Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer
Recent studies have shown that polymorphisms in leptin and leptin receptor genes are associated with increased risk for breast cancer. This study aimed at investigating -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene in patients with breast cancer. The study incl...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2015/132720 |
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| _version_ | 1832562286888222720 |
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| author | Reza Mahmoudi Bahareh Noori Alavicheh Mohammad Amin Nazer Mozaffari Mohammad Fararouei Mohsen Nikseresht |
| author_facet | Reza Mahmoudi Bahareh Noori Alavicheh Mohammad Amin Nazer Mozaffari Mohammad Fararouei Mohsen Nikseresht |
| author_sort | Reza Mahmoudi |
| collection | DOAJ |
| description | Recent studies have shown that polymorphisms in leptin and leptin receptor genes are associated with increased risk for breast cancer. This study aimed at investigating -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene in patients with breast cancer. The study included 45 women with breast cancer and 41 healthy women. PCR-RFLP was used to determine the genotype of the subjects in terms of -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene. Serum levels of leptin were also measured by ELISA. For -2548 G/A polymorphism, the genotypes were homozygous AA (OR = 1.13; p=0.8) and heterozygous GA (OR = 0.41; p=0.2) and for Q223R polymorphism, the genotypes were homozygous RR (OR = 6.7; p=0.09) and heterozygous QR (OR = 8.3; p=0.06). The mean serum level of leptin was 33.22 ± 21.35 ng/mL in patients and 29.49 ± 23.27 ng/mL in the normal participants (p=0.3). Although, despite the magnitude of the associations, the results suggested no statistically significant contribution of -2548 G/A polymorphism (in leptin gene), Q223R polymorphism (in leptin receptor gene), and serum leptin levels in predicting the risk of breast cancer, further studies with larger sample size are suggested. |
| format | Article |
| id | doaj-art-cda694d2958f45f9a3a9ecc988150e53 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-cda694d2958f45f9a3a9ecc988150e532025-02-03T01:23:02ZengWileyInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/132720132720Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast CancerReza Mahmoudi0Bahareh Noori Alavicheh1Mohammad Amin Nazer Mozaffari2Mohammad Fararouei3Mohsen Nikseresht4Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranStudents Research Committee, Yasuj University of Medical Sciences, Yasuj, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranHIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, IranCellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IranRecent studies have shown that polymorphisms in leptin and leptin receptor genes are associated with increased risk for breast cancer. This study aimed at investigating -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene in patients with breast cancer. The study included 45 women with breast cancer and 41 healthy women. PCR-RFLP was used to determine the genotype of the subjects in terms of -2548 G/A polymorphism in leptin gene and Q223R polymorphism in leptin receptor gene. Serum levels of leptin were also measured by ELISA. For -2548 G/A polymorphism, the genotypes were homozygous AA (OR = 1.13; p=0.8) and heterozygous GA (OR = 0.41; p=0.2) and for Q223R polymorphism, the genotypes were homozygous RR (OR = 6.7; p=0.09) and heterozygous QR (OR = 8.3; p=0.06). The mean serum level of leptin was 33.22 ± 21.35 ng/mL in patients and 29.49 ± 23.27 ng/mL in the normal participants (p=0.3). Although, despite the magnitude of the associations, the results suggested no statistically significant contribution of -2548 G/A polymorphism (in leptin gene), Q223R polymorphism (in leptin receptor gene), and serum leptin levels in predicting the risk of breast cancer, further studies with larger sample size are suggested.http://dx.doi.org/10.1155/2015/132720 |
| spellingShingle | Reza Mahmoudi Bahareh Noori Alavicheh Mohammad Amin Nazer Mozaffari Mohammad Fararouei Mohsen Nikseresht Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer International Journal of Genomics |
| title | Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer |
| title_full | Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer |
| title_fullStr | Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer |
| title_full_unstemmed | Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer |
| title_short | Polymorphisms of Leptin (-2548 G/A) and Leptin Receptor (Q223R) Genes in Iranian Women with Breast Cancer |
| title_sort | polymorphisms of leptin 2548 g a and leptin receptor q223r genes in iranian women with breast cancer |
| url | http://dx.doi.org/10.1155/2015/132720 |
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