DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most devastating cancer types despite the improvement of modern medicine. In our present study, we found that dickkopf-related protein 2 (DKK2) shares a higher expression in PDAC compared with adjacent pancreas tissue in tissue microarray. I...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/8656282 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563663936946176 |
|---|---|
| author | Jianyu Yang Yongsheng Jiang Ruizhe He Wei Liu Minwei Yang Lingye Tao Xueliang Fu Yang Shen Jiao Li Dejun Liu Yanmiao Huo Junfeng Zhang Rong Hua Zhigang Zhang Yongwei Sun |
| author_facet | Jianyu Yang Yongsheng Jiang Ruizhe He Wei Liu Minwei Yang Lingye Tao Xueliang Fu Yang Shen Jiao Li Dejun Liu Yanmiao Huo Junfeng Zhang Rong Hua Zhigang Zhang Yongwei Sun |
| author_sort | Jianyu Yang |
| collection | DOAJ |
| description | Pancreatic ductal adenocarcinoma (PDAC) remains one of the most devastating cancer types despite the improvement of modern medicine. In our present study, we found that dickkopf-related protein 2 (DKK2) shares a higher expression in PDAC compared with adjacent pancreas tissue in tissue microarray. In addition, an elevated expression of DKK2 predicts poorer prognosis of patients and positively correlated with poor tumor differentiation. Multivariate Cox regression analysis was also performed and confirmed that the expression of DKK2 is an independent prognostic factor in PDAC. A high expression of DKK2 correlates with cell migration and epithelial mesenchymal transition based on gene set enrichment analysis (GSEA) while knockdown of DKK2 in PDAC cells resulted in impaired cellular migration. Furthermore, GSEA predicts negative correlation between tumor immunity invasion and DKK2 expression. We then confirmed these results and demonstrated that a higher expression of DKK2 imparts the recruitment of CD8+ T cells. Our work suggested that DKK2 imparts tumor immune evasion and is associated with poor prognosis in pancreatic ductal adenocarcinoma. |
| format | Article |
| id | doaj-art-cd6564b3d2b9460bbc0744e603fe6260 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-cd6564b3d2b9460bbc0744e603fe62602025-02-03T01:13:02ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/86562828656282DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal AdenocarcinomaJianyu Yang0Yongsheng Jiang1Ruizhe He2Wei Liu3Minwei Yang4Lingye Tao5Xueliang Fu6Yang Shen7Jiao Li8Dejun Liu9Yanmiao Huo10Junfeng Zhang11Rong Hua12Zhigang Zhang13Yongwei Sun14Department of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Hepatobiliary Pancreas Surgery, Shanghai East Hospital, Tong Ji University School of Medicine, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaState Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biliary-Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaPancreatic ductal adenocarcinoma (PDAC) remains one of the most devastating cancer types despite the improvement of modern medicine. In our present study, we found that dickkopf-related protein 2 (DKK2) shares a higher expression in PDAC compared with adjacent pancreas tissue in tissue microarray. In addition, an elevated expression of DKK2 predicts poorer prognosis of patients and positively correlated with poor tumor differentiation. Multivariate Cox regression analysis was also performed and confirmed that the expression of DKK2 is an independent prognostic factor in PDAC. A high expression of DKK2 correlates with cell migration and epithelial mesenchymal transition based on gene set enrichment analysis (GSEA) while knockdown of DKK2 in PDAC cells resulted in impaired cellular migration. Furthermore, GSEA predicts negative correlation between tumor immunity invasion and DKK2 expression. We then confirmed these results and demonstrated that a higher expression of DKK2 imparts the recruitment of CD8+ T cells. Our work suggested that DKK2 imparts tumor immune evasion and is associated with poor prognosis in pancreatic ductal adenocarcinoma.http://dx.doi.org/10.1155/2019/8656282 |
| spellingShingle | Jianyu Yang Yongsheng Jiang Ruizhe He Wei Liu Minwei Yang Lingye Tao Xueliang Fu Yang Shen Jiao Li Dejun Liu Yanmiao Huo Junfeng Zhang Rong Hua Zhigang Zhang Yongwei Sun DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma Journal of Immunology Research |
| title | DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma |
| title_full | DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma |
| title_fullStr | DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma |
| title_full_unstemmed | DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma |
| title_short | DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma |
| title_sort | dkk2 impairs tumor immunity infiltration and correlates with poor prognosis in pancreatic ductal adenocarcinoma |
| url | http://dx.doi.org/10.1155/2019/8656282 |
| work_keys_str_mv | AT jianyuyang dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT yongshengjiang dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT ruizhehe dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT weiliu dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT minweiyang dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT lingyetao dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT xueliangfu dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT yangshen dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT jiaoli dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT dejunliu dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT yanmiaohuo dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT junfengzhang dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT ronghua dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT zhigangzhang dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma AT yongweisun dkk2impairstumorimmunityinfiltrationandcorrelateswithpoorprognosisinpancreaticductaladenocarcinoma |