet Kartika Afrida Fauzia
Wiwin Is Effendi
Ricky Indra Alfaray
Hoda M. Malaty
Yoshio Yamaoka
Muhammad Mifthussurur
author_sort Kartika Afrida Fauzia
collection DOAJ
description Background: Biofilm formation in <i>Helicobacter pylori</i> (<i>H. pylori</i>) helps bacteria survive antibiotic exposure and supports bacterial colonization and persistence in the stomach. Most of the published articles have focused on one aspect of the biofilm. Therefore, we conducted the current study to better understand the mechanism of biofilm formation, how the biofilm contributes to antibiotic resistance, and how the biofilm modifies the medication delivery mechanism. Methods: We conducted a literature review analysis of the published articles on the <i>Helicobacter pylori</i> biofilm between 1998 and 2024 from the PubMed database to retrieve eligible articles. After applying the inclusion and exclusion criteria, two hundred and seventy-three articles were eligible for our study. Results: The results showed that biofilm formation starts as adhesion and progresses through micro-colonies, maturation, and dispersion in a planktonic form. Moreover, specific genes modulate each phase of biofilm formation. Few studies have shown that mechanisms, such as quorum sensing and diffusible signal factors, enhance coordination among bacteria when switching from biofilm to planktonic states. Different protein expressions were also observed between planktonic and biofilm strains, and the biofilm architecture was supported by exopolysaccharides, extracellular DNA, and outer membrane vesicles. Conclusions: This infrastructure is responsible for the increased survival of bacteria, especially in harsh environments or in the presence of antibiotics. Therefore, understanding the biofilm formation for <i>H. pylori</i> is crucial. This study illustrates biofilm formation in <i>H. pylori</i> to help improve the treatment of <i>H. pylori</i> infection.
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spelling doaj-art-cdfc87e6cf2041e58edc9a7fdbbf8dca2025-08-20T02:11:04ZengMDPI AGAntibiotics2079-63822024-10-01131097610.3390/antibiotics13100976Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>Kartika Afrida Fauzia0Wiwin Is Effendi1Ricky Indra Alfaray2Hoda M. Malaty3Yoshio Yamaoka4Muhammad Mifthussurur5Research Center for Preclinical and Clinical Medicine, National Research and Innovation Agency, Bogor 16915, IndonesiaDepartment of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, IndonesiaDepartment of Environmental and Preventive Medicine—The Research Center for GLOBAL and LOCAL Infectious Disease (RCGLID), Faculty of Medicine, Oita University, Yufu 879-5593, JapanDepartment of Medicine, Baylor College of Medicine, Houston, TX 77030, USADepartment of Environmental and Preventive Medicine—The Research Center for GLOBAL and LOCAL Infectious Disease (RCGLID), Faculty of Medicine, Oita University, Yufu 879-5593, Japan<i>Helicobacter pylori</i> and Microbiota Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60131, IndonesiaBackground: Biofilm formation in <i>Helicobacter pylori</i> (<i>H. pylori</i>) helps bacteria survive antibiotic exposure and supports bacterial colonization and persistence in the stomach. Most of the published articles have focused on one aspect of the biofilm. Therefore, we conducted the current study to better understand the mechanism of biofilm formation, how the biofilm contributes to antibiotic resistance, and how the biofilm modifies the medication delivery mechanism. Methods: We conducted a literature review analysis of the published articles on the <i>Helicobacter pylori</i> biofilm between 1998 and 2024 from the PubMed database to retrieve eligible articles. After applying the inclusion and exclusion criteria, two hundred and seventy-three articles were eligible for our study. Results: The results showed that biofilm formation starts as adhesion and progresses through micro-colonies, maturation, and dispersion in a planktonic form. Moreover, specific genes modulate each phase of biofilm formation. Few studies have shown that mechanisms, such as quorum sensing and diffusible signal factors, enhance coordination among bacteria when switching from biofilm to planktonic states. Different protein expressions were also observed between planktonic and biofilm strains, and the biofilm architecture was supported by exopolysaccharides, extracellular DNA, and outer membrane vesicles. Conclusions: This infrastructure is responsible for the increased survival of bacteria, especially in harsh environments or in the presence of antibiotics. Therefore, understanding the biofilm formation for <i>H. pylori</i> is crucial. This study illustrates biofilm formation in <i>H. pylori</i> to help improve the treatment of <i>H. pylori</i> infection.https://www.mdpi.com/2079-6382/13/10/976biofilm formation<i>H. pylori</i>infectiontreatmenthuman and health
spellingShingle Kartika Afrida Fauzia
Wiwin Is Effendi
Ricky Indra Alfaray
Hoda M. Malaty
Yoshio Yamaoka
Muhammad Mifthussurur
Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
Antibiotics
biofilm formation
<i>H. pylori</i>
infection
treatment
human and health
title Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
title_full Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
title_fullStr Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
title_full_unstemmed Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
title_short Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
title_sort molecular mechanisms of biofilm formation in i helicobacter pylori i
topic biofilm formation
<i>H. pylori</i>
infection
treatment
human and health
url https://www.mdpi.com/2079-6382/13/10/976
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