The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study
Background: Over the past decade, numerous studies have been conducted to determine the role of homocysteineand methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms in the pathogenesis of polycystic ovary syndrome(PCOS), yet the results are inconsistent. The aim of the current study was to...
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Royan Institute (ACECR), Tehran
2025-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | https://www.ijfs.ir/article_714915_31cadc6e437b6c510c0497094c8b39ee.pdf |
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| author | Elene Asanidze Zurab R. Tsetskhladze Jenaro Kristesashvili Marina Pirtskhalava Manana Urjumelashvili Shorena Tchiokadze Malkhaz Vakhania Nino Parunashvili Aleksandre Asanidze |
| author_facet | Elene Asanidze Zurab R. Tsetskhladze Jenaro Kristesashvili Marina Pirtskhalava Manana Urjumelashvili Shorena Tchiokadze Malkhaz Vakhania Nino Parunashvili Aleksandre Asanidze |
| author_sort | Elene Asanidze |
| collection | DOAJ |
| description | Background: Over the past decade, numerous studies have been conducted to determine the role of homocysteineand methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms in the pathogenesis of polycystic ovary syndrome(PCOS), yet the results are inconsistent. The aim of the current study was to determine the association betweenhomocysteine levels (Hcy), MTHFR C677T and A1298C polymorphisms, and pregnancy outcomes in Georgianwomen with PCOS.Materials and Methods: This case-control study included 177 female participants, of which 96 women werediagnosed with PCOS, and 81 age-matched women were without PCOS. Participants were divided into fourgroups; group I: 59 PCOS patients with history of recurrent pregnancy loss (RPL), group II: 37 PCOS patientswith live birth in history and without RPL, group III: 39 women with RPL, without PCOS, group IV: controls,42 women with live birth in history, without RPL and PCOS. These groups were compared based on their serumHcy and the presence of two common single nucleotide polymorphisms (SNPs) in the MTHFR gene: C677T andA1298C.Results: The mean Hcy, frequency of hyperhomocysteinemia (Hhcy) and the prevalence of C677T and A1298Cpolymorphisms in MTHFR gene in PCOS patients were significantly higher than those without PCOS (P<0.05).Group I (PCOS with RPL) showed significantly elevated Hcy (13.7 ± 2.7) compared to group II (10.3 ± 2.6),group III (11.5 ± 2.3), and group IV (7.3 ± 2.2), P<0.001. In group I, the frequencies of the C677T-CT, A1298CACgenotypes, and the compound heterozygous of C677T-CT/A1298C-AC were significantly higher than in theother groups (P<0.05). The prevalence of MTHFR A1298C (CC) was significantly higher in group II (PCOSpatients with live birth) than in other comparison groups (P<0.05).Conclusion: The study reveals a significant correlation between hyperhomocysteinemia, MTHFR polymorphisms(C677T and A1298C), and PCOS, impacting pregnancy outcomes. |
| format | Article |
| id | doaj-art-cd7ed51d75304c85b06eb24123f5f852 |
| institution | Kabale University |
| issn | 2008-076X 2008-0778 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
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| series | International Journal of Fertility and Sterility |
| spelling | doaj-art-cd7ed51d75304c85b06eb24123f5f8522025-01-18T09:23:38ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782025-01-01191505710.22074/ijfs.2024.2008377.1504714915The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control StudyElene Asanidze0Zurab R. Tsetskhladze1Jenaro Kristesashvili2Marina Pirtskhalava3Manana Urjumelashvili4Shorena Tchiokadze5Malkhaz Vakhania6Nino Parunashvili7Aleksandre Asanidze8Department of Gynecology, Faculty of Medicine, University Geomedi, Tbilisi, GeorgiaDepartment of Gynecology, Faculty of Medicine, University Geomedi, Tbilisi, GeorgiaDepartment of Gynecology, Faculty of Medicine, Ivane Javakhishvili Tbilisi State