Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities
Abstract Nanotechnology has witnessed remarkable advancements in recent years, capturing considerable attention in diverse biomedical applications. Using the green precipitation method, this study aims to synthesize and characterize chitosan/polyvinyl alcohol-copper oxide nanocomposites (CS/PVA/CuON...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-87932-6 |
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| author | Manickam Rajkumar S. I. Davis Presley Prabha Govindaraj Dharmalingam Kirubakaran Farha Farahim Talat Ali Mohd Shkir Sellapillai Latha |
| author_facet | Manickam Rajkumar S. I. Davis Presley Prabha Govindaraj Dharmalingam Kirubakaran Farha Farahim Talat Ali Mohd Shkir Sellapillai Latha |
| author_sort | Manickam Rajkumar |
| collection | DOAJ |
| description | Abstract Nanotechnology has witnessed remarkable advancements in recent years, capturing considerable attention in diverse biomedical applications. Using the green precipitation method, this study aims to synthesize and characterize chitosan/polyvinyl alcohol-copper oxide nanocomposites (CS/PVA/CuONCs) using Anacardium occidentale plant fruit extract. The CS/PVA/CuONCs were further evaluated in antioxidant, antibacterial and biological activities. In our study results, UV–Vis spectrum analysis of CS/PVA/CuONCs revealed a peak at 430 nm. FTIR analyses confirmed the presence of different functional groups, while the XRD study confirmed the crystalline structure of the synthesized nanocomposites. FESEM-EDAX analysis demonstrated that the CS/PVA/CuONCs exhibited a spherical and rod-like shape, with an average particle size of 48.6 to 96.2 nm. Notably, CS/PVA/CuONCs exhibited higher antioxidant activity, as evidenced by their ABTS activity (83.79 ± 1.57%) and SOD activity (86.17 ± 1.28%). In antibacterial assays, CS/PVA/CuONCs demonstrated inhibition in Escherichia coli at 20.52 ± 0.85 mm and Bacillus subtilis at 19.64 ± 0.87 mm, displaying a zone of inhibition. The CS/PVA/CuONCs exhibited excellent anti-inflammatory potency against COX-1 (67.10 ± 0.58%) and COX-2 (76.39 ± 0.65%). The antidiabetic assay revealed excellent α-amylase inhibition (80.25 ± 1.29%) and α-glucosidase inhibition (84.74 ± 1.42%) activities. Anti-cholinergic activity of AChE was 65.35 ± 0.98% and BuChE was 82.46 ± 1.15% are observed. CS/PVA/CuONCs was shown to have strong cytotoxicity against MCF-7 cell lines. It also had the highest cell viability inhibition, at 13.66 ± 0.58%. The hemolysis activity was found to be 5.38 ± 0.34%. Overall, the study demonstrated that CS/PVA/CuONCs possess remarkably excellent biological activities. |
| format | Article |
| id | doaj-art-cdad7392d94140599e98697c08788547 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-cdad7392d94140599e98697c087885472025-02-02T12:17:08ZengNature PortfolioScientific Reports2045-23222025-01-0115112010.1038/s41598-025-87932-6Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activitiesManickam Rajkumar0S. I. Davis Presley1Prabha Govindaraj2Dharmalingam Kirubakaran3Farha Farahim4Talat Ali5Mohd Shkir6Sellapillai Latha7Department of Chemistry, Sri Sivasubramaniya Nadar College of EngineeringDepartment of Chemistry, Sri Sivasubramaniya Nadar College of EngineeringDepartment of Chemistry, St. Joseph’s Institute of TechnologyDepartment of Pharmacology, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha UniversityDepartment of Community, Psychiatric and Mental Health Nursing, King Khalid UniversityDepartment of Basic Medical Science, King Khalid UniversityDepartment of Physics, College of Science, King Khalid UniversityDepartment of Biotechnology, Vinayaka Mission’s Kirupanada Variyar Engineering CollegeAbstract Nanotechnology has witnessed remarkable advancements in recent years, capturing considerable attention in diverse biomedical applications. Using the green precipitation method, this study aims to synthesize and characterize chitosan/polyvinyl alcohol-copper oxide nanocomposites (CS/PVA/CuONCs) using Anacardium occidentale plant fruit extract. The CS/PVA/CuONCs were further evaluated in antioxidant, antibacterial and biological activities. In our study results, UV–Vis spectrum analysis of CS/PVA/CuONCs revealed a peak at 430 nm. FTIR analyses confirmed the presence of different functional groups, while the XRD study confirmed the crystalline structure of the synthesized nanocomposites. FESEM-EDAX analysis demonstrated that the CS/PVA/CuONCs exhibited a spherical and rod-like shape, with an average particle size of 48.6 to 96.2 nm. Notably, CS/PVA/CuONCs exhibited higher antioxidant activity, as evidenced by their ABTS activity (83.79 ± 1.57%) and SOD activity (86.17 ± 1.28%). In antibacterial assays, CS/PVA/CuONCs demonstrated inhibition in Escherichia coli at 20.52 ± 0.85 mm and Bacillus subtilis at 19.64 ± 0.87 mm, displaying a zone of inhibition. The CS/PVA/CuONCs exhibited excellent anti-inflammatory potency against COX-1 (67.10 ± 0.58%) and COX-2 (76.39 ± 0.65%). The antidiabetic assay revealed excellent α-amylase inhibition (80.25 ± 1.29%) and α-glucosidase inhibition (84.74 ± 1.42%) activities. Anti-cholinergic activity of AChE was 65.35 ± 0.98% and BuChE was 82.46 ± 1.15% are observed. CS/PVA/CuONCs was shown to have strong cytotoxicity against MCF-7 cell lines. It also had the highest cell viability inhibition, at 13.66 ± 0.58%. The hemolysis activity was found to be 5.38 ± 0.34%. Overall, the study demonstrated that CS/PVA/CuONCs possess remarkably excellent biological activities.https://doi.org/10.1038/s41598-025-87932-6BiomaterialAntioxidantsAntibacterialAnti-diabeticMedicinal plant |
| spellingShingle | Manickam Rajkumar S. I. Davis Presley Prabha Govindaraj Dharmalingam Kirubakaran Farha Farahim Talat Ali Mohd Shkir Sellapillai Latha Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities Scientific Reports Biomaterial Antioxidants Antibacterial Anti-diabetic Medicinal plant |
| title | Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities |
| title_full | Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities |
| title_fullStr | Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities |
| title_full_unstemmed | Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities |
| title_short | Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities |
| title_sort | synthesis of chitosan pva copper oxide nanocomposite using anacardium occidentale extract and evaluating its antioxidant antibacterial anti inflammatory and cytotoxic activities |
| topic | Biomaterial Antioxidants Antibacterial Anti-diabetic Medicinal plant |
| url | https://doi.org/10.1038/s41598-025-87932-6 |
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