Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China
Cipangopaludina chinensis is a unique species with both medicinal and culinary applications, offering significant economic and nutritional benefits in China. However, the large amount of fishing and the invasion of other species have severely impacted the natural resources of C. chinensis in China....
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000316 |
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| author | Xiao-kai Wei Kang-qi Zhou Xin-xi Zou Xue-ling Zhang Yu-Sen Li Hui Luo Yin Huang Xue-song Du Jun-qi Qin Zhong Chen Yong Lin Xian-hui Pan Hua Ye |
| author_facet | Xiao-kai Wei Kang-qi Zhou Xin-xi Zou Xue-ling Zhang Yu-Sen Li Hui Luo Yin Huang Xue-song Du Jun-qi Qin Zhong Chen Yong Lin Xian-hui Pan Hua Ye |
| author_sort | Xiao-kai Wei |
| collection | DOAJ |
| description | Cipangopaludina chinensis is a unique species with both medicinal and culinary applications, offering significant economic and nutritional benefits in China. However, the large amount of fishing and the invasion of other species have severely impacted the natural resources of C. chinensis in China. Moreover, the molecular-level population structure of C. chinensis remains poor. In this study, the genetic diversity and population structure of 10 different geographical populations of C. chinensis from Guangxi, China, were analyzed using microsatellite DNA markers. The results showed that a total of 176 alleles in the populations were detected by 12 microsatellite markers. The polymorphism information content (PIC) was 0.523–0.918, indicating that these markers were highly polymorphic (PIC > 0.5). The observed heterozygosity (Ho) and expected heterozygosity (He) of the 10 populations were 0.156 (RS)-0.533 (NN and WZ) and 0.186 (RS)-0.702 (QZ), respectively. This indicated that the genetic diversity of NN, RA, and WZ populations was relatively high, while the genetic diversity of HC, NP, and RS populations was low. The genetic diversity of the remaining four populations was at a medium level. AMOVA analysis showed that high genetic differentiation was found among the populations (Fst=0.313, P < 0.001), and the genetic variation within populations (69 %) was greater than among populations (31 %). The genetic structure results revealed that all populations could be divided into two genetic clusters, which was confirmed by the UPGMA tree constructed based on Nei’s unbiased genetic distance and PCoA analysis. These findings provide important genetic information for C. chinensis and a theoretical basis for future protection and selective breeding between different geographical populations. |
| format | Article |
| id | doaj-art-cdc020b875374431837303277436fbf3 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-cdc020b875374431837303277436fbf32025-02-06T05:12:25ZengElsevierAquaculture Reports2352-51342025-03-0140102645Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, ChinaXiao-kai Wei0Kang-qi Zhou1Xin-xi Zou2Xue-ling Zhang3Yu-Sen Li4Hui Luo5Yin Huang6Xue-song Du7Jun-qi Qin8Zhong Chen9Yong Lin10Xian-hui Pan11Hua Ye12Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, China; Correspondence to: Room 116, Scientific Research Building No. 1, Qingshan Road No. 8, Nanning, Guangxi 530021, China.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Aquatic Breeding Base, Guangxi Academy of Fishery Sciences, Nanning 530021, China; Correspondence to: Room 118, Scientific Research Building No. 1, Qingshan Road No. 8, Nanning, Guangxi 530021, China.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, College of Fisheries, Southwest University, Chongqing 402460, China; Correspondence to: Room 114, Teaching Building No. 1, Xueyuan Road No. 160, Chongqing 530021, China.Cipangopaludina chinensis is a unique species with both medicinal and culinary applications, offering significant economic and nutritional benefits in China. However, the large amount of fishing and the invasion of other species have severely impacted the natural resources of C. chinensis in China. Moreover, the molecular-level population structure of C. chinensis remains poor. In this study, the genetic diversity and population structure of 10 different geographical populations of C. chinensis from Guangxi, China, were analyzed using microsatellite DNA markers. The results showed that a total of 176 alleles in the populations were detected by 12 microsatellite markers. The polymorphism information content (PIC) was 0.523–0.918, indicating that these markers were highly polymorphic (PIC > 0.5). The observed heterozygosity (Ho) and expected heterozygosity (He) of the 10 populations were 0.156 (RS)-0.533 (NN and WZ) and 0.186 (RS)-0.702 (QZ), respectively. This indicated that the genetic diversity of NN, RA, and WZ populations was relatively high, while the genetic diversity of HC, NP, and RS populations was low. The genetic diversity of the remaining four populations was at a medium level. AMOVA analysis showed that high genetic differentiation was found among the populations (Fst=0.313, P < 0.001), and the genetic variation within populations (69 %) was greater than among populations (31 %). The genetic structure results revealed that all populations could be divided into two genetic clusters, which was confirmed by the UPGMA tree constructed based on Nei’s unbiased genetic distance and PCoA analysis. These findings provide important genetic information for C. chinensis and a theoretical basis for future protection and selective breeding between different geographical populations.http://www.sciencedirect.com/science/article/pii/S2352513425000316Cipangopaludina chinensisMicrosatellite markersGenetic diversityPopulation structure |
| spellingShingle | Xiao-kai Wei Kang-qi Zhou Xin-xi Zou Xue-ling Zhang Yu-Sen Li Hui Luo Yin Huang Xue-song Du Jun-qi Qin Zhong Chen Yong Lin Xian-hui Pan Hua Ye Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China Aquaculture Reports Cipangopaludina chinensis Microsatellite markers Genetic diversity Population structure |
| title | Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China |
| title_full | Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China |
| title_fullStr | Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China |
| title_full_unstemmed | Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China |
| title_short | Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China |
| title_sort | microsatellite analyses reveal genetic diversity and population structure of cipangopaludina chinensis in guangxi china |
| topic | Cipangopaludina chinensis Microsatellite markers Genetic diversity Population structure |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000316 |
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