Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation
This study explores the green extraction of phenolic antioxidants from <i>Aronia melanocarpa</i> fruit using choline-chloride-based deep eutectic solvents (DESs) as an eco-friendly alternative to conventional solvents. Sixteen DESs, prepared by combining choline chloride with various hyd...
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2024-12-01
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| Series: | Antioxidants |
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| author | Maja Molnar Martina Jakovljević Kovač Lidija Jakobek Lovro Mihajlović Valentina Pavić |
| author_facet | Maja Molnar Martina Jakovljević Kovač Lidija Jakobek Lovro Mihajlović Valentina Pavić |
| author_sort | Maja Molnar |
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| description | This study explores the green extraction of phenolic antioxidants from <i>Aronia melanocarpa</i> fruit using choline-chloride-based deep eutectic solvents (DESs) as an eco-friendly alternative to conventional solvents. Sixteen DESs, prepared by combining choline chloride with various hydrogen bond donors, were characterized for their physical properties, including viscosity, polarity, and pH, and applied to extract phenolics from <i>Aronia melanocarpa</i>. High-performance liquid chromatography (HPLC) quantified key phenolic compounds, including neochlorogenic and chlorogenic acid, quercetin derivatives, and cyanidin derivatives, as well as total phenolic acids, flavanols, and anthocyanins. The results revealed that DES composition and physical properties significantly influenced extraction efficiency and antioxidant activity. Additionally, the intrinsic antioxidant activity of DESs contributed substantially to the overall activity of the extracts, particularly in DESs containing organic acids or thiourea. Choline chloride/tartaric acid DES demonstrated the highest total phenolic content, attributed to its high viscosity and strongly acidic pH, while choline chloride/thiourea DES, with low viscosity and slightly acidic pH, exhibited the greatest antioxidant activity. This study highlights how tailoring DES formulations can optimize the extraction of target compounds while accounting for the solvent’s intrinsic properties. The findings support the potential application of DESs as environmentally friendly solvents in the food, pharmaceutical, and cosmetic industries. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-cd2bbb503a2f4c59837396551afc71392025-01-24T13:19:13ZengMDPI AGAntioxidants2076-39212024-12-011413110.3390/antiox14010031Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity InvestigationMaja Molnar0Martina Jakovljević Kovač1Lidija Jakobek2Lovro Mihajlović3Valentina Pavić4Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhača 18, 31000 Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaThis study explores the green extraction of phenolic antioxidants from <i>Aronia melanocarpa</i> fruit using choline-chloride-based deep eutectic solvents (DESs) as an eco-friendly alternative to conventional solvents. Sixteen DESs, prepared by combining choline chloride with various hydrogen bond donors, were characterized for their physical properties, including viscosity, polarity, and pH, and applied to extract phenolics from <i>Aronia melanocarpa</i>. High-performance liquid chromatography (HPLC) quantified key phenolic compounds, including neochlorogenic and chlorogenic acid, quercetin derivatives, and cyanidin derivatives, as well as total phenolic acids, flavanols, and anthocyanins. The results revealed that DES composition and physical properties significantly influenced extraction efficiency and antioxidant activity. Additionally, the intrinsic antioxidant activity of DESs contributed substantially to the overall activity of the extracts, particularly in DESs containing organic acids or thiourea. Choline chloride/tartaric acid DES demonstrated the highest total phenolic content, attributed to its high viscosity and strongly acidic pH, while choline chloride/thiourea DES, with low viscosity and slightly acidic pH, exhibited the greatest antioxidant activity. This study highlights how tailoring DES formulations can optimize the extraction of target compounds while accounting for the solvent’s intrinsic properties. The findings support the potential application of DESs as environmentally friendly solvents in the food, pharmaceutical, and cosmetic industries.https://www.mdpi.com/2076-3921/14/1/31chokeberryphenolic compoundsantioxidant activitydeep eutectic solventsextraction |
| spellingShingle | Maja Molnar Martina Jakovljević Kovač Lidija Jakobek Lovro Mihajlović Valentina Pavić Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation Antioxidants chokeberry phenolic compounds antioxidant activity deep eutectic solvents extraction |
| title | Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation |
| title_full | Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation |
| title_fullStr | Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation |
| title_full_unstemmed | Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation |
| title_short | Green Extraction of Phenolic Compounds from <i>Aronia melanocarpa</i> Using Deep Eutectic Solvents and Antioxidant Activity Investigation |
| title_sort | green extraction of phenolic compounds from i aronia melanocarpa i using deep eutectic solvents and antioxidant activity investigation |
| topic | chokeberry phenolic compounds antioxidant activity deep eutectic solvents extraction |
| url | https://www.mdpi.com/2076-3921/14/1/31 |
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