Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone

Cellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cell...

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Main Authors: B. Laamerad, P. Ansari
Format: Article
Language:English
Published: University of Tehran 2015-12-01
Series:Journal of Sciences, Islamic Republic of Iran
Subjects:
Online Access:https://jsciences.ut.ac.ir/article_55998_49a9692478bb5e2011312aea182ad479.pdf
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author B. Laamerad
P. Ansari
author_facet B. Laamerad
P. Ansari
author_sort B. Laamerad
collection DOAJ
description Cellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cellulases play a significant role in biotechnological processes. The cost of production of cellulase is very high. Approximately 50% of the cost of producing bioethanol is used to produce cellulose. Gibberellin regulates the growth of fungi and plants. Cellulase production was significantly improved by gibberellins hormone. The cellulase activity was 4.29 FPU/ml (when we used 50 μmol/ml of gibberellins in culture medium). This was 12- fold higher (0.34 FPU/ml in control solution) compare with untreated hormone and was comparable to that achieved with concentrated by n- butanol. In this experiment, the enzyme activity was increased 223- fold higher compare with control solution.
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institution OA Journals
issn 1016-1104
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language English
publishDate 2015-12-01
publisher University of Tehran
record_format Article
series Journal of Sciences, Islamic Republic of Iran
spelling doaj-art-cd1d2fe7ab5c46efa54360f8fbd1fd5f2025-08-20T02:25:54ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69142015-12-0126431531955998Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin HormoneB. Laamerad0P. Ansari1Department of Biology, Faculty of Sciences, Payame Noor University, Tehran, Islamic Republic of IranDepartment of Biology, Faculty of Sciences, Payame Noor University, Tehran, Islamic Republic of IranCellulolytic complex are enzymes capable of hydrolyzing cellulose. Due to rapid growth in population and industrialization, most countries are required to produce more fuel. Production of bioethanol from lignocellulosic biomass is very challenging due to environmental pollution by fossil fuels. Cellulases play a significant role in biotechnological processes. The cost of production of cellulase is very high. Approximately 50% of the cost of producing bioethanol is used to produce cellulose. Gibberellin regulates the growth of fungi and plants. Cellulase production was significantly improved by gibberellins hormone. The cellulase activity was 4.29 FPU/ml (when we used 50 μmol/ml of gibberellins in culture medium). This was 12- fold higher (0.34 FPU/ml in control solution) compare with untreated hormone and was comparable to that achieved with concentrated by n- butanol. In this experiment, the enzyme activity was increased 223- fold higher compare with control solution.https://jsciences.ut.ac.ir/article_55998_49a9692478bb5e2011312aea182ad479.pdftrichoderma reeseigibberellinscellulosebutanol
spellingShingle B. Laamerad
P. Ansari
Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
Journal of Sciences, Islamic Republic of Iran
trichoderma reesei
gibberellins
cellulose
butanol
title Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
title_full Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
title_fullStr Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
title_full_unstemmed Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
title_short Increased Production and Activity of Cellulase Enzyme of Trichoderma reesei by Using Gibberellin Hormone
title_sort increased production and activity of cellulase enzyme of trichoderma reesei by using gibberellin hormone
topic trichoderma reesei
gibberellins
cellulose
butanol
url https://jsciences.ut.ac.ir/article_55998_49a9692478bb5e2011312aea182ad479.pdf
work_keys_str_mv AT blaamerad increasedproductionandactivityofcellulaseenzymeoftrichodermareeseibyusinggibberellinhormone
AT pansari increasedproductionandactivityofcellulaseenzymeoftrichodermareeseibyusinggibberellinhormone