Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients
Background. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to cont...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/1061276 |
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| author | Marcella Nebbioso Marta Sacchetti Guia Bianchi Anna Maria Zicari Marzia Duse Paola Del Regno Alessandro Lambiase |
| author_facet | Marcella Nebbioso Marta Sacchetti Guia Bianchi Anna Maria Zicari Marzia Duse Paola Del Regno Alessandro Lambiase |
| author_sort | Marcella Nebbioso |
| collection | DOAJ |
| description | Background. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose.To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods. Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results. Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions. The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy. |
| format | Article |
| id | doaj-art-cdc555c1955849bf84fc9c16fddf01bb |
| institution | DOAJ |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-cdc555c1955849bf84fc9c16fddf01bb2025-08-20T03:19:41ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/10612761061276Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis PatientsMarcella Nebbioso0Marta Sacchetti1Guia Bianchi2Anna Maria Zicari3Marzia Duse4Paola Del Regno5Alessandro Lambiase6Department of Sense Organs, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Sense Organs, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Sense Organs, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Pediatrics, Faculty of Medicine and Odontology, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Pediatrics, Faculty of Medicine and Odontology, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Sense Organs, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyDepartment of Sense Organs, Sapienza University of Rome, p. le A. Moro 5, 00185 Rome, ItalyBackground. Vernal keratoconjunctivitis (VKC) is a rare ocular surface inflammatory disease that affects mainly boys in the first decade of life. Clinical observations show that it generally regresses spontaneously with the onset of puberty, but therapeutic measures must be taken before then to control the course of the disease. Purpose.To evaluate the role of the lacrimal mucous component in VKC patients and compare tear ferning test (TFT) modifications, MUC5AC levels in tears, and density of conjunctival goblet cells to clinical characteristics before and after treatment with cyclosporine A (CY) in eye drops. Methods. Forty-seven patients affected by VKC and 30 healthy subjects aged between 3 and 16 years of life were enrolled. All individuals were submitted to complete eye examination and skin prick test (SPT) for the most common allergens. Then, they were subjected to collection of the tears and to impression cytology to evaluate TFT, MUC5AC levels, and conjunctival goblet cell density, before and after treatment with CY in eye drops. Results. Comparing the VKC group vs. the control group at baseline, a significant alteration in the degree of the ferns was found, indicating a pathological condition of the lacrimal mucous layer. In addition, an increased number of goblet cells were observed in the patients. The concentration of lacrimal secretory mucins (MUC5AC) did not show significant differences between the 2 groups. Patients treated with CY have reported improvements of some signs and symptoms of disease activity, including TFT, and a tendency of conjunctival goblet cell density to normalise. Conclusions. The results obtained demonstrated for the first time a significant alteration of the lacrimal mucin component evaluated in the VKC group, and an improvement of the latter after CY therapy.http://dx.doi.org/10.1155/2018/1061276 |
| spellingShingle | Marcella Nebbioso Marta Sacchetti Guia Bianchi Anna Maria Zicari Marzia Duse Paola Del Regno Alessandro Lambiase Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients Journal of Ophthalmology |
| title | Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients |
| title_full | Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients |
| title_fullStr | Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients |
| title_full_unstemmed | Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients |
| title_short | Tear Ferning Test and Pathological Effects on Ocular Surface before and after Topical Cyclosporine in Vernal Keratoconjunctivitis Patients |
| title_sort | tear ferning test and pathological effects on ocular surface before and after topical cyclosporine in vernal keratoconjunctivitis patients |
| url | http://dx.doi.org/10.1155/2018/1061276 |
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