Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics
This study compared the effects of seed treatment with low-pressure cold plasma (CP) and atmospheric dielectric barrier discharge (DBD) plasma on morpho-biochemical traits in <i>Stevia rabaudiana</i> Bertoni plants cultivated by two methods: in soil and aeroponics. We investigated the im...
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2025-01-01
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| author | Augustė Judickaitė Emilija Jankaitytė Evaldas Ramanciuškas Laima Degutytė-Fomins Zita Naučienė Gediminas Kudirka Takamasa Okumura Kazunori Koga Masaharu Shiratani Vida Mildažienė Rasa Žūkienė |
| author_facet | Augustė Judickaitė Emilija Jankaitytė Evaldas Ramanciuškas Laima Degutytė-Fomins Zita Naučienė Gediminas Kudirka Takamasa Okumura Kazunori Koga Masaharu Shiratani Vida Mildažienė Rasa Žūkienė |
| author_sort | Augustė Judickaitė |
| collection | DOAJ |
| description | This study compared the effects of seed treatment with low-pressure cold plasma (CP) and atmospheric dielectric barrier discharge (DBD) plasma on morpho-biochemical traits in <i>Stevia rabaudiana</i> Bertoni plants cultivated by two methods: in soil and aeroponics. We investigated the impact of the treatments on the germination, plant growth, and content of secondary metabolites, namely steviol glycosides (SGs), rebaudioside A (RebA), and stevioside (Stev), as well as phenolic compounds and flavonoids. Seeds were treated for 2, 5, and 7 min with CP or DBD and 5 min with vacuum six days before sowing. All growth parameters in aeroponics exceeded the parameters of seedlings in the corresponding groups cultivated in soil. Seed treatments stimulated SGs biosynthesis in seedlings grown in soil, except for CP7. Although there were no stimulating effects of seed treatments on SGs in aeroponics, overall SG concentrations were considerably higher compared to plants cultivated in soil: the RebA+Stev concentration was 1.8–2-fold higher in the control, V5-, and CP-treated groups, and 1.3-fold higher in the DBD5 and DBD7 groups. Thus, aeroponic cultivation has the potential to improve the growth and synthesis of SGs in stevia, while a combination of aeroponics with seed treatments only increases the content of antioxidants and antioxidant activity. |
| format | Article |
| id | doaj-art-cd5ff418908b43789b436b240e6422c0 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-cd5ff418908b43789b436b240e6422c02025-01-24T13:47:01ZengMDPI AGPlants2223-77472025-01-0114227110.3390/plants14020271Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus AeroponicsAugustė Judickaitė0Emilija Jankaitytė1Evaldas Ramanciuškas2Laima Degutytė-Fomins3Zita Naučienė4Gediminas Kudirka5Takamasa Okumura6Kazunori Koga7Masaharu Shiratani8Vida Mildažienė9Rasa Žūkienė10Faculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaUAB “Baltic Freya”, Tulpiu Str. 19, LT-53250 Garliava, LithuaniaDepartment of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Electronics, Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, JapanFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto Str. 10, LT-53361 Akademija, LithuaniaThis study compared the effects of seed treatment with low-pressure cold plasma (CP) and atmospheric dielectric barrier discharge (DBD) plasma on morpho-biochemical traits in <i>Stevia rabaudiana</i> Bertoni plants cultivated by two methods: in soil and aeroponics. We investigated the impact of the treatments on the germination, plant growth, and content of secondary metabolites, namely steviol glycosides (SGs), rebaudioside A (RebA), and stevioside (Stev), as well as phenolic compounds and flavonoids. Seeds were treated for 2, 5, and 7 min with CP or DBD and 5 min with vacuum six days before sowing. All growth parameters in aeroponics exceeded the parameters of seedlings in the corresponding groups cultivated in soil. Seed treatments stimulated SGs biosynthesis in seedlings grown in soil, except for CP7. Although there were no stimulating effects of seed treatments on SGs in aeroponics, overall SG concentrations were considerably higher compared to plants cultivated in soil: the RebA+Stev concentration was 1.8–2-fold higher in the control, V5-, and CP-treated groups, and 1.3-fold higher in the DBD5 and DBD7 groups. Thus, aeroponic cultivation has the potential to improve the growth and synthesis of SGs in stevia, while a combination of aeroponics with seed treatments only increases the content of antioxidants and antioxidant activity.https://www.mdpi.com/2223-7747/14/2/271aeroponicsantioxidant activitycold plasmaseedssecondary metabolites<i>Stevia rebaudiana</i> Bertoni |
| spellingShingle | Augustė Judickaitė Emilija Jankaitytė Evaldas Ramanciuškas Laima Degutytė-Fomins Zita Naučienė Gediminas Kudirka Takamasa Okumura Kazunori Koga Masaharu Shiratani Vida Mildažienė Rasa Žūkienė Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics Plants aeroponics antioxidant activity cold plasma seeds secondary metabolites <i>Stevia rebaudiana</i> Bertoni |
| title | Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics |
| title_full | Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics |
| title_fullStr | Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics |
| title_full_unstemmed | Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics |
| title_short | Effects of Seed Processing with Cold Plasma on Growth and Biochemical Traits of <i>Stevia rebaudiana</i> Bertoni Under Different Cultivation Conditions: In Soil Versus Aeroponics |
| title_sort | effects of seed processing with cold plasma on growth and biochemical traits of i stevia rebaudiana i bertoni under different cultivation conditions in soil versus aeroponics |
| topic | aeroponics antioxidant activity cold plasma seeds secondary metabolites <i>Stevia rebaudiana</i> Bertoni |
| url | https://www.mdpi.com/2223-7747/14/2/271 |
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