Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua)
There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction meth...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302200221X |
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| author | Zhen Yuan Meilian Yang Dongyang Zhu Di Wu Shuzhen Cheng Chao Wu Hesham R. El-Seedi Ming Du |
| author_facet | Zhen Yuan Meilian Yang Dongyang Zhu Di Wu Shuzhen Cheng Chao Wu Hesham R. El-Seedi Ming Du |
| author_sort | Zhen Yuan |
| collection | DOAJ |
| description | There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction method, the isolated ethanol-soluble cod peptides (CP-ES) were investigated and their immunomodulatory activity was verified. Additionally, CP-ES showed lower molecular weight and more hydrophobic amino acids. CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice, suggesting that CP-ES may regulate adaptive immunity. It promoted the release of NO and the expression of iNOS, TNF-α, IL-6 and IL-1β genes in macrophages, suggesting that CP-ES may regulate innate immunity. CP-ES could promote the expression of TLR2 gene, and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES. These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines. |
| format | Article |
| id | doaj-art-cdf4e6efc8ac4f95abfd84371bdf3870 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-cdf4e6efc8ac4f95abfd84371bdf38702025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411921203Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua)Zhen Yuan0Meilian Yang1Dongyang Zhu2Di Wu3Shuzhen Cheng4Chao Wu5Hesham R. El-Seedi6Ming Du7School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, ChinaPharmacognosy Group, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala 75123, SwedenSchool of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China; Corresponding author at: School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction method, the isolated ethanol-soluble cod peptides (CP-ES) were investigated and their immunomodulatory activity was verified. Additionally, CP-ES showed lower molecular weight and more hydrophobic amino acids. CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice, suggesting that CP-ES may regulate adaptive immunity. It promoted the release of NO and the expression of iNOS, TNF-α, IL-6 and IL-1β genes in macrophages, suggesting that CP-ES may regulate innate immunity. CP-ES could promote the expression of TLR2 gene, and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES. These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines.http://www.sciencedirect.com/science/article/pii/S221345302200221XGadus morhuaImmunomodulationPeptidesTLR2Molecular docking |
| spellingShingle | Zhen Yuan Meilian Yang Dongyang Zhu Di Wu Shuzhen Cheng Chao Wu Hesham R. El-Seedi Ming Du Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) Food Science and Human Wellness Gadus morhua Immunomodulation Peptides TLR2 Molecular docking |
| title | Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) |
| title_full | Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) |
| title_fullStr | Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) |
| title_full_unstemmed | Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) |
| title_short | Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua) |
| title_sort | immunomodulatory effect of ethanol soluble oligopeptides from atlantic cod gadus morhua |
| topic | Gadus morhua Immunomodulation Peptides TLR2 Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S221345302200221X |
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