Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin
Mycotoxin contamination in feedstuffs is a worldwide problem that causes serious health issues both in humans and animals, and it contributes to serious economic losses. Deoxynivalenol (DON) and T-2 toxin (T-2) are major trichothecene mycotoxins and are known to challenge mainly intestinal barrier f...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8880651 |
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| author | Judit Mercédesz Pomothy Réka Fanni Barna Erzsébet Anna Pászti Ákos Babiczky Áron Szóládi Ákos Jerzsele Erzsébet Pásztiné Gere |
| author_facet | Judit Mercédesz Pomothy Réka Fanni Barna Erzsébet Anna Pászti Ákos Babiczky Áron Szóládi Ákos Jerzsele Erzsébet Pásztiné Gere |
| author_sort | Judit Mercédesz Pomothy |
| collection | DOAJ |
| description | Mycotoxin contamination in feedstuffs is a worldwide problem that causes serious health issues both in humans and animals, and it contributes to serious economic losses. Deoxynivalenol (DON) and T-2 toxin (T-2) are major trichothecene mycotoxins and are known to challenge mainly intestinal barrier functions. Polyphenolic rosmarinic acid (RA) appeared to have antioxidant and anti-inflammatory properties in vitro. The aim of this study was to investigate protective effects of RA against DON and T-2 or combined mycotoxin-induced intestinal damage in nontumorigenic porcine cell line, IPEC-J2. It was ascertained that simultaneous treatment of DON and T-2 (DT2: 1 μmol/L DON+5 nmol/L T−2) for 48 h and 72 h reduced transepithelial electrical resistance of cell monolayer, which was restored by 50 μmol/L RA application. It was also found that DT2 for 48 h and 72 h could induce oxidative stress and elevate interleukin-6 (IL-6) and interleukin-8 (IL-8) levels significantly, which were alleviated by the administration of RA. DT2 administration contributed to the redistribution of claudin-1; however, occludin membranous localization was not altered by combined mycotoxin treatment. In conclusion, beneficial effect of RA was exerted on DT2-deteriorated cell monolayer integrity and on the perturbated redox status of IPEC-J2 cells. |
| format | Article |
| id | doaj-art-cdd03d5a987d4e55a7aacff8bca2104e |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cdd03d5a987d4e55a7aacff8bca2104e2025-08-20T03:19:39ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/88806518880651Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 ToxinJudit Mercédesz Pomothy0Réka Fanni Barna1Erzsébet Anna Pászti2Ákos Babiczky3Áron Szóládi4Ákos Jerzsele5Erzsébet Pásztiné Gere6Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryNeuronal Networks and Behaviour Research Group, Research Centre for Natural Sciences, H-1117 Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, HungaryMycotoxin contamination in feedstuffs is a worldwide problem that causes serious health issues both in humans and animals, and it contributes to serious economic losses. Deoxynivalenol (DON) and T-2 toxin (T-2) are major trichothecene mycotoxins and are known to challenge mainly intestinal barrier functions. Polyphenolic rosmarinic acid (RA) appeared to have antioxidant and anti-inflammatory properties in vitro. The aim of this study was to investigate protective effects of RA against DON and T-2 or combined mycotoxin-induced intestinal damage in nontumorigenic porcine cell line, IPEC-J2. It was ascertained that simultaneous treatment of DON and T-2 (DT2: 1 μmol/L DON+5 nmol/L T−2) for 48 h and 72 h reduced transepithelial electrical resistance of cell monolayer, which was restored by 50 μmol/L RA application. It was also found that DT2 for 48 h and 72 h could induce oxidative stress and elevate interleukin-6 (IL-6) and interleukin-8 (IL-8) levels significantly, which were alleviated by the administration of RA. DT2 administration contributed to the redistribution of claudin-1; however, occludin membranous localization was not altered by combined mycotoxin treatment. In conclusion, beneficial effect of RA was exerted on DT2-deteriorated cell monolayer integrity and on the perturbated redox status of IPEC-J2 cells.http://dx.doi.org/10.1155/2020/8880651 |
| spellingShingle | Judit Mercédesz Pomothy Réka Fanni Barna Erzsébet Anna Pászti Ákos Babiczky Áron Szóládi Ákos Jerzsele Erzsébet Pásztiné Gere Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin Mediators of Inflammation |
| title | Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin |
| title_full | Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin |
| title_fullStr | Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin |
| title_full_unstemmed | Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin |
| title_short | Beneficial Effects of Rosmarinic Acid on IPEC-J2 Cells Exposed to the Combination of Deoxynivalenol and T-2 Toxin |
| title_sort | beneficial effects of rosmarinic acid on ipec j2 cells exposed to the combination of deoxynivalenol and t 2 toxin |
| url | http://dx.doi.org/10.1155/2020/8880651 |
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