In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes
The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/864718 |
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| author | José Gutiérrez-Salinas Liliana García-Ortíz José A. Morales González Sergio Hernández-Rodríguez Sotero Ramírez-García Norma R. Núñez-Ramos Eduardo Madrigal-Santillán |
| author_facet | José Gutiérrez-Salinas Liliana García-Ortíz José A. Morales González Sergio Hernández-Rodríguez Sotero Ramírez-García Norma R. Núñez-Ramos Eduardo Madrigal-Santillán |
| author_sort | José Gutiérrez-Salinas |
| collection | DOAJ |
| description | The aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress.
For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 μg/mL) or NaF (100 μg/mL) + vitamin E (1, 2.5, 5 and 10 μg/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E. |
| format | Article |
| id | doaj-art-cd4ff57a647f45f39c91dc01834d3800 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cd4ff57a647f45f39c91dc01834d38002025-02-03T01:11:03ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/864718864718In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human ErythrocytesJosé Gutiérrez-Salinas0Liliana García-Ortíz1José A. Morales González2Sergio Hernández-Rodríguez3Sotero Ramírez-García4Norma R. Núñez-Ramos5Eduardo Madrigal-Santillán6Laboratory of Biochemistry and Experimental Medicine, Biomedical Research Division, National Medical Center “20 de Noviembre,” ISSSTE, Sn. Lorenzo No. 502, 2° piso, Col. Del Valle, 03100 México, DF, MexicoDivisión of Genomic Medicine, National Medical Center “20 de Noviembre,” ISSSTE, Sn. Lorenzo No. 502, 2° piso, Col. Del Valle, 03100 México, DF, MexicoLaboratorio Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, MexicoLaboratory of Biochemistry and Experimental Medicine, Biomedical Research Division, National Medical Center “20 de Noviembre,” ISSSTE, Sn. Lorenzo No. 502, 2° piso, Col. Del Valle, 03100 México, DF, MexicoUromédica OSF, Calle Uxmal 422, Colonia Vértiz Narvarte, 03020 México, DF, MexicoUromédica OSF, Calle Uxmal 422, Colonia Vértiz Narvarte, 03020 México, DF, MexicoLaboratorio Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, MexicoThe aim of this paper was to describe the in vitro effect of sodium fluoride (NaF) on the specific activity of the major erythrocyte antioxidant enzymes, as well as on the membrane malondialdehyde concentration, as indicators of oxidative stress. For this purpose, human erythrocytes were incubated with NaF (0, 7, 28, 56, and 100 μg/mL) or NaF (100 μg/mL) + vitamin E (1, 2.5, 5 and 10 μg/mL). The malondialdehyde (MDA) concentration on the surface of the erythrocytes was determined, as were the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GlPx). Our results demonstrated that erythrocytes incubated with increasing NaF concentrations had an increased MDA concentration, along with decreased activity of antioxidant enzymes. The presence of vitamin E partially reversed the toxic effects of NaF on erythrocytes. These findings suggest that NaF induces oxidative stress in erythrocytes in vitro, and this stress is partially reversed by the presence of vitamin E.http://dx.doi.org/10.1155/2013/864718 |
| spellingShingle | José Gutiérrez-Salinas Liliana García-Ortíz José A. Morales González Sergio Hernández-Rodríguez Sotero Ramírez-García Norma R. Núñez-Ramos Eduardo Madrigal-Santillán In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes The Scientific World Journal |
| title | In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes |
| title_full | In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes |
| title_fullStr | In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes |
| title_full_unstemmed | In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes |
| title_short | In Vitro Effect of Sodium Fluoride on Malondialdehyde Concentration and on Superoxide Dismutase, Catalase, and Glutathione Peroxidase in Human Erythrocytes |
| title_sort | in vitro effect of sodium fluoride on malondialdehyde concentration and on superoxide dismutase catalase and glutathione peroxidase in human erythrocytes |
| url | http://dx.doi.org/10.1155/2013/864718 |
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