University, Tbilisi, GeorgiaDepartment of Gynecology, Faculty of Medicine, University Geomedi, Tbilisi, GeorgiaDepartment of Gynecology, Faculty of Medicine, Ivane Javakhishvili Tbilisi State University, Tbilisi, GeorgiaDepartment of Medical Faculty of David Tvildiani Medical University, Tbilisi, GeorgiaDepartment of Gynecology, Faculty of Medicine, University Geomedi, Tbilisi, GeorgiaUniversity of Toronto, Faculty of Medicine, Physician Assistant Program, Toronto, CanadaDepartment of General Surgery, Faculty of Medicine, Tbilisi State Medical University, Tbilisi, GeorgiaBackground: Over the past decade, numerous studies have been conducted to determine the role of homocysteineand methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms in the pathogenesis of polycystic ovary syndrome(PCOS), yet the results are inconsistent. The aim of the current study was to determine the association betweenhomocysteine levels (Hcy), MTHFR C677T and A1298C polymorphisms, and pregnancy outcomes in Georgianwomen with PCOS.Materials and Methods: This case-control study included 177 female participants, of which 96 women werediagnosed with PCOS, and 81 age-matched women were without PCOS. Participants were divided into fourgroups; group I: 59 PCOS patients with history of recurrent pregnancy loss (RPL), group II: 37 PCOS patientswith live birth in history and without RPL, group III: 39 women with RPL, without PCOS, group IV: controls,42 women with live birth in history, without RPL and PCOS. These groups were compared based on their serumHcy and the presence of two common single nucleotide polymorphisms (SNPs) in the MTHFR gene: C677T andA1298C.Results: The mean Hcy, frequency of hyperhomocysteinemia (Hhcy) and the prevalence of C677T and A1298Cpolymorphisms in MTHFR gene in PCOS patients were significantly higher than those without PCOS (P<0.05).Group I (PCOS with RPL) showed significantly elevated Hcy (13.7 ± 2.7) compared to group II (10.3 ± 2.6),group III (11.5 ± 2.3), and group IV (7.3 ± 2.2), P<0.001. In group I, the frequencies of the C677T-CT, A1298CACgenotypes, and the compound heterozygous of C677T-CT/A1298C-AC were significantly higher than in theother groups (P<0.05). The prevalence of MTHFR A1298C (CC) was significantly higher in group II (PCOSpatients with live birth) than in other comparison groups (P<0.05).Conclusion: The study reveals a significant correlation between hyperhomocysteinemia, MTHFR polymorphisms(C677T and A1298C), and PCOS, impacting pregnancy outcomes.https://www.ijfs.ir/article_714915_31cadc6e437b6c510c0497094c8b39ee.pdfhomocysteinehyperhomocysteinemiamthfrpolycystic ovary syndromerecurrent pregnancy loss |
| spellingShingle | Elene Asanidze Zurab R. Tsetskhladze Jenaro Kristesashvili Marina Pirtskhalava Manana Urjumelashvili Shorena Tchiokadze Malkhaz Vakhania Nino Parunashvili Aleksandre Asanidze The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study International Journal of Fertility and Sterility homocysteine hyperhomocysteinemia mthfr polycystic ovary syndrome recurrent pregnancy loss |
| title | The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study |
| title_full | The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study |
| title_fullStr | The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study |
| title_full_unstemmed | The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study |
| title_short | The Relationship between Homocysteine Levels, MTHFR C677T and A1298C Polymorphism, and Pregnancy Outcomes in Georgian Women with Polycystic Ovary Syndrome: A Case-Control Study |
| title_sort | relationship between homocysteine levels mthfr c677t and a1298c polymorphism and pregnancy outcomes in georgian women with polycystic ovary syndrome a case control study |
| topic | homocysteine hyperhomocysteinemia mthfr polycystic ovary syndrome recurrent pregnancy loss |
| url | https://www.ijfs.ir/article_714915_31cadc6e437b6c510c0497094c8b39ee.pdf |
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